Information on EC 1.4.1.23 - valine dehydrogenase (NAD+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.4.1.23
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RECOMMENDED NAME
GeneOntology No.
valine dehydrogenase (NAD+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Valine, leucine and isoleucine degradation
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Metabolic pathways
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Biosynthesis of secondary metabolites
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
L-valine:NAD+ oxidoreductase (deaminating)
The enzyme from Streptomyces spp. has no activity with NADP+ [cf. EC 1.4.1.8, valine dehydrogenase (NADP+)].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
a UV mutant derived from the high-producing strain 84/25
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
show the reaction diagram
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
show the reaction diagram
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
show the reaction diagram
2-oxoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
show the reaction diagram
2-oxoisovalerate + NH4+ + NADH
L-valine + H2O + NAD+
show the reaction diagram
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-
-
-
r
2-oxovalerate + H2O + NAD+
L-norvaline + NH3 + NADH + H+
show the reaction diagram
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
show the reaction diagram
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
show the reaction diagram
L-2-aminobutyrate + H2O + NAD+
2-oxobutyrate + NADH + NH3
show the reaction diagram
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH
show the reaction diagram
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very poor substrate: 1.2% of the activity with L-valine
pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate, reductive amination: very poor substrate, 1.3% of the activity with 2-oxoisovalerate
r
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
show the reaction diagram
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NADH + NH3
show the reaction diagram
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46.5% of the activity with L-valine
2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate, reductive amination: 22.3% of the activity with 2-oxoisovalerate
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
show the reaction diagram
L-leucine + H2O + NAD+
2-oxoisocaproate + NADH + NH3
show the reaction diagram
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36% of the activity with L-valine
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate, reductive amination: 38.1% of the activity with 2-oxoisovalerate
r
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
show the reaction diagram
L-norleucine + H2O + NAD+
2-oxocaproate + NADH + NH3
show the reaction diagram
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10.5% of the activity with L-valine
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-
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
show the reaction diagram
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH
show the reaction diagram
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
show the reaction diagram
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
show the reaction diagram
L-valine + NAD(P)+ + H2O
2-oxoisovalerate + NH3 + NAD(P)H
show the reaction diagram
pyruvate + H2O + NAD+
L-alanine + NH3 + NADH + H+
show the reaction diagram
pyruvate + NH3 + NADH + H+
L-alanine + H2O + NAD+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,N6-etheno-NAD+
3-acetylpyridine-deamino-NAD+
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150% of the activity with NAD+
3-acetylpyridine-NAD+
3-pyridinealdehyde-NAD+
Alpha-NAD+
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3.6% of the activity with NAD+
deamino-NAD+
NADP+
nicotinamide guanine dinucleotide
additional information
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4,4'-Dithiopyridine
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0.01 mM, 95% inhibition
4-hydroxymercuribenzoate
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0.01 mM, 100% inhibition
5,5'-dithiobis(2-nitrobenzoic acid)
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0.01 mM, 95% inhibition
Ag+
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0.1 mM, strong inhibition
Ca2+
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1 mM, partial inhibition
Cu2+
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0.1 mM, strong inhibition
CuSO4
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1 mM, 13% inhibition
Hg+
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0.1 mM, strong inhibition
HgCl2
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0.1 mM, 91% inhibition
iodoacetamide
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1 mM, 50% inhibition
L-valine
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substrate inhibition, noncompetitive
Mg2+
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1 mM, partial inhibition
Mn2+
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1 mM, 60% inhibition
N-ethylmaleimide
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0.01 mM, 73% inhibition
p-chloromercuribenzoate
Zn2+
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1 mM, partial inhibition
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.7
2-oxo-3-methylvalerate
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0.13 - 1.5
2-oxobutanoate
1.5
2-oxobutyrate
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0.81 - 2.23
2-Oxohexanoate
0.8
2-oxoisocaproate
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-
0.42 - 0.82
2-Oxoisohexanoate
0.31 - 1.3
2-oxoisovalerate
0.48 - 1.94
2-oxovalerate
1.06 - 6.7
3-methyl-2-oxopentanoate
3.07 - 19.6
L-2-aminobutanoate
14.8
L-2-aminobutyrate
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28.2 - 333.6
L-alanine
1.18 - 10.8
L-isoleucine
0.67 - 6.3
L-leucine
6.68 - 30
