BRENDA - Enzyme Database show
show all sequences of 1.8.1.13

The novel disulfide reductase bis-gamma-glutamylcystine reductase and dihydrolipoamide dehydrogenase from Halobacterium halobium: Purification by immobilized-metal-ion affinity chromatography and properties of the enzymes

Sundquist, A.R.; Fahey, R.C.; J. Bacteriol. 170, 3459-3467 (1988)

Data extracted from this reference:

General Stability
General Stability
Organism
high-ionic-strength buffer above 2 M stabilizes during purification and storage
Halobacterium salinarum
high-ionic-strength buffer above 3.5 M increases thermal stability
Halobacterium salinarum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
2 * 61000, SDS-PAGE
Halobacterium salinarum
122000
-
gel filtration
Halobacterium salinarum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bis-gamma-glutamylcystine + NADPH + H+
Halobacterium salinarum
-
2 gamma-glutamylcysteine + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Halobacterium salinarum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Halobacterium salinarum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
25
-
-
Halobacterium salinarum
Storage Stability
Storage Stability
Organism
20C, stable to prolonged storage at room temperature in high-ionic-strength buffer above 3.5 M
Halobacterium salinarum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bis-gamma-glutamylcystine + NADPH + H+
-
392950
Halobacterium salinarum
2 gamma-glutamylcysteine + NADP+
-
-
-
?
additional information
no activity detected with GSSG
392950
Halobacterium salinarum
?
-
-
-
-
additional information
lipoic acid disulfide and dihydrolipoamide are no substrates
392950
Halobacterium salinarum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 61000, SDS-PAGE
Halobacterium salinarum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
retains full activity after incubation in high-and intermediate-ionic-strength buffers, inactivated after incubation in low-ionic strength buffers
Halobacterium salinarum
80
-
inactivation in buffers of each of the 3 ionic strengths, low-ionic, intermediate-ionic and high-ionic
Halobacterium salinarum
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Halobacterium salinarum
NADH
less effective than NADPH
Halobacterium salinarum
NADPH
-
Halobacterium salinarum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Halobacterium salinarum
NADH
less effective than NADPH
Halobacterium salinarum
NADPH
-
Halobacterium salinarum
General Stability (protein specific)
General Stability
Organism
high-ionic-strength buffer above 2 M stabilizes during purification and storage
Halobacterium salinarum
high-ionic-strength buffer above 3.5 M increases thermal stability
Halobacterium salinarum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
2 * 61000, SDS-PAGE
Halobacterium salinarum
122000
-
gel filtration
Halobacterium salinarum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bis-gamma-glutamylcystine + NADPH + H+
Halobacterium salinarum
-
2 gamma-glutamylcysteine + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Halobacterium salinarum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
25
-
-
Halobacterium salinarum
Storage Stability (protein specific)
Storage Stability
Organism
20C, stable to prolonged storage at room temperature in high-ionic-strength buffer above 3.5 M
Halobacterium salinarum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bis-gamma-glutamylcystine + NADPH + H+
-
392950
Halobacterium salinarum
2 gamma-glutamylcysteine + NADP+
-
-
-
?
additional information
no activity detected with GSSG
392950
Halobacterium salinarum
?
-
-
-
-
additional information
lipoic acid disulfide and dihydrolipoamide are no substrates
392950
Halobacterium salinarum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 61000, SDS-PAGE
Halobacterium salinarum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
retains full activity after incubation in high-and intermediate-ionic-strength buffers, inactivated after incubation in low-ionic strength buffers
Halobacterium salinarum
80
-
inactivation in buffers of each of the 3 ionic strengths, low-ionic, intermediate-ionic and high-ionic
Halobacterium salinarum
Other publictions for EC 1.8.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724378
Kim
The orphan protein bis-?-gluta ...
Halobacterium salinarum, Halobacterium salinarum NRC 1
Biochemistry
52
2905-2913
2013
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1
-
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-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
1
1
-
1
1
392951
Sundquist
The function of gamma-glutamyl ...
Halobacterium salinarum
J. Biol. Chem.
264
719-725
1989
-
-
-
-
-
1
4
2
1
-
2
1
-
3
-
-
1
-
-
-
1
-
4
1
-
-
-
1
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
2
1
-
2
1
-
-
-
1
-
-
1
-
4
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
392950
Sundquist
The novel disulfide reductase ...
Halobacterium salinarum
J. Bacteriol.
170
3459-3467
1988
-
-
-
-
-
2
-
-
-
-
2
1
-
2
-
-
1
-
-
-
1
1
3
1
-
-
2
-
-
-
-
3
-
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
1
3
1
-
-
2
-
-
-
-
-
-
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