Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leishmania amazonensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
native enzyme in promastigotes, SDS-PAGE, Western Blot | Leishmania amazonensis |
69200 | - |
recombinant fusion protein, SDS-PAGE | Leishmania amazonensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania amazonensis | Q0GU43 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein by Ni2+-charged chelating sepharose fast flow | Leishmania amazonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypanothione + 5,5'-dithiobis-(2-nitrobenzoic acid) | inhibitors are tested using the recombinant enzyme | Leishmania amazonensis | trypanothione disulfide + thionitrobenzoate | - |
? | |
trypanothione + NADP+ | parasite soluble fraction | Leishmania amazonensis | trypanothione disulfide + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trypanothione reductase | TR | Leishmania amazonensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
5,5-dithiobis-(2-nitrobenzoic acid) as substrate | Leishmania amazonensis |