Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.23 extracted from

  • Nguyen, L.T.; Nguyen, K.T.; Spizek, J.; Behal, V.
    The tylosin producer, Streptomyces fradiae, contains a second valine dehydrogenase (1995), Microbiology, 141, 1139-1145.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
NADH pH 10.4, 30°C Streptomyces fradiae
0.04
-
NAD+ pH 10.4, 30°C Streptomyces fradiae
0.31
-
2-oxoisovalerate pH 10.4, 30°C Streptomyces fradiae
0.43
-
L-valine pH 10.4, 30°C Streptomyces fradiae
25.6
-
NH4+ pH 10.4, 30°C Streptomyces fradiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
2 * 41000, SDS-PAGE Streptomyces fradiae
80000
-
gel filtration Streptomyces fradiae

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces fradiae

Storage Stability

Storage Stability Organism
-20°C, pH 7.4, presence of 2-mercaptoethanol and 30% glycerol, loss of 90% of activity with 24 h Streptomyces fradiae
4°C, pH 7.4, presence of 2-mercaptoethanol and 10% glycerol, stable for 48 h Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH + H+ 100% of the activity with 2-oxoisovalerate Streptomyces fradiae L-2-aminobutanoate + H2O + NAD+
-
r
2-oxoisohexanoate + NH3 + NADH + H+ 20% of the activity with 2-oxoisovalerate Streptomyces fradiae L-leucine + H2O + NAD+
-
r
2-oxoisovalerate + NH3 + NADH + H+
-
Streptomyces fradiae L-valine + H2O + NAD+
-
r
2-oxoisovalerate + NH4+ + NADH
-
Streptomyces fradiae L-valine + H2O + NAD+
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+ 16.6% of the activity with 2-oxoisovalerate Streptomyces fradiae L-isoleucine + H2O + NAD+
-
r
L-2-aminobutanoate + H2O + NAD+ 158% of the activity with L-valine Streptomyces fradiae 2-oxobutanoate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+ 8.3% of the activity with L-valine Streptomyces fradiae pyruvate + NH3 + NADH + H+
-
r
L-isoleucine + H2O + NAD+ 22% of the activity with L-valine Streptomyces fradiae 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
L-leucine + H2O + NAD+ 24% of the activity with L-valine Streptomyces fradiae 2-oxoisohexanoate + NH3 + NADH + H+
-
r
L-norleucine + H2O + NAD+ 34.5% of the activity with L-valine Streptomyces fradiae 2-oxohexanoate + NH3 + NADH + H+
-
r
L-norvaline + H2O + NAD+ 96% of the activity with L-valine Streptomyces fradiae 2-oxovalerate + NH3 + NADH + H+
-
r
L-valine + H2O + NAD+
-
Streptomyces fradiae 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r
pyruvate + H2O + NAD+ 23.3% of the activity with 2-oxoisovalerate Streptomyces fradiae L-alanine + NH3 + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Streptomyces fradiae

Synonyms

Synonyms Comment Organism
VDH2
-
Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
both oxidative deamination and reductive amination Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
reductive amination Streptomyces fradiae
10.4
-
oxidative deamination Streptomyces fradiae

Cofactor

Cofactor Comment Organism Structure
1,N6-etheno-NAD+ 70.5% of the activity with NAD+ Streptomyces fradiae
3-pyridinealdehyde-NAD+ 47% of the activity with NAD+ Streptomyces fradiae
deamino-NAD+ 124% of the activity with NAD+ Streptomyces fradiae
additional information no activity with NADP+ Streptomyces fradiae
NAD+
-
Streptomyces fradiae
NADH
-
Streptomyces fradiae

Expression

Organism Comment Expression
Streptomyces fradiae activity is repressed in presence of ammonia down
Streptomyces fradiae activity is induced in presence of L-valine up