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Literature summary for 1.3.1.109 extracted from

  • Demmer, J.K.; Pal Chowdhury, N.; Selmer, T.; Ermler, U.; Buckel, W.
    The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile (2017), Nat. Commun., 8, 1577 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.109

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridioides difficile

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M calcium acetate, 14 % (w/v) PEG 400, 0.1 M MES, pH 6.5 Clostridioides difficile

Protein Variants

Protein Variants Comment Organism
D345A the mutant shows reduced activity compared to the wild type enzyme Clostridioides difficile
D345N the mutant shows reduced activity compared to the wild type enzyme Clostridioides difficile
E198Q inactive Clostridioides difficile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster Clostridioides difficile
-
 (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster Clostridioides difficile DSM 1296
-
 (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridioides difficile A0A125V4E7 cf. 1.3.8.1
-
Clostridioides difficile DSM 1296 A0A125V4E7 cf. 1.3.8.1
-

Purification (Commentary)

Purification (Comment) Organism
Strep tag II column chromatography and Superdex 200 gel filtration Clostridioides difficile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster
-
 Clostridioides difficile (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster
-
 Clostridioides difficile DSM 1296 (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Synonyms

Synonyms Comment Organism
electron transferring flavoprotein/butyryl-CoA dehydrogenase
-
 Clostridioides difficile
EtfAB/Bcd
-
 Clostridioides difficile

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Clostridioides difficile
Ferredoxin
-
 Clostridioides difficile
NAD+
-
 Clostridioides difficile