BRENDA - Enzyme Database show
show all sequences of 1.8.4.9

Sulfate reduction is increased in transgenic Arabidopsis thaliana expressing 5'-adenylylsulfate reductase from Pseudomonas aeruginosa

Tsakraklides, G.; Martin, M.; Chalam, R.; Tarczynski, M.C.; Schmidt, A.; Leustek, T.; Plant J. 32, 879-889 (2002)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in wild-type plants
Arabidopsis thaliana
Cloned(Commentary)
Commentary
Organism
recombinant expression of the enzyme, fused to the transit peptide rbcS, in Arabidopsis thaliana under transcriptional control of the CaMV 35S promotor
Pseudomonas aeruginosa
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview
Arabidopsis thaliana
additional information
construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview
Pseudomonas aeruginosa
General Stability
General Stability
Organism
enzyme is highly stable in vitro
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
x * 29000, recombinant enzyme, SDS-PAGE
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5'-adenylyl sulfate + glutathione
Arabidopsis thaliana
enzyme is involved in reductive sulfate assimilation, pathway overview
AMP + sulfite + glutathione disulfide
-
-
?
5'-adenylyl sulfate + glutathione
Pseudomonas aeruginosa
enzyme is involved in reductive sulfate assimilation, pathway overview
AMP + sulfite + glutathione disulfide
-
-
?
5'-adenylyl sulfate + thioredoxin
Pseudomonas aeruginosa
recombinant enzyme in transgenic Arabidopsis thaliana plants
AMP + sulfite + thioredoxin disulfide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Pseudomonas aeruginosa
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00077
-
wild-type plants
Arabidopsis thaliana
0.0023
0.006
transgenic plants with the recombinant Pseudomonas aeruginosa enzyme utilizing thioredoxin
Arabidopsis thaliana
0.0023
0.006
recombinant enzyme in transgenic Arabidopsis thaliana plants, cofactor thioredoxin
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-adenylyl sulfate + glutathione
-
660168
Arabidopsis thaliana
AMP + sulfite + glutathione disulfide
-
-
-
?
5'-adenylyl sulfate + glutathione
-
660168
Pseudomonas aeruginosa
AMP + sulfite + glutathione disulfide
-
-
-
?
5'-adenylyl sulfate + glutathione
enzyme is involved in reductive sulfate assimilation, pathway overview
660168
Arabidopsis thaliana
AMP + sulfite + glutathione disulfide
-
-
-
?
5'-adenylyl sulfate + glutathione
enzyme is involved in reductive sulfate assimilation, pathway overview
660168
Pseudomonas aeruginosa
AMP + sulfite + glutathione disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
recombinant enzyme in transgenic Arabidopsis thaliana plants
660168
Pseudomonas aeruginosa
AMP + sulfite + thioredoxin disulfide
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 29000, recombinant enzyme, SDS-PAGE
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
glutathione
-
Pseudomonas aeruginosa
glutathione
-
Arabidopsis thaliana
additional information
no activity with thioredoxin
Arabidopsis thaliana
thioredoxin
recombinant enzyme utilizes the plant thioredoxins m and f in transgenic plants
Pseudomonas aeruginosa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in wild-type plants
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of the enzyme, fused to the transit peptide rbcS, in Arabidopsis thaliana under transcriptional control of the CaMV 35S promotor
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
glutathione
-
Pseudomonas aeruginosa
glutathione
-
Arabidopsis thaliana
additional information
no activity with thioredoxin
Arabidopsis thaliana
thioredoxin
recombinant enzyme utilizes the plant thioredoxins m and f in transgenic plants
Pseudomonas aeruginosa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview
Arabidopsis thaliana
additional information
construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview
Pseudomonas aeruginosa
General Stability (protein specific)
General Stability
Organism
enzyme is highly stable in vitro
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
x * 29000, recombinant enzyme, SDS-PAGE
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5'-adenylyl sulfate + glutathione
Arabidopsis thaliana
enzyme is involved in reductive sulfate assimilation, pathway overview
AMP + sulfite + glutathione disulfide
-
-
?
5'-adenylyl sulfate + glutathione
Pseudomonas aeruginosa
enzyme is involved in reductive sulfate assimilation, pathway overview
AMP + sulfite + glutathione disulfide
-
-
?
5'-adenylyl sulfate + thioredoxin
Pseudomonas aeruginosa
recombinant enzyme in transgenic Arabidopsis thaliana plants
AMP + sulfite + thioredoxin disulfide
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00077
-
wild-type plants
Arabidopsis thaliana
0.0023
0.006
transgenic plants with the recombinant Pseudomonas aeruginosa enzyme utilizing thioredoxin
Arabidopsis thaliana
0.0023
0.006
recombinant enzyme in transgenic Arabidopsis thaliana plants, cofactor thioredoxin
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-adenylyl sulfate + glutathione
-
660168
Arabidopsis thaliana
AMP + sulfite + glutathione disulfide
-
-
-
?
