General Stability | Organism |
---|---|
glycerol stabilizes | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | - |
Streptomyces coelicolor | |
L-valine | substrate inhibition at higher concentrations than 20 mM | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.093 | - |
NADH | - |
Streptomyces coelicolor | |
0.17 | - |
NAD+ | - |
Streptomyces coelicolor | |
0.6 | - |
2-oxoisovalerate | - |
Streptomyces coelicolor | |
10 | - |
L-valine | - |
Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Streptomyces coelicolor |
70000 | - |
gel filtration | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-valine + NAD(P)+ + H2O | Streptomyces coelicolor | metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
L-valine + NAD(P)+ + H2O | Streptomyces coelicolor | first step in valine catabolism | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
143fold purification | Streptomyces coelicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
L-alanine | Streptomyces coelicolor |
1.2 | - |
L-leucine | Streptomyces coelicolor |
3.8 | - |
L-isoleucine | Streptomyces coelicolor |
14 | - |
L-valine | Streptomyces coelicolor |
19 | - |
L-2-aminobutyrate | Streptomyces coelicolor |
Storage Stability | Organism |
---|---|
-80°C, 50 mM potassium phosphate pH 7.5, 10% v/v glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, 1 month, loss of specific activity | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminobutyrate + H2O + NAD+ | 130% of the activity with L-valine | Streptomyces coelicolor | 2-oxobutyrate + NADH + NH3 | - |
? | |
L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Streptomyces coelicolor | 2-oxobutyrate + NADH + NH3 | - |
? | |
L-alanine + H2O + NAD+ | 5% of the activity with L-valine | Streptomyces coelicolor | pyruvate + NH3 + NADH | - |
r | |
L-cysteine + H2O + NAD+ | 3% of the activity with L-valine | Streptomyces coelicolor | 3-mercapto-2-oxopropanoate + NH3 + NADH | - |
? | |
L-isoleucine + H2O + NAD+ | 28% of the activity with L-valine | Streptomyces coelicolor | 3-methyl-2-oxopentanoate + NADH + NH3 | - |
? | |
L-leucine + H2O + NAD+ | 8% of the activity with L-valine | Streptomyces coelicolor | 2-oxoisocaproate + NADH + NH3 | - |
? | |
L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces coelicolor | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces coelicolor | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | L-valine oxidized efficiently | Streptomyces coelicolor | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
L-valine + NAD(P)+ + H2O | L-valine oxidized efficiently | Streptomyces coelicolor | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics | Streptomyces coelicolor | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
L-valine + NAD(P)+ + H2O | first step in valine catabolism | Streptomyces coelicolor | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces coelicolor A3(2) | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces coelicolor A3(2) | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | L-valine oxidized efficiently | Streptomyces coelicolor A3(2) | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
L-valine + NAD(P)+ + H2O | L-valine oxidized efficiently | Streptomyces coelicolor A3(2) | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
additional information | not: D-valine | Streptomyces coelicolor | additional information | - |
? | |
additional information | not: D-valine | Streptomyces coelicolor A3(2) | additional information | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
reductive amination of 2-oxoisovalerate | Streptomyces coelicolor |
10.5 | - |
oxidative deamination of L-valine | Streptomyces coelicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | pH 8.5: 40% loss of activity, pH 9.5: 10% loss of activity | Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | not with NADP+ and NADPH | Streptomyces coelicolor | |
NAD+ | NAD+ required, no use of NADP+ | Streptomyces coelicolor | |
NADH | NADH could not be replaced with NADPH for the reductive amination | Streptomyces coelicolor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
36.8 | - |
L-valine | - |
Streptomyces coelicolor |