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Information on EC 1.4.1.8 - valine dehydrogenase (NADP+)
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EC Tree
1.4.1.8 valine dehydrogenase (NADP+)Specify your search results
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
valdh, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
dehydrogenase, valine (nicotinamide adenine dinucleotide phosphate)
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ValDH
valine dehydrogenase (nicotinamide adenine dinucleotide phosphate)
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VDH
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ValDH
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-valine + H2O + NADP+ = 3-methyl-2-oxobutanoate + NH3 + NADPH + H+
kinetic mechanism
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L-valine + H2O + NADP+ = 3-methyl-2-oxobutanoate + NH3 + NADPH + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
amination
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Deamination
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oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
L-valine:NADP+ oxidoreductase (deaminating)
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CAS REGISTRY NUMBER
COMMENTARY
37255-39-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-methionine + H2O + NAD+
4-(methylthio)-2-oxobutanoic acid + NH3 + NADH
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VDH2: 2% of the activity with L-valine
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-
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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VDH2: 125% of the activity with L-valine
VDH2: reductive amination, 116% of the activity with 2-oxoisovalerate, 2-oxobutyrate is identical with alpha-ketobutyrate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
L-2-aminobutyrate is identical with L-alpha-aminobutyrate
VDH2: reductive amination, 116% of the activity with 2-oxoisovalerate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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L-2-aminobutyrate is identical with L-alpha-aminobutyrate
2-oxobutyrate is identical with alpha-ketobutyrate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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L-2-aminobutyrate is identical with L-alpha-aminobutyrate
2-oxobutyrate is identical with alpha-ketobutyrate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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9% of the activity with L-leucine
reductive amination: 28.1% of the activity with 2-oxoisovalerate
?, r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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9% of the activity with L-leucine
2-oxobutyrate is identical with alpha-ketobutyrate
?, r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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L-2-aminobutyrate is identical with L-alpha-aminobutyrate
reductive amination: 28.1% of the activity with 2-oxoisovalerate
?, r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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L-2-aminobutyrate is identical with L-alpha-aminobutyrate
2-oxobutyrate is identical with alpha-ketobutyrate
?, r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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preferred substrate, 92.34% of the activity with L-valine
2-oxobutyrate is identical with alpha-ketobutyrate, reductive amination: 91.8% of the activity with 2-oxoisovalerate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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L-2-aminobutyrate is identical with L-alpha-aminobutyrate
2-oxobutyrate is identical with alpha-ketobutyrate, reductive amination: 91.8% of the activity with 2-oxoisovalerate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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130% of the activity with L-valine
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?
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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L-2-aminobutyrate is identical with L-alpha-aminobutyrate
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?
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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VDH2: preferred substrate, 158% of the activity with L-valine
VDH2: reductive amination, 100% of the activity with 2-oxoisovalerate, 2-oxobutyrate is identical with alpha-ketobutyrate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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68.9% of the activity with L-valine
reductive amination: 78% of the activity with 2-oxoisovalerate, 2-oxobutyrate is identical with alpha-ketobutyrate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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L-2-aminobutyrate is identical with L-alpha-aminobutyrate
reductive amination: 78% of the activity with 2-oxoisovalerate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
L-2-aminobutyrate is identical with L-alpha-aminobutyrate
VDH2: reductive amination, 100% of the activity with 2-oxoisovalerate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
L-2-aminobutyrate is identical with L-alpha-aminobutyrate
2-oxobutyrate is identical with alpha-ketobutyrate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
L-2-aminobutyrate is identical with L-alpha-aminobutyrate
2-oxobutyrate is identical with alpha-ketobutyrate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
L-2-aminobutyrate is identical with L-alpha-aminobutyrate
2-oxobutyrate is identical with alpha-ketobutyrate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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L-2-aminobutyrate is identical with L-alpha-aminobutyrate
2-oxobutyrate is identical with alpha-ketobutyrate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
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L-2-aminobutyrate is identical with L-alpha-aminobutyrate
2-oxobutyrate is identical with alpha-ketobutyrate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
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VDH2: 4.5% of the activity with L-valine
pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate, VDH2: reductive amination, 20.8% of the activity with 2-oxoisovalerate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
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3% of the activity with L-valine
reductive amination: 2% of the activity with 2-oxoisovalerate, pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
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13.63% of the activity with L-valine
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r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
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5% of the activity with L-valine
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r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
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14.7% of the activity with L-valine
pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate, reductive amination: 31.6% of the activity with 2-oxoisovalerate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
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VDH2: 8.3% of the activity with L-valine
pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate, VDH2: reductive amination, 23.3% of the activity with 2-oxoisovalerate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
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pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate
?
