BRENDA - Enzyme Database show
show all sequences of 1.3.7.4

Holophytochrome assembly. Coupled assay for phytochromobilin synthase in organello

Terry, M.J.; Lagarias, J.C.; J. Biol. Chem. 266, 22215-22221 (1991)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ATP
stimulates at 1 mM
Cucumis sativus
additional information
NADPH regenerating system with glucose-6-phosphate and glucose-6-phosphate dehydrogenase stimulates
Cucumis sativus
NADH
stimulates slightly at 1 mM
Cucumis sativus
NADPH
stimulates slightly at 1 mM
Cucumis sativus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
etiolated
Cucumis sativus
9536
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3Z)-phytochromobilin + oxidized ferredoxin
Cucumis sativus
-
biliverdin IXa + reduced ferredoxin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cucumis sativus
-
cucumber
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Cucumis sativus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000004
-
in etiolated plastids
Cucumis sativus
0.0000011
-
specific activity of plastid protein
Cucumis sativus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3Z)-phytochromobilin + oxidized ferredoxin
-
437769
Cucumis sativus
biliverdin IXa + reduced ferredoxin
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ATP
stimulates at 1 mM
Cucumis sativus
additional information
NADPH regenerating system with glucose-6-phosphate and glucose-6-phosphate dehydrogenase stimulates
Cucumis sativus
NADH
stimulates slightly at 1 mM
Cucumis sativus
NADPH
stimulates slightly at 1 mM
Cucumis sativus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
etiolated
Cucumis sativus
9536
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3Z)-phytochromobilin + oxidized ferredoxin
Cucumis sativus
-
biliverdin IXa + reduced ferredoxin
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Cucumis sativus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0000004
-
in etiolated plastids
Cucumis sativus
0.0000011
-
specific activity of plastid protein
Cucumis sativus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3Z)-phytochromobilin + oxidized ferredoxin
-
437769
Cucumis sativus
biliverdin IXa + reduced ferredoxin
-
-
-
?
Other publictions for EC 1.3.7.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742513
Shin
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Arabidopsis thaliana
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2014
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1
1
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724317
Alvey
Attachment of noncognate chrom ...
Arabidopsis thaliana
Biochemistry
50
4890-4902
2011
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1
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8
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1
1
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725252
Alvey
Effects of modified phycobilin ...
Arabidopsis thaliana
J. Bacteriol.
193
1663-1671
2011
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1
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1
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712484
Chiu
Electrostatic interaction of p ...
Arabidopsis thaliana
J. Biol. Chem.
285
5056-5065
2010
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9
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1
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1
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4
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2
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1
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9
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1
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1
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2
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1
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1
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1
1
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687760
Tu
Mechanistic studies of the phy ...
Arabidopsis thaliana
J. Biol. Chem.
283
27555-27564
2008
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1
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5
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2
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1
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1
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1
1
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5
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1
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685125
Tu
Insight into the radical mecha ...
Arabidopsis thaliana
Biochemistry
46
1484-1494
2007
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1
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1
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673636
Mukougawa
Metabolic engineering to produ ...
Escherichia coli
FEBS Lett.
580
1333-1338
2006
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1
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1
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1
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743476
Creff
Targeted Ds-tagging strategy ...
Arabidopsis thaliana
Plant Mol. Biol.
61
603-613
2006
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1
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1
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1
1
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676460
Muramoto
The tomato photomorphogenetic ...
Solanum lycopersicum
Plant Cell Physiol.
46
661-665
2005
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4
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657069
Sawers
The Elm1 (ZmHy2) gene of maize ...
Zea mays
Plant Physiol.
136
2771-2781
2004
-
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1
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1
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1
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5
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1
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437673
Frankenberg
Functional genomic analysis of ...
Arabidopsis thaliana
Plant Cell
13
965-978
2001
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1
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1
1
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6
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1
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2
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2
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437767
Kohchi
The Arabidopsis hy2 gene encod ...
Arabidopsis thaliana
Plant Cell
13
425-436
2001
-
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1
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1
-
2
-
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4
-
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1
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1
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1
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1
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1
1
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1
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2
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1
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1
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1
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437768
McDowell
Purification and biochemical p ...
Avena sativa
Plant Physiol.
126
1546-1554
2001
-
-
-
-
-
-
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3
1
-
1
-
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4
-
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1
-
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2
-
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1
1
1
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1
1
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1
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1
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3
1
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1
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1
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2
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1
1
1
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1
1
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437769
Terry
Holophytochrome assembly. Coup ...
Cucumis sativus
J. Biol. Chem.
266
22215-22221
1991
4
-
-
-
-
-
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1
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1
-
3
-
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1
2
-
1
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-
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4
-
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1
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1
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1
2
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1
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