BRENDA - Enzyme Database
show all sequences of 1.8.98.1

A ferredoxin- and F420H2-dependent, electron-bifurcating, heterodisulfide reductase with homologs in the domains Bacteria and Archaea

Yan, Z.; Wang, M.; Ferry, J.G.; mBio 8, e02285-16 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
-
coenzyme B
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.2
-
coenzyme B
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.2
-
coenzyme M
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.4
-
coenzyme M
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
subunit HdrB2 contains zinc
Methanosarcina acetivorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanosarcina acetivorans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420
-
743210
Methanosarcina acetivorans
coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 18000, subunit HdrC2, SDS-PAGE; x * 33000, subunit HdrB2, SDS-PAGE; x * 87000, subunit HdrA2, SDS-PAGE
Methanosarcina acetivorans
Cofactor
Cofactor
Commentary
Organism
Structure
coenzyme F420
-
Methanosarcina acetivorans
FAD
-
Methanosarcina acetivorans
[2Fe-2S]-center
-
Methanosarcina acetivorans
[4Fe-4S]-center
-
Methanosarcina acetivorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Methanosarcina acetivorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
coenzyme F420
-
Methanosarcina acetivorans
FAD
-
Methanosarcina acetivorans
[2Fe-2S]-center
-
Methanosarcina acetivorans
[4Fe-4S]-center
-
Methanosarcina acetivorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
-
coenzyme B
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.2
-
coenzyme B
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.2
-
coenzyme M
subunit HdrB2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
1.4
-
coenzyme M
subunit HdrB2C2, at 21°C, pH not specified in the publication
Methanosarcina acetivorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
subunit HdrB2 contains zinc
Methanosarcina acetivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
coenzyme B + coenzyme M + reduced ferredoxin + H+ + coenzyme F420
-
743210
Methanosarcina acetivorans
coenzyme B-coenzyme M disulfide + ferredoxin + H2 + reduced coenzyme F420
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 18000, subunit HdrC2, SDS-PAGE; x * 33000, subunit HdrB2, SDS-PAGE; x * 87000, subunit HdrA2, SDS-PAGE
Methanosarcina acetivorans
Other publictions for EC 1.8.98.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743210
Yan
A ferredoxin- and F420H2-depe ...
Methanosarcina acetivorans
mBio
8
e02285-16
2017
-
-
1
-
-
-
-
4
-
1
-
-
-
3
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
-
-
-
-
1
4
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742500
Kroeninger
Evidence for the involvement ...
Methanomassiliicoccus luminyensis, Methanomassiliicoccus luminyensis DSM 25720
FEBS J.
283
472-483
2016
-
-
-
-
-
-
-
1
1
1
1
2
-
5
-
-
1
-
-
-
-
-
6
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
1
2
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
742541
Boughanemi
Microbial oxidative sulfur me ...
Aquifex aeolicus
FEMS Microbiol. Lett.
363
fnw156
2016
-
-
-
-
-
-
-
-
1
-
2
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742382
Ramos
The FlxABCD-HdrABC proteins c ...
Desulfovibrio vulgaris
Environ. Microbiol.
17
2288-2305
2015
-
-
1
-
-
-
-
-
-
1
-
4
-
4
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725292
Costa
VhuD facilitates electron flow ...
Methanococcus maripaludis
J. Bacteriol.
195
5160-5165
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725558
Fielding
Advanced electron paramagnetic ...
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
J. Biol. Inorg. Chem.
18
905-915
2013
1
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741554
Ehrenfeld
-
Heterodisulfide reductase fro ...
Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans DSM 16786, Acidithiobacillus thiooxidans, Acidithiobacillus thiooxidans DSM 17318
Adv. Mater. Res.
825
194-197
2013
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
713055
Buan
Methanogenesis by Methanosarci ...
Methanosarcina acetivorans
Mol. Microbiol.
75
843-853
2010
-
-
1
-
1
-
-
-
1
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
713413
Costa
Protein complexing in a methan ...
Methanococcus maripaludis, Methanococcus maripaludis MM1265
Proc. Natl. Acad. Sci. USA
107
11050-11055
2010
-
-
1
-
1
-
-
-
-
-
-
4
-
5
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
685106
Hamann
A cysteine-rich CCG domain con ...
Methanothermobacter marburgensis
Biochemistry
46
12875-12885
2007
-
-
1
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667229
Zhang
Global transcriptomic analysis ...
Desulfovibrio vulgaris
Antonie van Leeuwenhoek
89
221-237
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667716
Pires
Characterization of the Desulf ...
Desulfovibrio desulfuricans
Biochemistry
45
249-262
2006
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655593
Shokes
Direct interaction of coenzyme ...
Methanothermobacter marburgensis
FEBS Lett.
579
1741-1744
2005
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667866
Hedderich
Heterodisulfide reductase from ...
Methanosarcina barkeri, Methanosarcina thermophila, Methanothermobacter marburgensis
Biol. Chem.
