Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.99.31 extracted from

  • Seo, Y.; Choi, S.; Nam, S.; Kim, G.
    Molecular cloning and characterization of the gene encoding phytoene desaturase from Kocuria gwangalliensis (2017), Microbiol. Biotechnol. Lett., 45, 226-235 .
No PubMed abstract available
Search for more literature for 1.3.99.31

Cloned(Commentary)

Cloned (Comment) Organism
gene crtI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant enzyme expression from plasmid pCcrt1 in Escherichia coli. Lycopene biosynthesis is confirmed when the plasmid pCcrtI is cotransformed into Escherichia coli containing the plasmid pRScrtEB carrying the crtE and crtB genes required for lycopene biosynthesis Kocuria gwangalliensis

Protein Variants

Protein Variants Comment Organism
additional information lycopene biosynthesis is confirmed when the plasmid pCcrtI is cotransformed into Escherichia coli containing the plasmid pRScrtEB carrying the crtE and crtB genes required for lycopene biosynthesis Kocuria gwangalliensis

Organism

Organism UniProt Comment Textmining
Kocuria gwangalliensis H2DDY5
-
-

Subunits

Subunits Comment Organism
? x * 57000, recombinant CrtI, SDS-PAGE Kocuria gwangalliensis

Synonyms

Synonyms Comment Organism
CrtI
-
 Kocuria gwangalliensis
phytoene desaturase
-
 Kocuria gwangalliensis

General Information

General Information Comment Organism
evolution the nucleotide sequence of the crtI gene compared with that of other species, including Kocuria rhizophila and Myxococcus xanthus, proves well conserved during evolution Kocuria gwangalliensis
metabolism the enzyme is involved in the lycopene biosynthetic pathway, overview Kocuria gwangalliensis
physiological function phytoene desaturase (CrtI), encoded by the gene crtI, catalyzes lycopene formation from phytoene and is an essential enzyme in the early steps of carotenoid biosynthesis. CrtI is one of the key enzymes regulating carotenoid biosynthesis and has been implicated as a rate-limiting enzyme of the pathway in various carotenoid synthesizing organisms Kocuria gwangalliensis