BRENDA - Enzyme Database
show all sequences of 1.8.99.5

Purification and characterization of an inducible dissimilatory type sulfite reductase from Clostridium pasteurianum

Harrison, G.; Curle, C.; Laishley, E.J.; Arch. Microbiol. 138, 72-78 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
KCN
1 mM, complete inhibition
Clostridium pasteurianum
additional information
not inhibitory: iodoacetate
Clostridium pasteurianum
NaN3
1 mM, 18% inhibition
Clostridium pasteurianum
Tris/HCl
Tris buffer has an inhibitory effect. Approximately 60% less activity than with phosphate buffer is obtained at pH 7.5
Clostridium pasteurianum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.35
-
sulfite
pH 7.5, 37°C
Clostridium pasteurianum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
13400
-
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
28400
-
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
83600
-
PAGE
Clostridium pasteurianum
Organism
Organism
UniProt
Commentary
Textmining
Clostridium pasteurianum
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+
-
394898
Clostridium pasteurianum
? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O
50% of the activity with sulfite
-
-
?
additional information
no substrates: S3O62-, S2O32-. Methyl viologen is as effective as ferredoxin in coupling the sulfite reductase with hydrogenase while benzyl viologen is only 16% as effective as ferredoxin. No activity is observed with NAD, NADP, FAD or FMN. Varying the substrate concentration [SO 2-] from 1 to 2.5 micromol affects the stoichiometry of the enzyme reaction by alteration of the ratio of H2 uptake to S2- formed from 2.5:1 to 3.1:1
394898
Clostridium pasteurianum
?
-
-
-
-
nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor
-
394898
Clostridium pasteurianum
? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O
25% of the activity with sulfite
-
-
?
SeO32- + a [DsrC protein]-dithiol + 2 reduced methyl viologen + 2 H+
-
394898
Clostridium pasteurianum
HSe- + a [DsrC protein]-disulfide + 2 oxidized methyl viologen + 3 H2O
40% of the activity with sulfite
-
-
?
sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+
-
394898
Clostridium pasteurianum
hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O
overall reaction
-
-
?
Subunits
Subunits
Commentary
Organism
heterotetramer
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
phosphate buffer
Clostridium pasteurianum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity is observed with NAD, NADP, FAD or FMN
Clostridium pasteurianum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity is observed with NAD, NADP, FAD or FMN
Clostridium pasteurianum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
KCN
1 mM, complete inhibition
Clostridium pasteurianum
additional information
not inhibitory: iodoacetate
Clostridium pasteurianum
NaN3
1 mM, 18% inhibition
Clostridium pasteurianum
Tris/HCl
Tris buffer has an inhibitory effect. Approximately 60% less activity than with phosphate buffer is obtained at pH 7.5
Clostridium pasteurianum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.35
-
sulfite
pH 7.5, 37°C
Clostridium pasteurianum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
13400
-
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
28400
-
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
83600
-
PAGE
Clostridium pasteurianum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
hydroxylamine + a [DsrC protein]-dithiol + 2 reduced acceptor + 2 H+
-
394898
Clostridium pasteurianum
? + a [DsrC protein]-disulfide + 2 acceptor + 3 H2O
50% of the activity with sulfite
-
-
?
additional information
no substrates: S3O62-, S2O32-. Methyl viologen is as effective as ferredoxin in coupling the sulfite reductase with hydrogenase while benzyl viologen is only 16% as effective as ferredoxin. No activity is observed with NAD, NADP, FAD or FMN. Varying the substrate concentration [SO 2-] from 1 to 2.5 micromol affects the stoichiometry of the enzyme reaction by alteration of the ratio of H2 uptake to S2- formed from 2.5:1 to 3.1:1
394898
Clostridium pasteurianum
?
-
-
-
-
nitrite + a [DsrC protein]-dithiol + 2 reduced acceptor
-
394898
Clostridium pasteurianum
? + a [DsrC protein]-disulfide + 2 acceptor + 2 H2O
25% of the activity with sulfite
-
-
?
SeO32- + a [DsrC protein]-dithiol + 2 reduced methyl viologen + 2 H+
-
394898
Clostridium pasteurianum
HSe- + a [DsrC protein]-disulfide + 2 oxidized methyl viologen + 3 H2O
40% of the activity with sulfite
-
-
?
sulfite + a [DsrC protein]-dithiol + 2 reduced ferredoxin + 2 H+
-
394898
Clostridium pasteurianum
hydrogen sulfide + a [DsrC protein]-disulfide + 2 oxidized ferredoxin + 3 H2O
overall reaction
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterotetramer
2 * 13400 plus 2 * 28400, SDS-PAGE
Clostridium pasteurianum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
phosphate buffer
Clostridium pasteurianum
Other publictions for EC 1.8.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741984
Duarte
Electron transfer between the ...
Desulfovibrio desulfuricans
Biochim. Biophys. Acta
1857
380-386
2016
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1
1
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733050
Ghosh
-
Intermolecular interaction stu ...
Allochromatium vinosum
AIDS Res. Hum. Retroviruses
340
19-27
2015
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742577
Leavitt
Sulfur isotope effects of dis ...
