Information on EC 1.6.2.4 - NADPH-hemoprotein reductase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.6.2.4
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RECOMMENDED NAME
GeneOntology No.
NADPH-hemoprotein reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NADPH + H+ + n oxidized hemoprotein = NADP+ + n reduced hemoprotein
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
NADPH:hemoprotein oxidoreductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases (e.g. EC 1.14.14.1, unspecific monooxygenase) by stabilizing the one-electron reduced form of the flavin cofactors FAD and FMN. It also reduces cytochrome b5 and cytochrome c. The number n in the equation is 1 if the hemoprotein undergoes a 2-electron reduction, and is 2 if it undergoes a 1-electron reduction.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-03-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene cpr
UniProt
Manually annotated by BRENDA team
gene cpr
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
grown on alkanes or glycerol
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Manually annotated by BRENDA team
gene CsCPR
UniProt
Manually annotated by BRENDA team
Corbula caribea
significant activity only in organisms isolated from oil seep site, very low activity in organisms from control site
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Manually annotated by BRENDA team
e.g. maize, potato, avocado, bramble, tulip, leek, Vicia faba, sunflower, bramble, Gingko biloba
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
NMRI/Kisslegg strain
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
L. cv Dobrujanski 7
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
diamondback moth
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Saccharomyces cerevisiae /Homo sapiens
chimeric protein
Uniprot
Manually annotated by BRENDA team
JL20 strain
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
southern armyworm
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
carmine spider mite
UniProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricyanide + NADPH
2 ferrocyanide + NADP+ + H+
show the reaction diagram
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
2 ferricytochrome c + NADPH
2 ferrocytochrome c + NADP+ + H+
show the reaction diagram
2 ferricytochrome P450 + NADPH
2 ferrocytochrome P450 + NADP+ + H+
show the reaction diagram
2 oxidized cytochrome c + NADPH + H+
2 reduced cytochrome c + NADP+
show the reaction diagram
2,5-diaziridinyl-3-(hydroxymethyl)-6-methyl-1,4-benzoquinone + NADPH
NADP+ + ?
show the reaction diagram
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i.e. RH1, the enzyme reduces the antitumor drug RH1 to a semiquinone free radical. Following this reduction RH1 undergoes redox cycling under oxic conditions to produce potentially damaging hydroxyl radicals
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?
benzphetamine
?
show the reaction diagram
cocaine
?
show the reaction diagram
ferricytochrome c + dithionite
ferrocytochrome c + ?
show the reaction diagram
NADH + H+ + ferricytochrome c
NAD+ + ferricytochrome c
show the reaction diagram
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very low activity with NADH
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?
NADPH + 5-cyano-2,3-di-p-tolyltetrazolium chloride
NADP+ + (5-cyano-2,3-di-p-tolyltetrazolium chloride) formazan
show the reaction diagram
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formazan is fluorescent
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?
NADPH + cytochrome P-450
reduced cytochrome P-450 + NADP+
show the reaction diagram
NADPH + H+ + 1-[3-(4-phenoxyphenoxy)-2-oxopropyl]indole-5-carboxylic acid
NADP+ + 1-[2-hydroxy-3-(4-phenoxyphenoxy)propyl]indole-5-carboxylic acid
show the reaction diagram
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?
NADPH + H+ + 2 oxidized cytochrome c
NADP+ + 2 reduced cytochrome c
show the reaction diagram
NADPH + H+ + 2 oxidized cytochrome P450 2B4
NADP+ + 2 reduced cytochrome P450 2B4
show the reaction diagram
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?
NADPH + H+ + 2,4,6-trinitrotoluene
NADP+ + nitrite + ?
show the reaction diagram
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?
NADPH + H+ + 4-nitroso-2,6-dinitrotoluene
NADP+ + 4-hydroxyamino-2,6-dinitrotoluene
show the reaction diagram
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?
