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Literature summary for 1.4.1.23 extracted from

  • Ohshima, T.; Soda, K.
    Valine dehydrogenase from a non-spore-forming bacterium, Alcaligenes faecalis: purification and characterization (1993), Biochim. Biophys. Acta, 1162, 221-226.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4,4'-Dithiopyridine 0.01 mM, 95% inhibition Alcaligenes faecalis
5,5'-dithiobis(2-nitrobenzoic acid) 0.01 mM, 95% inhibition Alcaligenes faecalis
CuSO4 1 mM, 13% inhibition Alcaligenes faecalis
HgCl2 0.1 mM, 91% inhibition Alcaligenes faecalis
additional information not inhibitory at 1 mM: ATP, ADP, AMP, GTP, GDP, MgSO4, MgCl2, ZnCl2, COSO4, BaCl2, EDTA and o-phenanthroline Alcaligenes faecalis
N-ethylmaleimide 0.01 mM, 73% inhibition Alcaligenes faecalis
p-chloromercuribenzoate 0.001 mM, 95% inhibition Alcaligenes faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Alcaligenes faecalis
72000
-
gel filtration Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH + H+ 22% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-2-aminobutanoate + H2O + NAD+
-
r
2-oxohexanoate + NH3 + NADH + H+ 6.3% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-norleucine + H2O + NAD+
-
r
2-oxoisohexanoate + NH3 + NADH + H+ 58% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-leucine + H2O + NAD+
-
r
2-oxoisovalerate + NH3 + NADH + H+
-
Alcaligenes faecalis L-valine + H2O + NAD+
-
r
2-oxovalerate + H2O + NAD+ 5.0% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-norvaline + NH3 + NADH + H+
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+ 68% of the activity with 2-oxoisovalerate Alcaligenes faecalis L-isoleucine + H2O + NAD+
-
r
L-2-aminobutanoate + H2O + NAD+ 33% of the activity with L-valine Alcaligenes faecalis 2-oxobutanoate + NH3 + NADH + H+
-
r
L-isoleucine + H2O + NAD+ 72% of the activity with L-valine Alcaligenes faecalis 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
L-leucine + H2O + NAD+ 73% of the activity with L-valine Alcaligenes faecalis 2-oxoisohexanoate + NH3 + NADH + H+
-
r
L-norleucine + H2O + NAD+ 16% of the activity with L-valine Alcaligenes faecalis 2-oxohexanoate + NH3 + NADH + H+
-
r
L-norvaline + H2O + NAD+ 44% of the activity with L-valine Alcaligenes faecalis 2-oxovalerate + NH3 + NADH + H+
-
r
L-valine + H2O + NAD+
-
Alcaligenes faecalis 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Alcaligenes faecalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Alcaligenes faecalis
45
-
5 min, 50% loss of activity Alcaligenes faecalis
50
-
5 min, complete loss of activity Alcaligenes faecalis
55
-
5 min, presence of 20% glycerol, retains full activity Alcaligenes faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
reductive amination Alcaligenes faecalis
10.7
-
oxidative demaination Alcaligenes faecalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9.7 stable for at least 5 min Alcaligenes faecalis

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine-deamino-NAD+ 150% of the activity with NAD+ Alcaligenes faecalis
3-acetylpyridine-NAD+ 714% of the activity with NAD+ Alcaligenes faecalis
3-pyridinealdehyde-NAD+ 173% of the activity with NAD+ Alcaligenes faecalis
deamino-NAD+ 440% of the activity with NAD+ Alcaligenes faecalis
additional information no cofactor: NADP+ Alcaligenes faecalis
NAD+
-
Alcaligenes faecalis
NADH
-
Alcaligenes faecalis