Literature summary for 1.8.5.4 extracted from
Chen, X.; Meng, Z.; Luo, Y.; Rong, X.; Li, S.
Structure and activity of sulfide quinone oxidoreductase and its mutants from Thiobacillus denitrificans ATCC 25259 (2017), Mod. Food Sci. Technol., 33, 46-55 .
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene sqr, recombinant expression of His6-tagged wild-type enzyme and mutants in Escherichia coli |
Thiobacillus denitrificans |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
construction of enzyme mutants with reduced activity compared to wild-type |
Thiobacillus denitrificans |
Organism
Organism |
UniProt |
Comment |
Textmining |
Thiobacillus denitrificans |
Q8KQ39 |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant His6-tagged wild-type enzyme and mutants from Escherichia coli by nickel affinity chromatography |
Thiobacillus denitrificans |
Synonyms
Synonyms |
Comment |
Organism |
SQR |
- |
Thiobacillus denitrificans |
sulfide:quinone oxidoreductase |
- |
Thiobacillus denitrificans |
General Information
General Information |
Comment |
Organism |
additional information |
structure homology modelling, molecular mechanics and molecular dynamics calculations, overview. Analysis of interactions between the protein and ligands, calculation of solvent accessible surface (SAS) and energy value. The structural stability of the alpha-helix in the C-terminus of enzyme SQR has an important influence on the structural stability of the whole protein |
Thiobacillus denitrificans |