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Information on EC 1.4.1.23 - valine dehydrogenase (NAD+)
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1.4.1.23 valine dehydrogenase (NAD+)IUBMB Comments
The enzyme from Streptomyces spp. has no activity with NADP+ [cf. EC 1.4.1.8, valine dehydrogenase (NADP+)].
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The enzyme appears in viruses and cellular organisms
Synonyms
VDH, VDH2, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
VDH
VDH2
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+
sequential ordered mechanism with non-competitive inhibition by valine
-
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+
the reductive amination proceeds through a sequential ordered ternary-binary mechanism. NADH binds to the enzyme first, followed by 2-oxoisovalerate and NH3, and valine is released first, followed by NAD+
-
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+
sequential ordered mechanism with non-competitive inhibition by valine
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
L-valine:NAD+ oxidoreductase (deaminating)
The enzyme from Streptomyces spp. has no activity with NADP+ [cf. EC 1.4.1.8, valine dehydrogenase (NADP+)].
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
-
very poor substrate: 1.2% of the activity with L-valine
pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate, reductive amination: very poor substrate, 1.3% of the activity with 2-oxoisovalerate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
-
46.5% of the activity with L-valine
2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate, reductive amination: 22.3% of the activity with 2-oxoisovalerate
r
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
-
36% of the activity with L-valine
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate, reductive amination: 38.1% of the activity with 2-oxoisovalerate
r
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
-
10.5% of the activity with L-valine
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
22% of the activity with 2-oxoisovalerate
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
116% of the activity with 2-oxoisovalerate
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
48.2% of the activity with 2-oxoisovalerate
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
100% of the activity with 2-oxoisovalerate
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
78% of the activity with 2-oxoisovalerate
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
-
r
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
-
6.3% of the activity with 2-oxoisovalerate
-
-
r
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
-
43.8% of the activity with 2-oxoisovalerate
-
-
r
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
58% of the activity with 2-oxoisovalerate
-
-
r
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
26.5% of the activity with 2-oxoisovalerate
-
-
r
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
38.1% of the activity with 2-oxoisovalerate
-
-
r
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
20% of the activity with 2-oxoisovalerate
-
-
r
2-oxoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
-
-
-
-
r
2-oxoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
-
-
-
-
r
2-oxoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
-
-
the 4'-pro-S hydrogen atom of NADH is transferred to the substrate in the reductive-amination reaction
-
r
2-oxovalerate + H2O + NAD+
L-norvaline + NH3 + NADH + H+
-
5.0% of the activity with 2-oxoisovalerate
-
-
r
2-oxovalerate + H2O + NAD+
L-norvaline + NH3 + NADH + H+
-
79.5% of the activity with 2-oxoisovalerate
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
68% of the activity with 2-oxoisovalerate
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
22.3% of the activity with 2-oxoisovalerate
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
24.5% of the activity with 2-oxoisovalerate
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
16.6% of the activity with 2-oxoisovalerate
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
23.2% of the activity with 2-oxoisovalerate
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
-
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
33% of the activity with L-valine
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
16.5% of the activity with L-valine
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
125% of the activity with L-valine
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
16.5% of the activity with L-valine
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
130% of the activity with L-valine
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
68.9% of the activity with L-valine
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
158% of the activity with L-valine
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
16.5% of the activity with L-valine
reductive amination: 48.2% of the activity with 2-oxoisovalerate
r
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
-
L-2-aminobutyrate is identical with L-alpha-aminobutyrate
reductive amination: 48.2% of the activity with 2-oxoisovalerate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
1.9% of the activity with L-valine
-
-
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
4.5% of the activity with L-valine
-
-
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
3% of the activity with L-valine
-
-
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
