Literature summary for 1.8.1.14 extracted from

Lencina, A.M.; Koepke, J.; Preu, J.; Muenke, C.; Gennis, R.B.; Michel, H.; Schurig-Briccio, L.A.
Characterization and X-ray structure of the NADH-dependent coenzyme A disulfide reductase from Thermus thermophilus (2019), Biochim. Biophys. Acta Bioenerg., 1860, 148080 .

Search for more literature for 1.8.1.14

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization by vapor diffusion. X-ray structure is determined at 2.9 A resolution	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0025	-	CoA-disulfide	pH 7.5, 42°C	Thermus thermophilus
0.072	-	NADH	pH 7.5, 42°C	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q72HK3	-	-
Thermus thermophilus ATCC BAA-163	Q72HK3	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+	-	Thermus thermophilus	2-nitro-5-thiobenzoate + NAD+	-	?
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+	-	Thermus thermophilus ATCC BAA-163	2-nitro-5-thiobenzoate + NAD+	-	?
CoA-disulfide + NADH + H+	the specific activity with CoA-disulfide as an electron acceptor is about 5fold higher than with menadione	Thermus thermophilus	2 CoA + NAD+	-	?
CoA-disulfide + NADH + H+	the specific activity with CoA-disulfide as an electron acceptor is about 5fold higher than with menadione	Thermus thermophilus ATCC BAA-163	2 CoA + NAD+	-	?
menadione + NADH + H+	-	Thermus thermophilus	? + NAD+	-	?
menadione + NADH + H+	-	Thermus thermophilus ATCC BAA-163	? + NAD+	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
CoADR	-	Thermus thermophilus
coenzyme A-disulfide reductase	-	Thermus thermophilus
NADH-dependent coenzyme A disulfide reductase	-	Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5	-	NADH	pH 7.5, 42°C	Thermus thermophilus
5	-	CoA-disulfide	pH 7.5, 42°C	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Thermus thermophilus
NADH	the enzyme exclusively oxidizes NADH	Thermus thermophilus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)