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Information on EC 1.5.8.4 - dimethylglycine dehydrogenase
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EC Tree
1.5.8.4 dimethylglycine dehydrogenaseIUBMB Comments
A flavoprotein, containing a histidyl(Npi)-(8alpha)FAD linkage at position 91 in the human protein. An imine intermediate is channeled from the FAD binding site to the 5,6,7,8-tetrahydrofolate binding site through a 40 A tunnel [5,8,9]. In the absence of 5,6,7,8-tetrahydrofolate the enzyme forms formaldehyde [5,9].
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
dimethylglycine dehydrogenase, dmgdh, me2glydh, csal_0990, n,n-dimethylglycine oxidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Csal_0990
DdhC
DMGDH
EC 1.5.99.2
-
-
formerly
-
Me2GlyDH
N,N-dimethylglycine oxidase
-
-
-
-
Me2GlyDH
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N,N-dimethylglycine + 5,6,7,8-tetrahydrofolate + electron-transfer flavoprotein = sarcosine + 5,10-methylenetetrahydrofolate + reduced electron-transfer flavoprotein
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
oxidative deamination
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
N,N-dimethylglycine,5,6,7,8-tetrahydrofolate:electron-transferflavoprotein oxidoreductase (demethylating,5,10-methylenetetrahydrofolate-forming)
A flavoprotein, containing a histidyl(Npi)-(8alpha)FAD linkage at position 91 in the human protein. An imine intermediate is channeled from the FAD binding site to the 5,6,7,8-tetrahydrofolate binding site through a 40 A tunnel [5,8,9]. In the absence of 5,6,7,8-tetrahydrofolate the enzyme forms formaldehyde [5,9].
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CAS REGISTRY NUMBER
COMMENTARY
37256-30-7
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5,10-methenyltetrahydrofolate + FADH2
5,10-methylene tetrahydrofolate + FAD
-
-
-
-
?
epsilon-N-methyl-L-lysine + acceptor + H2O
formaldehyde + L-lysine + reduced acceptor
-
-
-
?
N,N-dimethylglycine + 5,6,7,8-tetrahydrofolate + electron-transfer flavoprotein
sarcosine + 5,10-methylenetetrahydrofolate + reduced electron-transfer flavoprotein
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
sarcosine + formaldehyde + reduced electron-transfer flavoprotein
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
sarcosine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
N,N-dimethylglycine + ferricenium + H2O
sarcosine + formaldehyde + ferrocene
-
-
-
?
N,N-dimethylglycine + ferricenium hexafluorophosphate + H2O
sarcosine + formaldehyde + reduced ferricenium hexafluorophosphate
-
-
-
-
?
N,N-dimethylglycine + H2O + ferrocene
sarcosine + formaldehyde + ?
-
-
-
?
N,N-dimethylglycine + H2O + oxidized 1-methoxy-5-methylphenazinium methylsulfate
sarcosine + formaldehyde + reduced 1-methoxy-5-methylphenazinium methylsulfate
-
-
-
-
?
N,N-dimethylglycine + H2O + oxidized 2,6-dichlorophenolindophenol
sarcosine + formaldehyde + reduced 2,6-dichlorophenolindophenol
N,N-dimethylglycine + oxidized ferricenium hexafluorophosphate + H2O
sarcosine + formaldehyde + reduced ferricenium hexafluorophosphate + H2O2
-
-
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
N-methyl-L-alanine + acceptor + H2O
formaldehyde + L-alanine + reduced acceptor
-
-
-
?
sarcosine + acceptor + H2O
glycine + formaldehyde + reduced acceptor
-
-
-
?
sarcosine + electron-transfer flavoprotein + H2O
glycine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
N,N-dimethylglycine + 5,6,7,8-tetrahydrofolate + electron-transfer flavoprotein
sarcosine + 5,10-methylenetetrahydrofolate + reduced electron-transfer flavoprotein
the enzyme takes part in choline degradation, one-carbon metabolism and electron transfer to the respiratory chain
-
-
?
N,N-dimethylglycine + 5,6,7,8-tetrahydrofolate + electron-transfer flavoprotein
sarcosine + 5,10-methylenetetrahydrofolate + reduced electron-transfer flavoprotein
ferrocene or 2,6-dichlorophenolindophenol are used as artificial electron acceptor
-
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
-
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
bound tetrahydrofolate interacts directly with the enzyme and serves as an acceptor for the one-carbon unit produced during the oxidative demethylation of dimethylglycine
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + acceptor + H2O
sarcosine + formaldehyde + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
-
?
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
sarcosine + formaldehyde + reduced electron-transfer flavoprotein
-
-
-
?
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
sarcosine + formaldehyde + reduced electron-transfer flavoprotein
-
-
-
?
