BRENDA - Enzyme Database
show all sequences of 1.3.1.116

Clostridium scindens baiCD and baiH genes encode stereo-specific 7alpha/7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductases

Kang, D.; Ridlon, J.; Moore II, D.; Barnes, S.; Hylemon, P.; Biochim. Biophys. Acta 1781, 16-25 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Clostridium scindens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72000
-
SDS-PAGE
Clostridium scindens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens
-
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens VPI 12708
-
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens VPI 12708
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens JCM 10418
-
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens JCM 10418
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Clostridium scindens
P32370
-
-
Clostridium scindens JCM 10418
P32370
-
-
Clostridium scindens VPI 12708
P32370
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Clostridium scindens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
16.4
-
37°C, pH 6.8; pH 6.8, 25°C
Clostridium scindens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
744383
Clostridium scindens
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
744383
Clostridium scindens
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer
744383
Clostridium scindens
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
744383
Clostridium scindens VPI 12708
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
744383
Clostridium scindens VPI 12708
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer
744383
Clostridium scindens VPI 12708
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
744383
Clostridium scindens JCM 10418
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
744383
Clostridium scindens JCM 10418
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer
744383
Clostridium scindens JCM 10418
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 72000, SDS-PAGE
Clostridium scindens
homotrimer
3 * 72000, SDS-PAGE
Clostridium scindens
Synonyms
Synonyms
Commentary
Organism
7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductase
-
Clostridium scindens
BaiH
-
Clostridium scindens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Clostridium scindens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Clostridium scindens
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit
Clostridium scindens
FMN
flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit
Clostridium scindens
additional information
no cofactor: NADP+
Clostridium scindens
NAD+
-
Clostridium scindens
NADH
-
Clostridium scindens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Clostridium scindens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit
Clostridium scindens
FMN
flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit
Clostridium scindens
additional information
no cofactor: NADP+
Clostridium scindens
NAD+
-
Clostridium scindens
NADH
-
Clostridium scindens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72000
-
SDS-PAGE
Clostridium scindens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens
-
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens VPI 12708
-
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens VPI 12708
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens JCM 10418
-
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
Clostridium scindens JCM 10418
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium scindens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
16.4
-
37°C, pH 6.8; pH 6.8, 25°C
Clostridium scindens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
744383
Clostridium scindens
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
744383
Clostridium scindens
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer
744383
Clostridium scindens
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
744383
Clostridium scindens VPI 12708
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
744383
Clostridium scindens VPI 12708
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer
744383
Clostridium scindens VPI 12708
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
744383
Clostridium scindens JCM 10418
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
744383
Clostridium scindens JCM 10418
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer
744383
Clostridium scindens JCM 10418
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 72000, SDS-PAGE
Clostridium scindens
homotrimer
3 * 72000, SDS-PAGE
Clostridium scindens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Clostridium scindens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Clostridium scindens
General Information
General Information
Commentary
Organism
metabolism
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
Clostridium scindens
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme participates in the bile acid 7beta-dehydroxylation pathway
Clostridium scindens
Other publictions for EC 1.3.1.116
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744383
Kang
Clostridium scindens baiCD an ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
Biochim. Biophys. Acta
1781
16-25
2008
-
-
1
-
-
-
-
-
-
-
1
6
-
12
-
-
1
-
-
-
1
-
9
2
3
1
-
-
-
1
-
-
5
-
-
-
-
-
1
5
-
-
-
-
-
-
-
-
-
1
6
-
-
-
1
-
-
1
-
9
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
744377
Baron
Expression of the bile acid-i ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
Biochim. Biophys. Acta
1249
145-154
1995
-
-
1
-
-
-
12
-
-
2
2
-
-
7
-
-
1
-
-
-
-
-
3
2
2
1
-
-
-
1
-
-
2
-
1
-
-
-
1
2
-
-
-
-
12
-
-
-
2
2
-
-
-
-
1
-
-
-
-
3
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
745196
Franklund
Characterization of the baiH ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
J. Bacteriol.
175
3002-3012
1993
-
-
1
-
-
-
14
-
-
-
2
-
-
7
-
-
1
-
-
-
1
-
3
2
1
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
14
-
-
-
-
2
-
-
-
-
1
-
-
1
-
3
2
1
-
-
-
1
-
-
-
1
-
-
1
-
-