NADPH + H+ + cytochrome c
NADP+ + reduced cytochrome c
show the reaction diagram
NADPH + H+ + daunorubicin
NADP+ + ?
show the reaction diagram
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?
NADPH + H+ + dichlorophenolindophenol
NADP+ + reduced dichlorophenolindophenol
show the reaction diagram
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?
NADPH + H+ + doxorubicin
NADP+ + 7-deoxydoxorubicinone
show the reaction diagram
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?
NADPH + H+ + ferricyanide
NADP+ + ferrocyanide
show the reaction diagram
NADPH + H+ + ferricytochrome b5
NADP+ + ferrocytochrome b5
show the reaction diagram
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?
NADPH + H+ + ferricytochrome c
NADP+ + ferrocytochrome c
show the reaction diagram
NADPH + H+ + ferrocytochrome b5
NADP+ + ferricytochrome b5
show the reaction diagram
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?
NADPH + H+ + haloperidol
NADP+ + ?
show the reaction diagram
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?
NADPH + H+ + n oxidized hemoprotein
NADP+ + n reduced hemoprotein
show the reaction diagram
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
NADP+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
NADPH + H+ + oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
NADP+ + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
show the reaction diagram
NADPH + H+ + oxidized 5-cyano-2,3-ditolyl tetrazolium chloride
NADP+ + reduced 5-cyano-2,3-ditolyl tetrazolium chloride
show the reaction diagram
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?
NADPH + H+ + oxidized CYP106A1
NADP+ + reduced CYP106A1
show the reaction diagram
NADPH + H+ + oxidized CYP21A2
NADP+ + reduced CYP21A2
show the reaction diagram
NADPH + H+ + oxidized cytochrome c
NADP+ + reduced cytochrome c
show the reaction diagram
NADPH + H+ + oxidized eburicol
NADP+ + reduced eburicol
show the reaction diagram
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?
NADPH + H+ + oxidized lanosterol
NADP+ + reduced lanosterol
show the reaction diagram
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?
NADPH + H+ + oxidized menadione
NADP+ + reduced menadione
show the reaction diagram
NADPH + H+ + oxidized tetrazolium
NADP+ + reduced tetrazolium
show the reaction diagram
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?
NADPH + H+ + oxidized thiazolyl blue tetrazolium bromide
NADP+ + reduced thiazolyl blue tetrazolium bromide
show the reaction diagram
NADPH + hexadecanal
NADP+ + hexadecanol
show the reaction diagram
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brain, hexadecanal replaceable by p-nitroacetophenone, or p-pyridinecarboxaldehyde, benzalacetone or p-nitrobenzaldehyde
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?
NADPH + mitoxanthrone
NADP+ + ?
show the reaction diagram
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?
NADPH + O2
NADP+ + O2-
show the reaction diagram
NADPH + octanal
NADP+ + octanol
show the reaction diagram
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?
paraquat + NADPH
?
show the reaction diagram
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paraquat is a toxic herbicide
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome c + NADPH
2 ferrocytochrome c + NADP+ + H+
show the reaction diagram
2 ferricytochrome P450 + NADPH
2 ferrocytochrome P450 + NADP+ + H+
show the reaction diagram
2,5-diaziridinyl-3-(hydroxymethyl)-6-methyl-1,4-benzoquinone + NADPH
NADP+ + ?
show the reaction diagram
-
i.e. RH1, the enzyme reduces the antitumor drug RH1 to a semiquinone free radical. Following this reduction RH1 undergoes redox cycling under oxic conditions to produce potentially damaging hydroxyl radicals
-
-
?
NADPH + cytochrome P-450
reduced cytochrome P-450 + NADP+
show the reaction diagram
NADPH + H+ + cytochrome c
NADP+ + reduced cytochrome c
show the reaction diagram
NADPH + H+ + n oxidized hemoprotein
NADP+ + n reduced hemoprotein
show the reaction diagram
paraquat + NADPH
?
show the reaction diagram
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paraquat is a toxic herbicide
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?
additional information
?
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