Literature summary for 1.5.3.5 extracted from

Li, J.; Shen, M.; Chen, Z.; Pan, F.; Yang, Y.; Shu, M.; Chen, G.; Jiao, Y.; Zhang, F.; Linhardt, R.; Zhong, W.
Expression and functional identification of two homologous nicotine dehydrogenases, NicA2 and Nox, from Pseudomonas sp. JY-Q (2021), Protein Expr. Purif., 178, 105767 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas sp. JY-Q

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.225	-	Nicotine	37°C, pH not specified in the publication	Pseudomonas sp. JY-Q
0.346	-	Nicotine	37°C, pH not specified in the publication	Pseudomonas sp. JY-Q

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. JY-Q	-	-	-
Pseudomonas sp. JY-Q	A0A0S4IEN1	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Pseudomonas sp. JY-Q

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nicotine + H2O + O2	-	Pseudomonas sp. JY-Q	N-methylmyosmine + H2O2	-	?

Subunits

Subunits	Comment	Organism
?	x * 55700, SDS-PAGE	Pseudomonas sp. JY-Q
?	x * 56100, SDS-PAGE	Pseudomonas sp. JY-Q

Synonyms

Synonyms	Comment	Organism
NicA2	-	Pseudomonas sp. JY-Q
NOX	-	Pseudomonas sp. JY-Q

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Pseudomonas sp. JY-Q
40	-	-	Pseudomonas sp. JY-Q

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	38% residual activity after 120 min	Pseudomonas sp. JY-Q
60	-	50% residual activity after 35 min	Pseudomonas sp. JY-Q

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	7.5	-	Pseudomonas sp. JY-Q
7.5	-	-	Pseudomonas sp. JY-Q

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Pseudomonas sp. JY-Q
NAD+	-	Pseudomonas sp. JY-Q

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.018	-	Nicotine	37°C, pH not specified in the publication	Pseudomonas sp. JY-Q
0.024	-	Nicotine	37°C, pH not specified in the publication	Pseudomonas sp. JY-Q

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Release 2023.1 (January 2023)