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Literature summary for 1.8.4.2 extracted from
- Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii kinetics and specificity in deglutathionylation reactions (2010), FEBS Lett., 584, 2242-2248 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0009 | - |
glutathionylated glyceraldehyde-3-phosphate dehydrogenase | cosubstrate glutathione, S0.5 value, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 0.0023 | - |
glutathionylated glyceraldehyde-3-phosphate dehydrogenase | cosubstrate glutathione, S0.5 value, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 0.0082 | - |
glutathionylated isocitrate lyase | cosubstrate dithiothreitol, S0.5 value, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 0.012 | - |
glutathionylated isocitrate lyase | cosubstrate glutathione, S0.5 value, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 0.0164 | - |
glutathionylated isocitrate lyase | cosubstrate dithiothreitol, S0.5 value, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 0.0265 | - |
glutathionylated isocitrate lyase | cosubstrate glutathione, S0.5 value, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 0.2 | - |
2-mercaptoethanol glutathione disulfide | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 0.34 | - |
2-mercaptoethanol glutathione disulfide | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 2.65 | - |
glutathione | cosubstrate 2-mercaptoethanol glutathione disulfide, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii |  |
| 3.7 | 5 | glutathione | cosubstrate 2-mercaptoethanol glutathione disulfide, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Chlamydomonas reinhardtii | 5829 | - |
| mitochondrion | - |
Chlamydomonas reinhardtii | 5739 | - |
| plastid | - |
Chlamydomonas reinhardtii | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
PAGE | Chlamydomonas reinhardtii |
| 32000 | - |
PAGE | Chlamydomonas reinhardtii |
| 52000 | - |
PAGE | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | A8HN52 | isoform GRX6 | - |
| Chlamydomonas reinhardtii | A8I7Q4 | isoform GRX5 | - |
| Chlamydomonas reinhardtii | A8IYH1 | isoform GRX2 | - |
| Chlamydomonas reinhardtii | A8JHA9 | isoform GRX1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 glutathione + insulin disulfide | - |
Chlamydomonas reinhardtii | glutathione disulfide + insulin | - |
? | |
| 2-mercaptoethanol glutathione disulfide + glutathione | - |
Chlamydomonas reinhardtii | 2-mercaptoethanol disulfide + glutathione disulfide | - |
? | |
| dithiothreitol + insulin disulfide | - |
Chlamydomonas reinhardtii | dithiothreitol disulfide + insulin | - |
? | |
| glutathionylated glyceraldehyde-3-phosphate dehydrogenase + glutathione | substrate is glutathionylated A4-GAPDH. A4-GAPDH activity is reversibly inhibited by glutathionylation | Chlamydomonas reinhardtii | glutathione disulfide + glyceraldehyde-3-phosphate dehydrogenase | - |
? | |
| glutathionylated isocitrate lyase + dithiothreitol | - |
Chlamydomonas reinhardtii | glutathione dithiothreitol disulfide + isocitrate lyase | - |
? | |
| glutathionylated isocitrate lyase + glutathione | - |
Chlamydomonas reinhardtii | glutathione disulfide + isocitrate lyase | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 26000, SDS-PAGE | Chlamydomonas reinhardtii |
| dimer | 2 * 16000, SDS-PAGE | Chlamydomonas reinhardtii |
| monomer | 1 * 12000, SDS-PAGE | Chlamydomonas reinhardtii |
| More | formation of an intramolecular disulfide between Cys27 and Cys30 within the CPYC active site of GRX2 | Chlamydomonas reinhardtii |
| More | GRX5 can form covalent homodimers through intermolecular disulfide bonds | Chlamydomonas reinhardtii |
| More | GRX6 can form covalent homodimers through intermolecular disulfide bonds | Chlamydomonas reinhardtii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GRX1 | - |
Chlamydomonas reinhardtii |
| GRX2 | - |
Chlamydomonas reinhardtii |
| GRX5 | - |
Chlamydomonas reinhardtii |
| GRX6 | - |
Chlamydomonas reinhardtii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.1 | - |
2-mercaptoethanol glutathione disulfide | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 26.5 | - |
glutathione | cosubstrate 2-mercaptoethanol glutathione disulfide, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 30.4 | - |
2-mercaptoethanol glutathione disulfide | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 161 | - |
glutathione | cosubstrate 2-mercaptoethanol glutathione disulfide, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isoform GRX5 is not an efficient catalyst of protein deglutathionylation nor exhibits distinct substrate specificities | Chlamydomonas reinhardtii |
| physiological function | isoform GRX6 is not an efficient catalyst of protein deglutathionylation nor exhibits distinct substrate specificities | Chlamydomonas reinhardtii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.1 | - |
glutathione | cosubstrate 2-mercaptoethanol glutathione disulfide, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 35 | - |
2-mercaptoethanol glutathione disulfide | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 61 | - |
glutathione | cosubstrate 2-mercaptoethanol glutathione disulfide, pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
| 89 | - |
2-mercaptoethanol glutathione disulfide | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii |
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