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
sarcosine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
-
-
r
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
sarcosine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
-
-
r
N,N-dimethylglycine + H2O + oxidized 2,6-dichlorophenolindophenol
sarcosine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
?
N,N-dimethylglycine + H2O + oxidized 2,6-dichlorophenolindophenol
sarcosine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
tetrahydropteroylpentaglutamate is used in vivo as an acceptor of the one-carbon group generated during the reaction
-
?
sarcosine + electron-transfer flavoprotein + H2O
glycine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
-
-
r
sarcosine + electron-transfer flavoprotein + H2O
glycine + formaldehyde + reduced electron-transfer flavoprotein + H2O2
-
-
-
r
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5,10-methenyltetrahydrofolate + FADH2
5,10-methylene tetrahydrofolate + FAD
-
-
-
-
?
N,N-dimethylglycine + 5,6,7,8-tetrahydrofolate + electron-transfer flavoprotein
sarcosine + 5,10-methylenetetrahydrofolate + reduced electron-transfer flavoprotein
the enzyme takes part in choline degradation, one-carbon metabolism and electron transfer to the respiratory chain
-
-
?
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
sarcosine + formaldehyde + reduced electron-transfer flavoprotein
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
sarcosine + formaldehyde + reduced electron-transfer flavoprotein
-
-
-
?
N,N-dimethylglycine + electron-transfer flavoprotein + H2O
sarcosine + formaldehyde + reduced electron-transfer flavoprotein
-
-
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
during the reaction tightly bound tetrahydropteroylpentaglutamate is converted to 5,10-methylene tetrahydropteroylpentaglutamate
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
reaction of the choline degradation pathway
-
?
N,N-dimethylglycine + tetrahydropteroylpentaglutamate + acceptor
sarcosine + 5,10-methylene tetrahydropteroylpentaglutamate + reduced acceptor
-
tetrahydropteroylpentaglutamate is used in vivo as an acceptor of the one-carbon group generated during the reaction
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
-
covalently bound
FAD
-
covalent attachment of FAD to the apoenzyme proceeds in vitro spontaneously and does not require a mitochondrial protein factor
FAD
-
the precursor form of the enzyme is a good substrate for mitochondria-assisted flavinylation reaction, flavinylation of precursor precedes polypeptide processing by the mitochondrial processing peptidase during the biogenesis of holo-enzyme
FAD
-
mitochondrial matrix can stimulate the flavinylation of the enzyme synthesized in rabbit reticulocyte lysate as well as the 6-His-tagged enzyme purified by affinity chromatography, a matrix protein factor accelerates holoenzyme formation of dimethylglycine dehydrogenase, the matrix protein factor is different from the mitochondrial chaperones Hsp60 and Hsp 70
FAD
-
absolutely required, covalently bound to the apoenzyme via a histidinyl(N3)-(8alpha)FAD linkage, cofactor-free apoenzyme shows 70% reduced activity compared to the holoenzyme
FAD
A280/A450 ratios of the highly pure protein fractions are between 14-16 and 20-25 for wild-type and mutant H109R, respectively. The redox potential for the reduction of FAD is -93 mV
FAD
noncovalently bound flavin adenine dinucleotide, purified protein shows the absorption spectrum of an oxidized flavoprotein, with maxima at 222, 275, 370, and 440 nm and one shoulder at around 470 nm
additional information
-
1-methoxy-5-methylphenazinium methylsulfate can act as electron acceptor
-
additional information
-
2,6-dichlorophenolindophenol can act as electron acceptor
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Nonheme iron
additional information
Na+, K+, Ca2+, Zn2+, Mn2+, Mg2+, Fe2+, and Co2+ display no or slightly inhibitory effects at 2 mM
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
Na+, K+, Ca2+, Zn2+, Mn2+, Mg2+, Fe2+, and Co2+ display no or slightly inhibitory effects at 2 mM
-
additional information
the expressions of 23 genes, including the dimethylglycine dehydrogenase precursor pMe2GlyDH, are significantly down-regulated in the hearts of cardio-specific caspase recruitment domain transgenic mice. Caspase recruitment domain ARC may protect cardiomyocytes from hypoxic cell death by regulating its downstream, dimethylglycine dehydrogenase and developmentally regulated RNA-binding protein Drbp1
-
additional information
-
the expressions of 23 genes, including the dimethylglycine dehydrogenase precursor pMe2GlyDH, are significantly down-regulated in the hearts of cardio-specific caspase recruitment domain transgenic mice. Caspase recruitment domain ARC may protect cardiomyocytes from hypoxic cell death by regulating its downstream, dimethylglycine dehydrogenase and developmentally regulated RNA-binding protein Drbp1
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Carcinoma, Hepatocellular
LncRNA RP11-422N16.3 Inhibits Cell Proliferation and EMT, and Induces Apoptosis in Hepatocellular Carcinoma Cells by Sponging miR-23b-3p.