14.7% of the activity with L-valine
-
-
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
-
8.3% of the activity with L-valine
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
72% of the activity with L-valine
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
46.5% of the activity with L-valine
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
34% of the activity with L-valine
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
28% of the activity with L-valine
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
29.4% of the activity with L-valine
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
22% of the activity with L-valine
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
73% of the activity with L-valine
-
-
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
36% of the activity with L-valine
-
-
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
52.8% of the activity with L-valine
-
-
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
8% of the activity with L-valine
-
-
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
24.5% of the activity with L-valine
-
-
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
-
24% of the activity with L-valine
-
-
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
16% of the activity with L-valine
-
-
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
10.5% of the activity with L-valine
-
-
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
18.5% of the activity with L-valine
-
-
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
51.5% of the activity with L-valine
-
-
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
-
34.5% of the activity with L-valine
-
-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
-
43% of the activity with L-valine
-
-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
-
43% of the activity with L-valine
-
-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
44% of the activity with L-valine
-
-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
100% of the activity with L-valine
-
-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
43.0% of the activity with L-valine
-
-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
98% of the activity with L-valine
-
-
r
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
-
96% of the activity with L-valine
-
-
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, highest activity with 2-oxoisovalerate
r
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
-
NAD+
NADH, 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate, highest activity with 2-oxoisovalerate
r
pyruvate + H2O + NAD+
L-alanine + NH3 + NADH + H+
-
1.3% of the activity with 2-oxoisovalerate
-
-
r
pyruvate + H2O + NAD+
L-alanine + NH3 + NADH + H+
-
20.8% of the activity with 2-oxoisovalerate
-
-
r
pyruvate + H2O + NAD+
L-alanine + NH3 + NADH + H+
-
23.3% of the activity with 2-oxoisovalerate
-
-
r
pyruvate + NH3 + NADH + H+
L-alanine + H2O + NAD+
-
31.6% of the activity with 2-oxoisovalerate
-
-
r
additional information
?
-
-
no substrates: D-valine, glycine, L-threonine, L-serine, L-cysteine, L-glutamate, L-aspartate, L-asparagine, L-arginine, L-histidine, L-tryptophan, and DL-phenylalanine, and 2-ketoglutarate and oxalacetate
-
-
?
additional information
?
-
-
no substrates: D-valine, D-leucine, D-isoleucine, glycine, L-threonine, fialanine, L-serine, L-cysteine, L-methionine, L-glutamic acid, L-aspartic acid, L-asparagine, L-glutamine, L-lysine, L-phenylalanine, L-tyrosine, L-histidine and L-tryptophan as well as 2-oxoglutarate, oxalacetate, phenylpyruvate and glyoxalate
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
nicotinamide guanine dinucleotide
-
72.3% of the activity with NAD+
additional information
-
no cofactor: 3-acetylpyridine-NAD+, thionicotinamide-NAD+, alpha-NAD+, 3-pyridinaldehyde-NAD+, deamido-NAD+
-
additional information
-
no cofactors: deamido-NAD+, 3-pyridinealdehyde-NAD+, thionicotinamide-NAD+
-
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
not inhibitory at 1 mM: ATP, ADP, AMP, GTP, GDP, MgSO4, MgCl2, ZnCl2, COSO4, BaCl2, EDTA and o-phenanthroline
-
additional information
-
no inhibition by nucleotide mono-, di-, triphosphates or the free bases adenine, cytosine, thymine, guanine and uracil
-
additional information
-
activity is not significantly affected by 1 mM ATP, ADP, AMP, Mg2+, Ca2+, Cu2+, Zn2+, and metabolites of the citric acid cycle
-
additional information
-
not inhibitory: EDTA, benzamidine, PMSF, all at 5 mM, or citrate, cobalt(II), azide, Li+ and Mn2+, at 1 mM
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.2
2-oxobutanoate
-
pH 10.4, temperature not specified in the publication
0.42
2-Oxoisohexanoate
-
pH 10.4, temperature not specified in the publication
0.46
2-oxoisovalerate
-
pH 10.4, temperature not specified in the publication
1.06
3-methyl-2-oxopentanoate
-
pH 10.4, temperature not specified in the publication
4.35
L-2-aminobutanoate
-
pH 10.4, temperature not specified in the publication
333
L-alanine
-
pH 10.4, temperature not specified in the publication
1.72
L-isoleucine
-
pH 10.4, temperature not specified in the publication
1.85
L-leucine
-
pH 10.4, temperature not specified in the publication
22.5
L-norleucine
-
pH 10.4, temperature not specified in the publication
1.66
L-norvaline
-
pH 10.4, temperature not specified in the publication
0.75
L-valine
-
pH 10.4, temperature not specified in the publication
17.2
pyruvate
-
pH 10.4, temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE