Crystallization (Comment) | Organism |
---|---|
crystal structure of the recombinant mature and precursor forms of enzyme and the enzyme complexed with 5,6,7,8-tetrahydrofolate. Both forms reveal similar kinetic parameters and have the same tertiary structure fold with two domains formed by N- and C-terminal halves of the protein. The active center is located in the N-terminal domain while the tetrahydrofolate binding site is located in the C-terminal domain about 40 A from the isoalloxazine ring of FAD. The folate binding site is connected with the enzyme active center via an intramolecular channel. This suggests the possible transfer of the intermediate imine of dimethylglycine from the active center to the bound 5,6,7,8-tetrahydrofolate where they can react producing a 5,10-methylenetetrahydrofolate | Rattus norvegicus |
recombinant mature and precursor forms, in complex with tetrahydrofolate. Both forms of DMGDH reveal similar kinetic parameters and have the same tertiary structure fold with two domains formed by N- and C-terminal halves of the protein. The active center is located in the N-terminal domain while the THF binding site is located in the C-terminal domain about 40 A from the isoalloxazine ring of FAD. The folate binding site is connected with the enzyme active center via an intramolecular channel | Rattus norvegicus |
using 0.2 M K/Na-tartrate-20% (w/v) PEG 3350 for the mature enzyme or 0.2 M NaSCN-20% (w/v) PEG 3350-0.1 M Tris pH 7.5 for the precursor | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylglycine + electron-transfer flavoprotein + H2O | Rattus norvegicus | - |
sarcosine + formaldehyde + reduced electron-transfer flavoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus | Q5RKL4 | - |
- |
Rattus norvegicus | Q63342 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylglycine + electron-transfer flavoprotein + H2O | - |
Rattus norvegicus | sarcosine + formaldehyde + reduced electron-transfer flavoprotein | - |
? | |
N,N-dimethylglycine + oxidized ferricenium hexafluorophosphate + H2O | - |
Rattus norvegicus | sarcosine + formaldehyde + reduced ferricenium hexafluorophosphate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 96000, calculated from amino acid sequence | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
DMGDH | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus | |
tetrahydrofolate | - |
Rattus norvegicus |