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Literature summary for 1.6.2.4 extracted from
- Molecular cloning and functional characterization of NADPH-dependent cytochrome P450 reductase from the green microalga Botryococcus braunii, B race (2018), J. Biosci. Bioeng., 125, 30-37 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, truncation of the N-terminal transmembrane anchor and addition of a 6x His-tag | Botryococcus braunii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0094 | - |
NADPH | pH 7.5, 41°C | Botryococcus braunii |  |
| 0.0117 | - |
ferricytochrome c | pH 7.5, 41°C | Botryococcus braunii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Botryococcus braunii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, by Ni-NTA affinity chromatography | Botryococcus braunii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 80.3 | - |
pH 7.5, 41°C | Botryococcus braunii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH + H+ | - |
Botryococcus braunii | 2 ferrocytochrome c + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 71460, calculated from sequence, x * 75000, SDS-PAGE | Botryococcus braunii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | - |
Botryococcus braunii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.78 | - |
ferricytochrome c | pH 7.5, 41°C | Botryococcus braunii | |
| 3.66 | - |
NADPH | pH 7.5, 41°C | Botryococcus braunii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Botryococcus braunii |
| 7.7 | - |
assay at | Botryococcus braunii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Botryococcus braunii | calculated from sequence | - |
5.5 |
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