Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pantoea ananatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pantoea ananatis | |
NADP+ | - |
Pantoea ananatis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pantoea ananatis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56200 | - |
x * 56200, SDS-PAGE | Pantoea ananatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 4 acceptor | Pantoea ananatis | overall reaction. all-trans-Lycopene is the principal desaturase product constituting 60% of the total reaction products. The desaturase intermediates all-trans-phytofluene and all-trans-zeta-carotene are also detected and constitute 27 and 12% of the total desaturase products formed | all-trans-lycopene + 4 reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | Pantoea ananatis | - |
all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-phytofluene + 3 acceptor | Pantoea ananatis | - |
all-trans-lycopene + 3 reduced acceptor | - |
? | |
all-trans-zeta-carotene + 2 acceptor | Pantoea ananatis | - |
all-trans-lycopene + 2 reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea ananatis | P21685 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pantoea ananatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 4 acceptor | overall reaction. all-trans-Lycopene is the principal desaturase product constituting 60% of the total reaction products. The desaturase intermediates all-trans-phytofluene and all-trans-zeta-carotene are also detected and constitute 27 and 12% of the total desaturase products formed | Pantoea ananatis | all-trans-lycopene + 4 reduced acceptor | - |
? | |
all-trans-neurosporene + acceptor | - |
Pantoea ananatis | all-trans-lycopene + reduced acceptor | - |
? | |
all-trans-phytofluene + 3 acceptor | - |
Pantoea ananatis | all-trans-lycopene + 3 reduced acceptor | - |
? | |
all-trans-zeta-carotene + 2 acceptor | - |
Pantoea ananatis | all-trans-lycopene + 2 reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56200, SDS-PAGE | Pantoea ananatis |
Synonyms | Comment | Organism |
---|---|---|
CrtI | - |
Pantoea ananatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pantoea ananatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | involved in desaturation. FAD is a dissociable moiety and not covalently bound to the enzyme | Pantoea ananatis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in carotenoid biosynthesis | Pantoea ananatis |