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Literature summary for 1.6.3.2 extracted from

  • Castillo-Villanueva, A.; Mendez, S.T.; Torres-Arroyo, A.; Reyes-Vivas, H.; Oria-Hernandez, J.
    Cloning, expression and characterization of recombinant, NADH oxidase from Giardia lamblia (2016), Protein J., 35, 24-33 .
    View publication on PubMed
Search for more literature for 1.6.3.2

Activating Compound

Activating Compound Comment Organism Structure
additional information not activated by dithiothreitol Giardia intestinalis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Giardia intestinalis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 92% inhibition at 0.1 mg/ml Giardia intestinalis
Hg2+ complete inhibition at 0.1 mg/ml Giardia intestinalis
p-mercuribenzoate 99% inhibition at 0.1 mg/ml Giardia intestinalis
Quinacrine 51% inhibition at 0.1 mg/ml Giardia intestinalis
Zn2+ 89% inhibition at 0.1 mg/ml Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 NADH at pH 7.8 and 25°C Giardia intestinalis
0.0874
-
 NADPH at pH 7.8 and 25°C Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
214000
-
 gel filtration Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + O2 Giardia intestinalis
-
 NAD+ + H2O
-
 ?
NADPH + H+ + O2 Giardia intestinalis
-
 NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Giardia intestinalis

Storage Stability

Storage Stability Organism
-70°C, 50% (v/v) glycerol, at least 2 months, no loss of activity Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + O2
-
 Giardia intestinalis NAD+ + H2O
-
 ?
NADPH + H+ + O2
-
 Giardia intestinalis NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 50000, SDS-PAGE Giardia intestinalis
homotetramer 4 * 50300, calculated from amino acid sequence Giardia intestinalis

Synonyms

Synonyms Comment Organism
NOX
-
 Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Giardia intestinalis

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains 0.91 mol FAD per mol of monomer Giardia intestinalis