5'-adenylyl sulfate + glutathione
-
660168
Pseudomonas aeruginosa
AMP + sulfite + glutathione disulfide
-
-
-
?
5'-adenylyl sulfate + glutathione
enzyme is involved in reductive sulfate assimilation, pathway overview
660168
Arabidopsis thaliana
AMP + sulfite + glutathione disulfide
-
-
-
?
5'-adenylyl sulfate + glutathione
enzyme is involved in reductive sulfate assimilation, pathway overview
660168
Pseudomonas aeruginosa
AMP + sulfite + glutathione disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
recombinant enzyme in transgenic Arabidopsis thaliana plants
660168
Pseudomonas aeruginosa
AMP + sulfite + thioredoxin disulfide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 29000, recombinant enzyme, SDS-PAGE
Pseudomonas aeruginosa
Other publictions for EC 1.8.4.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743157
Chung
-
The Arabidopsis thaliana aden ...
Arabidopsis thaliana
J. Plant Biol.
58
128-136
2015
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1
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1
1
1
1
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-
689431
Phartiyal
The role of 5-adenylylsulfate ...
Glycine max
Phytochemistry
69
356-364
2008
-
-
1
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2
4
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1
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1
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1
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1
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2
1
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4
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1
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1
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2
1
4
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1
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1
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1
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2
1
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4
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694712
Koprivova
Complex signaling network in r ...
Arabidopsis thaliana
Plant Physiol.
146
1408-1420
2008
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1
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1
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2
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1
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2
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667746
Kim
The two-domain structure of 5- ...
Ulva intestinalis
Biochemistry
46
591-601
2007
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1
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2
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4
-
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1
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bacteria endosymbiont of Lucinoma aff. kazani
FEMS Microbiol. Ecol.
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64-70
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687585
Kopriva
The putative moss 3'-phosphoad ...
Physcomitrella patens
J. Biol. Chem.
282
22930-22938
2007
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1
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3
2
1
7
2
2
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8
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1
1
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4
8
1
1
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2
2
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2
1
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1
2
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3
1
2
1
7
2
2
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1
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4
8
1
1
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2
2
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694695
Wiedemann
The role of the novel adenosin ...
Physcomitrella patens
Plant Mol. Biol.
65
667-676
2007
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1
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1
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2
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1
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1
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2
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1
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1
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667703
Sun
Channeling in sulfate activati ...
Mycobacterium tuberculosis
Biochemistry
45
11304-11311
2006
-
-
1
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2
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1
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1
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2
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2
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667717
Schiffer
Reaction mechanism of the iron ...
Archaeoglobus fulgidus
Biochemistry
45
2960-2967
2006
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1
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2
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2
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1
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669891
Chartron
Substrate recognition, protein ...
Pseudomonas aeruginosa
J. Mol. Biol.
364
152-169
2006
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2
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1
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1
1
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670319
Senaratne
5-Adenosinephosphosulphate red ...
Mycobacterium tuberculosis
Mol. Microbiol.
59
1744-1753
2006
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660168
Tsakraklides
Sulfate reduction is increased ...
Arabidopsis thaliana, Pseudomonas aeruginosa
Plant J.
32
879-889
2002
1
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660113
Kopriva
-
Influence of chilling stress o ...
Zea mays
Plant Biol.
3
24-31
2001
1
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288643
Gao
Characterization of sulfate as ...
Arabidopsis thaliana, Brassica juncea, Conticribra weissflogii, Dunaliella salina, Emiliania huxleyi, Heterocapsa triquetra, Isochrysis galbana, Tetraselmis sp., Thalassiosira oceanica, Ulva intestinalis
Plant Physiol.
123
1087-1096
2000
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10
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12
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20
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1
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288644
Suter
Adenosine 5'-phosphosulfate su ...
Lemna minor
J. Biol. Chem.
275
930-936
2000
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1
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3
3
1
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3
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1
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288642
Prior
Structural and kinetic propert ...
Catharanthus roseus
Biochim. Biophys. Acta
1430
25-38
1999
1
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1
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1
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1
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660399
Bick
Glutaredoxin function for the ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
95
8404-8409
1998
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1
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3
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1
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1
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288640
Setya
Sulfate reduction in higher pl ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
93
13383-13388
1996
3
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1
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288641
Gutierrez-Marcos
Three members of a novel small ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
93
13377-13382
1996
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