386
961-970
2005
-
-
-
-
-
-
3
-
-
3
-
-
-
4
-
-
3
-
-
-
-
-
6
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
-
-
3
-
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655471
de Poorter
Bioenergetics of the formyl-me ...
Methanothermobacter thermautotrophicus
Eur. J. Biochem.
270
66-75
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655568
Duin
Coenzyme M binds to a [4Fe-4S] ...
Methanosarcina barkeri, Methanothermobacter marburgensis
FEBS Lett.
538
81-84
2003
-
-
-
-
-
-
-
-
-
2
-
-
-
6
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655551
Duin
Heterodisulfide reductase from ...
Methanothermobacter marburgensis
FEBS Lett.
512
263-268
2002
-
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680622
Iwasaki
Novel [2Fe-2S]-type redox cent ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
J. Biol. Chem.
277
39642-39648
2002
-
-
1
1
-
-
1
-
-
2
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639394
Murakami
Characterization of the intram ...
Methanosarcina thermophila
J. Biol. Chem.
276
2432-2439
2001
-
-
-
-
-
-
1
2
-
1
-
2
-
3
-
-
-
-
-
-
1
-
4
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
1
-
2
-
-
-
-
-
-
1
-
4
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
655416
Madadi-Kahkesh
A paramagnetic species with un ...
Methanosarcina barkeri, Methanothermobacter marburgensis
Eur. J. Biochem.
268
2566-2577
2001
-
-
1
-
-
-
4
-
-
-
-
-
-
5
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654386
Brodersen
Membrane-bound F420H2-dependen ...
Methanococcus voltae
Arch. Microbiol.
171
115-121
1999
-
-
-
-
-
-
-
-
1
-
-
1
-
3
1
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655825
Ide
Energy conservation by the H2: ...
Methanosarcina mazei
J. Bacteriol.
181
4076-4080
1999
-
-
-
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440299
Abken
Isolation and characterization ...
Methanosarcina mazei
J. Bacteriol.
180
2027-2032
1998
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639393
Simianu
Purification and properties of ...
Methanosarcina thermophila
Biochemistry
37
10027-10039
1998
-
-
-
-
-
-
-
-
1
1
4
1
-
4
-
1
1
-
-
1
1
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
4
1
-
-
1
1
-
1
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655402
Kunkel
Heterodisulfide reductase from ...
Methanosarcina barkeri
Eur. J. Biochem.
244
226-234
1997
-
-
1
-
-
-
-
-
1
-
2
-
-
5
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
2
-
1
-
-
-
1
2
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
655398
Setzke
H2:heterodisulfide oxidoreduct ...
Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus Marburg / DSM 2133
Eur. J. Biochem.
220
139-148
1994
1
-
-
-
-
-
-
2
1
2
2
2
-
33
-
-
1
-
-
-
-
-
4
1
-
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
2
1
2
2
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
655400
Heiden
Purification of a two-subunit ...
Methanosarcina barkeri
Eur. J. Biochem.
221
855-861
1994
-
-
-
-
-
1
-
3
1
1
2
1
-
4
-
-
1
-
-
1
1
-
3
1
1
-
-
-
-
1
-
2
-
-
-
-
-
-
2
-
-
1
-
-
-
3
1
1
2
1
-
-
-
1
-
1
1
-
3
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
655823
Peer
Characterization of a CO:heter ...
Methanosarcina thermophila
J. Bacteriol.
176
6974-6979
1994
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655396
Heiden
Purification of a cytochrome b ...
Methanosarcina barkeri
Eur. J. Biochem.
213
529-535
1993
-
-
-
-
-
-
-
-
3
2
1
1
-
4
-
-
1
-
-
-
2
-
3
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
2
1
1
-
-
-
1
-
-
2
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654385
Deppenmeier
-
H2:heterodisulfide oxidoreduct ...
Methanosarcina mazei
Arch. Microbiol.
155
272-277
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639391
Hedderich
Purification and properties of ...
Methanosarcina barkeri, Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus Marburg / DSM 2133
Eur. J. Biochem.
193
255-261
1990
-
-
-
-
-
-
1
6
-
-
5
2
-
35
1
-
1
-
-
-
4
1
14
2
1
-
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
6
-
-
5
2
-
1
-
1
-
-
4
1
14
2
1
-
1
1
-
-
-
-
-
-
-
-
-
-
655532
Deppenmeier
-
Membrane-bound F420H2-dependen ...
Methanolobus tindarius, Methanosarcina mazei
FEBS Lett.
261
199-203
1990
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657172
Deppenmeier
Reduced coenzyme F420: heterod ...
Methanosarcina mazei
Proc. Natl. Acad. Sci. USA
87
9449-9453
1990
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655531
Hedderich
-
Methanobacterium thermoautotro ...
Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus Marburg / DSM 2133
FEBS Lett.
234
223-227
1988
-
-
-
-
-
-
-
1
1
-
-
-
-
31
-
-
1
-
-
-
1
1
2
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
1
1
2
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-