L-cysteine + H2O + NAD+
3-mercapto-2-oxopropanoate + NH3 + NADH
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1.87% of the activity with L-valine
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r
L-cysteine + H2O + NAD+
3-mercapto-2-oxopropanoate + NH3 + NADH
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3.75% of the activity with L-valine, A124G mutant: 0.063%
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r
L-cysteine + H2O + NAD+
3-mercapto-2-oxopropanoate + NH3 + NADH
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?
L-cysteine + H2O + NAD+
3-mercapto-2-oxopropanoate + NH3 + NADH
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3% of the activity with L-valine
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?
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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VDH2: 34% of the activity with L-valine
2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate, VDH2: reductive amination, 24.5% of the activity with 2-oxoisovalerate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate
?
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate
?
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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reductive amination: 59% of the activity of 2-oxoisovalerate, but no oxidative deamination of L-isoleucine
?
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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reductive amination: 59% of the activity of 2-oxoisovalerate, but no oxidative deamination of L-isoleucine
?
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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61.3% of the activity with L-leucine
2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate, reductive amination: 21.9% of the activity with 2-oxoisovalerate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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27.5% of the activity with L-valine, A124G mutant: 9.98%
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r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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12.46% of the activity with L-valine
2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate, reductive amination: 35.6% of the activity with 2-oxoisovalerate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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27.5% of the activity with L-valine
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?
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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28% of the activity with L-valine
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?
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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VDH2: 22% of the activity with L-valine
2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate, VDH2: reductive amination, 16.6% of the activity with 2-oxoisovalerate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
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29.4% of the activity with L-valine
2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate, reductive amination: 23.2% of the activity with 2-oxoisovalerate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
-
-
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r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
-
-
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
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VDH2: 52.8% of the activity with L-valine
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate, VDH2: reductive amination, 26.5% of the activity with 2-oxoisovalerate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
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preferred substrate
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate, reductive amination: 21.1% of the activity with 2-oxoisovalerate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
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7.46% of the activity with L-valine
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate, reductive amination: 26.85% of the activity with 2-oxoisovalerate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
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15% of the activity with L-valine, A124G mutant: 5.03%
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r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
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15% of the activity with L-valine
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?
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
-
8% of the activity with L-valine
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?
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
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-
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
-
24.5% of the activity with L-valine
reductive amination: 20% of the activity with 2-oxoisovalerate, 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
-
VDH2: 24% of the activity with L-valine
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate, VDH2: reductive amination, 20% of the activity with 2-oxoisovalerate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
-
-
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
-
-
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate
r
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
-
VDH2: 18.5% of the activity with L-valine
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-
r
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
-
4.7% of the activity with L-valine
reductive amination: 9.7% of the activity with 2-oxoisovalerate, 2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate
r
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
-
15% of the activity with L-valine, A124G mutant: 15%
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r
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
-
15% of the activity with L-valine
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-
?
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
-
VDH2: 34.5% of the activity with L-valine
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-
r
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
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51.5% of the activity with L-valine
2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate, reductive amination: 43.8% of the activity with 2-oxoisovalerate
r
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
-
-
2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
-
VDH2: 100% of the activity with L-valine
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-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
-
47.2% of the activity with L-leucine
reductive amination: 37% of the activity with 2-oxoisovalerate, 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
-
100.5% of the activity of L-valine, A124G mutant: 28.77%
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-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
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as active as with L-valine
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-
?