Carcinoma, Hepatocellular
Potential diagnostic and prognostic marker dimethylglycine dehydrogenase (DMGDH) suppresses hepatocellular carcinoma metastasis in vitro and in vivo.
Carcinoma, Hepatocellular
Rat liver dimethylglycine dehydrogenase. Flavinylation of the enzyme in hepatocytes in primary culture and characterization of a cDNA clone.
dimethylglycine dehydrogenase deficiency
Cloning of dimethylglycine dehydrogenase and a new human inborn error of metabolism, dimethylglycine dehydrogenase deficiency.
dimethylglycine dehydrogenase deficiency
Defect in dimethylglycine dehydrogenase, a new inborn error of metabolism: NMR spectroscopy study.
dimethylglycine dehydrogenase deficiency
Molecular basis of dimethylglycine dehydrogenase deficiency associated with pathogenic variant H109R.
dimethylglycine dehydrogenase deficiency
Structure and biochemical properties of recombinant human dimethylglycine dehydrogenase and comparison to the disease-related H109R variant.
dimethylglycine dehydrogenase deficiency
[DMGDH gene-related dimethylglycine dehydrogenase deficiency in a case].
Liver Neoplasms
LncRNA RP11-422N16.3 Inhibits Cell Proliferation and EMT, and Induces Apoptosis in Hepatocellular Carcinoma Cells by Sponging miR-23b-3p.
Neoplasm Metastasis
Potential diagnostic and prognostic marker dimethylglycine dehydrogenase (DMGDH) suppresses hepatocellular carcinoma metastasis in vitro and in vivo.
Neoplasms
LncRNA RP11-422N16.3 Inhibits Cell Proliferation and EMT, and Induces Apoptosis in Hepatocellular Carcinoma Cells by Sponging miR-23b-3p.
Non-alcoholic Fatty Liver Disease
Nonalcoholic Steatohepatitis is associated with a state of betaine-insufficiency.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.045
N,N-dimethylglycine
-
and second value 1.10 mM, wild-type, presence of tetrahydrofolate, pH 7.5, 25°C
0.05
N,N-dimethylglycine
-
pH 7.0, 30°C, recombinant holoenzyme, 2 Km-values
0.3
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: 2,6-dichlorophenolindophenol, wild-type enzyme
0.3
N,N-dimethylglycine
wild type enzyme, with 2,6-dichlorophenolindophenol as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
1.1
N,N-dimethylglycine
-
and first value 0.045 mM, wild-type, presence of tetrahydrofolate, pH 7.5, 25°C
1.4
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: ferrocene, wild-type enzyme
1.4
N,N-dimethylglycine
wild type enzyme, with ferrocene as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
4.7
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: 2,6-dichlorophenolindophenol, mutant enzyme H109R
4.7
N,N-dimethylglycine
mutant enzyme H109R, with 2,6-dichlorophenolindophenol as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
9.4
N,N-dimethylglycine
-
pH 7.0, 30°C, recombinant holoenzyme, 2 Km-values
32.2
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: ferrocene, mutant enzyme H109R
32.2
N,N-dimethylglycine
mutant enzyme H109R, with ferrocene as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.225
N,N-dimethylglycine
-
wild-type, presence of tetrahydrofolate, pH 7.5, 25°C
0.73
N,N-dimethylglycine
wild type enzyme, with 2,6-dichlorophenolindophenol as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
0.733
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: 2,6-dichlorophenolindophenol, wild-type enzyme
1.5
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: 2,6-dichlorophenolindophenol, mutant enzyme H109R
1.52
N,N-dimethylglycine
mutant enzyme H109R, with 2,6-dichlorophenolindophenol as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
3.55
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: ferrocene, wild-type enzyme
3.55
N,N-dimethylglycine
wild type enzyme, with ferrocene as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
9.07
N,N-dimethylglycine
mutant enzyme H109R, with ferrocene as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
9.1
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: ferrocene, mutant enzyme H109R
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.18
N,N-dimethylglycine
mutant enzyme H109R, with ferrocene as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
0.28
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: ferrocene, mutant enzyme H109R
0.32
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: 2,6-dichlorophenolindophenol, mutant enzyme H109R
0.33
N,N-dimethylglycine
mutant enzyme H109R, with 2,6-dichlorophenolindophenol as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
2.28
N,N-dimethylglycine
wild type enzyme, with ferrocene as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
2.43
N,N-dimethylglycine
wild type enzyme, with 2,6-dichlorophenolindophenol as cosubstrate, at 25°C and pH 7.8 in 50 mM HEPES/NaOH with 150 mM NaCl
2.44
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: 2,6-dichlorophenolindophenol, wild-type enzyme
2.54
N,N-dimethylglycine
25°C, pH 7.8, artificial electron acceptor: ferrocene, wild-type enzyme
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5 - 9
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
brenda
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