Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23600 | - |
2 * 23600, SDS-PAGE | Geobacillus stearothermophilus |
46000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q9X4K2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
cosubstrate NADPH, pH 7.4, 30°C | Geobacillus stearothermophilus |
25.8 | - |
cosubstrate NADH, pH 7.4, 30°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-pyridylazo)-2-naphthol + NADH + H+ | - |
Geobacillus stearothermophilus | ? + NAD+ | - |
ir | |
acid red 88 + NADH + H+ | 10% of hte activity with methyl red at 30°C | Geobacillus stearothermophilus | ? + NAD+ | - |
ir | |
Congo red + NADH + H+ | - |
Geobacillus stearothermophilus | ? + NAD+ | - |
ir | |
ethyl red + NADH + H+ | best substrate, 182% of the activity with methyl red at 30°C | Geobacillus stearothermophilus | ? + NAD+ | - |
ir | |
methyl red + 2 NADH + 2 H+ | - |
Geobacillus stearothermophilus | o-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ | - |
ir | |
orange I + NADH + H+ | - |
Geobacillus stearothermophilus | ? + NAD+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23600, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
AzrG | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
one month, 67% residual activity | Geobacillus stearothermophilus |
60 | - |
10 min, no loss of activity | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Geobacillus stearothermophilus | |
additional information | no substrates: NADP+, NAD+ | Geobacillus stearothermophilus | |
NADH | NADH preferred over NADPH | Geobacillus stearothermophilus | |
NADPH | NADH is preferred over NADPH | Geobacillus stearothermophilus |