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
-
98% of the activity with L-valine
reductive amination: 79.5% of the activity with 2-oxoisovalerate, 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
-
VDH2: 96% of the activity with L-valine
-
-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
-
-
optimal chain length for reductive amination, 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate
r
L-phenylalanine + H2O + NAD+
2-oxo-3-phenylpropanoic acid + NH3 + NADH
-
only 0.95% of the activity with L-valine
-
-
r
L-phenylalanine + H2O + NAD+
2-oxo-3-phenylpropanoic acid + NH3 + NADH
-
1.25% of the activity with L-valine, A124G mutant: 2.5%
-
-
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 2.5:1
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 5:1
-
-
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
-
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
first step in valine catabolism
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
-
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
first step in valine catabolism
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
first step in valine catabolism
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 2.5:1
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 5:1
-
-
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
NADPH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
NADPH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
soluble enzyme in roots: ratio of rate of reductive amination of valine ketoanalogue to the rate of oxidative deamination of L-valine is 1:1
-
-
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
soluble enzyme in roots: ratio of rate of reductive amination of valine ketoanalogue to the rate of oxidative deamination of L-valine is 1:1
NADPH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
soluble enzyme in roots: ratio of rate of reductive amination of valine ketoanalogue to the rate of oxidative deamination of L-valine is 1:1
NADPH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
soluble enzyme in roots: ratio of rate of reductive amination of valine ketoanalogue to the rate of oxidative deamination of L-valine is 1:1
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
soluble enzyme in roots: ratio of rate of reductive amination of valine ketoanalogue to the rate of oxidative deamination of L-valine is 1:1
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
rate of reductive amination of ketoanalogue of valine 5 times greater than rate of oxidative deamination of L-valine
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 2.5:1
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 5:1
-
-
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
two forms of enzyme activity: enzyme associated with subcellular structures in shoots or leaves probably plays a biosynthetic role for valine production and the soluble enzyme in roots plays a degradative role in valine catabolism
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
Plantago sp.
-
NADP+
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
Plantago sp.
-
in the enzyme associated with subcellular structures in shoots it is 2.5:1
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
Plantago sp.
-
in the enzyme associated with subcellular structures in shoots it is 5:1
-
-
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
88.9% of the activity with L-leucine
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
preferred substrate
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
A124G mutant: 3.75% of the activity with L-valine in wild type
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
first catabolic step of L-valine and some other branched chain L-amino acids, important role in providing precursors for biosynthesis of polyether antibiotic, salinomycin, that is produced at a high level
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
L-valine oxidized efficiently
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
first step in valine catabolism
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
L-valine oxidized efficiently
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
preferred substrate
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
preferred substrate
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
preferred substrate
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
preferred substrate
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
preferred substrate
reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
preferred substrate
reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
reductive amination rate is twice the oxidative deamination rate
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
VDH2 rather than VDH1 plays a role in metabolism of branched chain amino acids and thus in tylosin biosynthesis, expression of VDH1 is unstable
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
regulatory enzyme involved in biosynthesis of n-butyrate, a building unit of the oligoketide antibiotic tylosin, NAD+ as natural cofactor for oxidative deamination
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
first step in valine catabolism
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
-
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
first step in valine catabolism
-
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
preferred substrate
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
reductive amination: preferred substrate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
first step in valine catabolism
-
?
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 2.5:1
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 5:1
-
-
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NADP+
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 2.5:1
NADPH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
in the enzyme associated with subcellular structures in shoots it is 5:1
-
-
r
additional information
additional information
-
-
no oxidative deamination of L-isoleucine and L-alanine
no reductive amination of 2-oxobutyrate
?
additional information
additional information
-
-
no oxidative deamination of L-isoleucine and L-alanine
no reductive amination of 2-oxobutyrate
?
additional information
additional information
-
-
no activity with the D-isomers of the substrates
-
-
?
additional information
additional information
-
-
Lys-79, Lys-91 are involved in substrate binding and catalysis
-
-
?
additional information
additional information
-
-
Lys-79, Lys-91 are involved in substrate binding and catalysis
-
-
?
additional information
additional information
-
-
Ala-124 is involved in substrate binding, but less of nicotinamide coenzyme
-
-
?
additional information
additional information
-
-
not: D-valine
-
-
?
additional information
additional information
-
-
not: D-valine
-
-
?