Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Acetoanaerobium sticklandii | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
10 * 31000, adjusted for the weight of water molecules lost as a result of peptide bond cleavage | Acetoanaerobium sticklandii |
298000 | - |
sedimentation equilibrium centrifugation | Acetoanaerobium sticklandii |
327000 | - |
gel filtration | Acetoanaerobium sticklandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-proline + NADH | Acetoanaerobium sticklandii | NADH-dependent reduction of D-proline, NADH is the normal physiological electron donor, but the purified enzyme is inactive in presence of NADH in vitro | 5-aminopentanoic acid + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetoanaerobium sticklandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.26 | - |
- |
Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-proline + dithiol (reduced) | - |
Acetoanaerobium sticklandii | 5-aminopentanoic acid + oxidized dithiol | - |
ir | |
D-proline + dithiothreitol | dithiothreitol as artificial electron donor | Acetoanaerobium sticklandii | 5-amino pentanoic acid + ? | - |
ir | |
D-proline + NADH | NADH-dependent reduction of D-proline, NADH is the normal physiological electron donor, but the purified enzyme is inactive in presence of NADH in vitro | Acetoanaerobium sticklandii | 5-aminopentanoic acid + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 30000-31000, SDS-PAGE | Acetoanaerobium sticklandii |
decamer | 10 * 31000, adjusted for the weight of water molecules lost as a result of peptide bond cleavage | Acetoanaerobium sticklandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | normal physiological electron donor in vivo, highly specific, NADPH is inactive as electron donor | Acetoanaerobium sticklandii |