L-norleucine
1.33 - 5.7
L-norvaline
0.43 - 2.5
L-valine
0.023 - 0.1
NAD+
0.023 - 0.028
NADH
18.2
NH3
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pH 10.4, 30C
18.2 - 25.6
NH4+
12.3 - 25
pyruvate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
99
2-oxobutanoate
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pH 9.5, 21C
107
2-Oxohexanoate
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pH 9.5, 21C
71.9
2-Oxoisohexanoate
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pH 9.5, 21C
226
2-oxoisovalerate
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pH 9.5, 21C
164
2-oxovalerate
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pH 9.5, 21C
104
L-2-aminobutanoate
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pH 10.0, 21C
5.8
L-isoleucine
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pH 10.0, 21C
11.9
L-leucine
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pH 10.0, 21C
17.9
L-norleucine
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pH 10.0, 21C
18
L-norvaline
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pH 10.0, 21C
28.3
L-valine
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pH 10.0, 21C
114
pyruvate
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pH 9.5, 21C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
740
2-oxobutanoate
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pH 9.5, 21C
131
2-Oxohexanoate
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pH 9.5, 21C
143
2-Oxoisohexanoate
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pH 9.5, 21C
279
2-oxoisovalerate
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pH 9.5, 21C
340
2-oxovalerate
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pH 9.5, 21C
5.3
L-2-aminobutanoate
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pH 10.0, 21C
0.53
L-isoleucine
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pH 10.0, 21C
3
L-leucine
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pH 10.0, 21C
0.6
L-norleucine
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pH 10.0, 21C
5.6
L-norvaline
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pH 10.0, 21C
21.8
L-valine
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pH 10.0, 21C
9.3
pyruvate
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pH 9.5, 21C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14
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pH 10.4, 30C, substrate L-valine
19
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pH 10.4, 30C, substrate 2-aminobutanoate
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.8
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reductive amination
9.5
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reductive amination
10
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oxidative deamination
10.5
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oxidative deamination
10.6
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oxidative deamination
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7
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isoelectric focusing
5.1
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isoelectric focusing
6.2
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isoelectric focusing
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
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12 * 18000, SDS-PAGE
29000
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4 * 29000, SDS-PAGE
40000
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2 * 40000, SDS-PAGE
70000
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gel filtration
72000
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gel filtration
80000
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gel filtration
88000
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gel filtration
116000 - 118000
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gel filtration and equilibrium ultracentrifugation
116000
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equilibrium ultracentrifugation
118000
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gel filtration
215000 - 218000
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gel filtration and equilibrium ultracentrifugation
240000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dodecamer
hexamer
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.7
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stable for at least 5 min
717267
7
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dramatic decrease in stability below
391694
7.4
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most stable at
391694
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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stable up to
45
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5 min, 50% loss of activity
50
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5 min, complete loss of activity
55
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5 min, presence of 20% glycerol, retains full activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, pH 7.4, presence of 2-mercaptoethanol and 30% glycerol, loss of 90% of activity with 24 h
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-25C, 0.2 M Tris-HCl buffer, pH 7.4, 2 months, no loss of activity
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-80C, presence of 20% glycerol, stable for at least 2 months
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4C, 70% (NH4)2SO4 suspension, 3 weeks, no loss of activity
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4C, pH 7.4, presence of 2-mercaptoethanol and 10% glycerol, stable for 48 h
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
activity is induced in presence of D- and L-valine
activity is induced in presence of L-valine
activity is repressed in presence of ammonia
activity is repressed in the presence of glucose and NH4+ or glycerol and NH4+ or asparagine