Archaeoglobus fulgidus, Desulfovibrio vulgaris, Desulfovibrio vulgaris DSM 644
Front. Microbiol.
6
1392
2015
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4
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2
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743347
Hermann
The octahaem MccA is a haem c ...
Wolinella succinogenes
Nature
520
706-709
2015
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1
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2
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2
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1
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1
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3
2
3
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2
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2
1
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2
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3
2
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3
3
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743053
Lee
-
Construction of aligned datab ...
Chlorobium phaeobacteroides, Chlorobium phaeobacteroides BS1, Chlorobium phaeobacteroides DSM 266, Desulfitobacterium dichloroeliminans, Desulfitobacterium dichloroeliminans LMG P-21439, Desulfosporosinus orientis, Desulfosporosinus orientis DSM 765, Desulfovibrio vulgaris, Desulfovibrio vulgaris DP4, Desulfovibrio vulgaris Hildenborough, Thermaerobacter marianensis, Thermaerobacter marianensis AB011495
J. Korean Soc. Appl. Biol. Chem.
57
419-427
2014
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12
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29
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12
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5
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12
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741792
Mori
Dominance of green sulfur bac ...
Candidatus Ruthia magnifica, Candidatus Thiobios zoothamnicoli, Chlorobium limicola, Chlorobium phaeovibrioides, Halochromatium salexigens, Magnetospirillum gryphiswaldense, Pelodictyon luteolum
Arch. Microbiol.
195
303-312
2013
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16
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741814
Venceslau
Redox states of Desulfovibrio ...
Desulfovibrio vulgaris
Biochem. Biophys. Res. Commun.
441
732-736
2013
-
-
1
-
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1
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2
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1
1
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725901
Holkenbrink
Sulfur globule oxidation in gr ...
Chlorobaculum tepidum, Chlorobaculum tepidum DSM 12025
Microbiology
157
1229-1239
2011
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5
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1
1
-
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710741
Ogata
Purification, crystallization ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Miyazaki F
Acta Crystallogr. Sect. F
66
1470-1472
2010
-
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1
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11
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710943
Moreau
Diversity of dissimilatory sul ...
Soil bacterium
Appl. Environ. Microbiol.
76
4819-4828
2010
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1
1
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688412
Schiffer
Structure of the dissimilatory ...
Archaeoglobus fulgidus
J. Mol. Biol.
379
1063-1074
2008
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1
1
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4
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688700
Oliveira
Purification, crystallization ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Hildenborough
J. Struct. Biol.
164
236-239
2008
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1
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698846
Oliveira
The crystal structure of Desul ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Hildenborough
J. Biol. Chem.
283
34141-34149
2008
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1
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673621
Mander
X-ray structure of the gamma-s ...
Archaeoglobus fulgidus
FEBS Lett.
579
4600-4604
2005
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733771
Cort
Solution structure of Pyrobacu ...
Pyrobaculum aerophilum, Pyrobaculum aerophilum DSM 7523
Eur. J. Biochem.
268
5842-5850
2001
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722213
Larsen
Dissimilatory sulfite reductas ...
Archaeoglobus profundus, Archaeoglobus profundus DSM 5631, Desulfofundulus thermocisternus
Extremophiles
3
63-70
1999
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10
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734636
Pott
-
Sirohaem sulfite reductase and ...
Allochromatium vinosum, Allochromatium vinosum DSM 180
Microbiology
144
1881-1894
1998
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2
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2
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1
1
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734637
Molitor
A dissimilatory sirohaem-sulfi ...
Pyrobaculum islandicum
Microbiology
144
529-541
1998
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1
4
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1
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4
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1
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733765
Marritt
Dissimilatory sulfite reductas ...
Desulfovibrio vulgaris
Eur. J. Biochem.
238
724-727
1996
-
-
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394066
Steuber
Molecular properties of the di ...
Desulfovibrio desulfuricans, Desulfovibrio desulfuricans Essex
Eur. J. Biochem.
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873-879
1995
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4
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4
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6
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1
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1
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1
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1
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4
1
4
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1
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1
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394063
Lui
Conformational gating of the d ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Hildenborough
Biochemistry
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11209-11216
1994
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18
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2
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2
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394068
Wolfe
Desulfoviridin, a multimeric-d ...
Desulfovibrio vulgaris, Desulfovibrio vulgaris Hildenborough
Eur. J. Biochem.
223
79-89
1994
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3
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22
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1
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6
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3
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2
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2
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3
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1
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6
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3
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3
3
394040
Seki
Characterization of a dissimil ...
Desulfocurvibacter africanus, Desulfocurvibacter africanus Benghazi
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1535-1543
1985
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2
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1
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1
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394898
Harrison
Purification and characterizat ...
Clostridium pasteurianum
Arch. Microbiol.
138
72-78
1984
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4
1
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3
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1
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5
1
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1
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1
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1
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4
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1
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3
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5
1
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1
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394043
Hatchikian
Characterization of a new type ...
Thermodesulfobacterium commune
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1211-1220
1983
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2
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