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Literature summary for 1.21.1.1 extracted from

  • Phatarphekar, A.; Rokita, S.E.
    Functional analysis of iodotyrosine deiodinase from Drosophila melanogaster (2016), Protein Sci., 25, 2187-2195 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
E154Q the mutation diminishes the affinity of the enzyme for 3,5-diiodo-L-tyrosine by 30fold Drosophila melanogaster
K179Q the mutation diminishes the affinity of the enzyme for 3,5-diiodo-L-tyrosine by 46fold Drosophila melanogaster
Y158F the mutation results in a 7fold decrease in the enzyme's affinity for 3,5-diiodo-L-tyrosine Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 3-bromo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
0.014
-
 3-iodo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
0.021
-
 3-chloro-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-iodo-L-tyrosine + NADP+ + iodide Drosophila melanogaster
-
 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?
L-tyrosine + NADP+ + iodide Drosophila melanogaster
-
 3-iodo-L-tyrosine + NADPH + H+
-
 ?
L-tyrosine + NADP+ + iodide Drosophila melanogaster overall reaction 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster E1JIB2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-bromo-L-tyrosine + NADP+ + bromide
-
 Drosophila melanogaster 3,5-dibromo-L-tyrosine + NADPH + H+
-
 ?
3-chloro-L-tyrosine + NADP+ + chloride
-
 Drosophila melanogaster 3,5-dichloro-L-tyrosine + NADPH + H+
-
 ?
3-iodo-L-tyrosine + NADP+ + iodide
-
 Drosophila melanogaster 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?
L-tyrosine + NADP+ + iodide
-
 Drosophila melanogaster 3-iodo-L-tyrosine + NADPH + H+
-
 ?
L-tyrosine + NADP+ + iodide overall reaction Drosophila melanogaster 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?
additional information no activity with 3-fluoro-L-tyrosine Drosophila melanogaster ?
-
 ?

Synonyms

Synonyms Comment Organism
IYD
-
 Drosophila melanogaster

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 3-chloro-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
0.069
-
 3-bromo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
0.12
-
 3-iodo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Drosophila melanogaster
NADP+
-
 Drosophila melanogaster

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2400
-
 3-chloro-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
9000
-
 3-iodo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
9000
-
 3-bromo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster