BRENDA - Enzyme Database
show all sequences of 1.5.1.31

Formation of (R)-configurated tetrahydroberberine alkaloids in vivo and in vitro

Bauer, W.; Zenk, M.H.; Tetrahedron Lett. 32, 487-490 (1991)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
berberine + NADPH
Corydalis cava
(R)-tetrahydroberberines are formed from protoberberines by a highly stereoselective reduction system
canadine + NADP+
-
Corydalis cava
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corydalis cava
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
bulb
-
Corydalis cava
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7,8-dihydroberberine + NADPH
at 64% of the activity with palmatine and NADPH
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
berberine + NADH
at 59% of the activity with palmatine. At 15% of the activity with berberine and NADPH
440357
Corydalis cava
canadine + NAD+
-
440357
Corydalis cava
r
berberine + NADPH
at 59% of the activity with palmatine and NADPH
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
berberine + NADPH
(R)-tetrahydroberberines are formed from protoberberines by a highly stereoselective reduction system
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
columbamine + NADPH
at 39% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
coptisine + NADPH
at 47% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
dehydroscoulerine + NADPH
at 5% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
-
-
r
dihydrosinactine + NADPH
at 52% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
jatrorrhizine + NADPH
at 25% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
-
-
r
palmatine + NADPH
-
440357
Corydalis cava
? + NADP+
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Corydalis cava
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Corydalis cava
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
at 15% of the activity with NADPH
Corydalis cava
NADP+
-
Corydalis cava
NADPH
-
Corydalis cava
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
at 15% of the activity with NADPH
Corydalis cava
NADP+
-
Corydalis cava
NADPH
-
Corydalis cava
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
berberine + NADPH
Corydalis cava
(R)-tetrahydroberberines are formed from protoberberines by a highly stereoselective reduction system
canadine + NADP+
-
Corydalis cava
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
bulb
-
Corydalis cava
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7,8-dihydroberberine + NADPH
at 64% of the activity with palmatine and NADPH
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
berberine + NADH
at 59% of the activity with palmatine. At 15% of the activity with berberine and NADPH
440357
Corydalis cava
canadine + NAD+
-
440357
Corydalis cava
r
berberine + NADPH
at 59% of the activity with palmatine and NADPH
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
berberine + NADPH
(R)-tetrahydroberberines are formed from protoberberines by a highly stereoselective reduction system
440357
Corydalis cava
canadine + NADP+
-
440357
Corydalis cava
r
columbamine + NADPH
at 39% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
coptisine + NADPH
at 47% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
dehydroscoulerine + NADPH
at 5% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
-
-
r
dihydrosinactine + NADPH
at 52% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
440357
Corydalis cava
r
jatrorrhizine + NADPH
at 25% of the activity with palmatine and NADPH
440357
Corydalis cava
? + NADP+
-
-
-
r
palmatine + NADPH
-
440357
Corydalis cava
? + NADP+
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Corydalis cava
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Corydalis cava
Other publictions for EC 1.5.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743234
Galanie
Optimization of yeast-based p ...
Berberis wilsoniae
Microb. Cell Fact.
14
144
2015
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1
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1
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1
1
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1
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1
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440357
Bauer
-
Formation of (R)-configurated ...
Corydalis cava
Tetrahedron Lett.
32
487-490
1991
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1
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1
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10
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1
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1
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3
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3
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1
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1
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10
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1
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1
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