Cloned (Comment) | Organism |
---|---|
expression of cytosolic isozyme AtCKX7 in Escherichia coli and intracellularly in Pichia pastoris | Arabidopsis thaliana |
expression of vacuolar His-tagged isozyme AtCKX1 in Pichia pastoris | Arabidopsis thaliana |
expression of vacuolar isozyme AtCKX3 in Pichia pastoris | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apoplast | secreted isozymes | Arabidopsis thaliana | 48046 | - |
cytosol | isozyme AtCKX7 | Arabidopsis thaliana | 5829 | - |
vacuole | isozyme AtCKX1 | Arabidopsis thaliana | 5773 | - |
vacuole | isozyme AtCKX3 | Arabidopsis thaliana | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57057 | - |
x * 60000, SDS-PAGE, x * 57057, mass spectrometry | Arabidopsis thaliana |
58080 | - |
x * 92000, SDS-PAGE, x * 59720, sequence calculation, x * 58080, mass spectrometry | Arabidopsis thaliana |
59720 | - |
x * 92000, SDS-PAGE, x * 59720, sequence calculation, x * 58080, mass spectrometry | Arabidopsis thaliana |
60000 | - |
x * 60000, SDS-PAGE, x * 57057, mass spectrometry | Arabidopsis thaliana |
64290 | - |
x * 97000, SDS-PAGE, x * 65050, sequence calculation, x * 64290, mass spectrometry | Arabidopsis thaliana |
65050 | - |
x * 97000, SDS-PAGE, x * 65050, sequence calculation, x * 64290, mass spectrometry | Arabidopsis thaliana |
92000 | - |
x * 92000, SDS-PAGE, x * 59720, sequence calculation, x * 58080, mass spectrometry | Arabidopsis thaliana |
97000 | - |
x * 97000, SDS-PAGE, x * 65050, sequence calculation, x * 64290, mass spectrometry | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isozyme AtCKX7 | - |
Arabidopsis thaliana | O22213 | isozyme AtCKX1 | - |
Arabidopsis thaliana | Q9LTS3 | isozyme AtCKX3 | - |
Purification (Comment) | Organism |
---|---|
recombinant vacuolar His-tagged isozyme AtCKX1 41.7fold from Pichia pastoris by ultrafiltration, hydrophobic interaction and hydroxyapatite chromatography, followed by nickel affinity chromatography and another step of hydrophobic interaction chromatography | Arabidopsis thaliana |
recombinant vacuolar isozyme AtCKX3 18fold from Pichia pastoris by ultrafiltration, hydroxyapatite chromatography, nickel affinity chromatography, and anion exchange chromatography and another step of hydrophobic interaction chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kinetin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
kinetin riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
additional information | acceptor and substrate specificity, overview. The enzyme catalyzes the irreversible cleavage of N6-side chains from cytokinins. CKXs also show low cytokinin oxidase activity, but molecular oxygen is a comparatively poor electron acceptor. Vacuolar AtCKX enzymes in certain conditions degrade N6-(2-isopentenyl)adenine di- and triphosphates two to 5times more effectively than its monophosphate | Arabidopsis thaliana | ? | - |
? | |
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O | - |
Arabidopsis thaliana | adenine + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-glucoside + 3',5'-dimethoxy-4'-hydroxyacetophenone + H2O | i.e. iP9G, best substrate for isozyme AtCKX1 at pH 5.0, 3',5'-dimethoxy-4-hydroxyacetophenone is the best acceptor | Arabidopsis thaliana | adenine 9-beta-D-glucoside + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-riboside + 2,3,5-triphenyl-tetrazolium chloride + H2O | - |
Arabidopsis thaliana | adenine 9-beta-D-riboside + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O | - |
Arabidopsis thaliana | adenine 9-beta-D-riboside + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-riboside + 3',5'-dimethoxy-4'-hydroxyacetophenone + H2O | - |
Arabidopsis thaliana | adenine 9-beta-D-riboside + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-riboside + ferricyanide + H2O | - |
Arabidopsis thaliana | adenine 9-beta-D-riboside + 3-methylbut-2-enal + ferrocyanide | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-riboside + ferricytochrome c + H2O | - |
Arabidopsis thaliana | adenine 9-beta-D-riboside + 3-methylbut-2-enal + ferrocytochrome c | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-riboside + NAD+ + H2O | - |
Arabidopsis thaliana | adenine 9-beta-D-riboside + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-riboside + O2 + H2O | - |
Arabidopsis thaliana | adenine 9-beta-D-riboside + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-riboside + oxidized 2,6-dichlorophenolindophenol + H2O | - |
Arabidopsis thaliana | adenine 9-beta-D-riboside + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol | - |
? | |
N6-(2-isopentenyl)adenine 9-beta-D-riboside 5'-phosphate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O | - |
Arabidopsis thaliana | adenine 9-beta-D-riboside 5'-phosphate + 3-methylbut-2-enal + ? | - |
? | |
trans-zeatin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O | - |
Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE, x * 57057, mass spectrometry | Arabidopsis thaliana |
? | x * 92000, SDS-PAGE, x * 59720, sequence calculation, x * 58080, mass spectrometry | Arabidopsis thaliana |
? | x * 97000, SDS-PAGE, x * 65050, sequence calculation, x * 64290, mass spectrometry | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtCKX3 | - |
Arabidopsis thaliana |
CKX | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoprotein | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the CKX gene family of Arabidopsis thaliana comprises seven members | Arabidopsis thaliana |