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Literature summary for 1.21.1.1 extracted from
- Purification and characterization of a flavoprotein from bovine thyroid with iodotyrosine deiodinase activity (1979), J. Biol. Chem., 254, 12318-12325.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | maximal activity at 50 mM | Bos taurus |  |
| FAD | up to 1.5fold stimulation | Bos taurus | |
| FMN | up to 1.5fold stimulation | Bos taurus | |
| additional information | enzyme activity increases at high ionic strength | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
3,5-diiodo-L-tyrosine | pH 7.4, 38°C | Bos taurus | |
| 0.05 | - |
3-iodo-L-tyrosine | pH 7.4, 38°C | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Bos taurus | - |
- |
| mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22300 | - |
2 * 22300, SDS-PAGE | Bos taurus |
| 42000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thyroid gland | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7800 | - |
pH 7.4, 38°C | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15 to -20°C in 10 mM phosphate buffer, stable for months | Bos taurus |
| 0 to 4°C in 10 mM phosphate buffer, stable for weeks | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,5-diiodo-L-tyrosine + NADPH + H+ | - |
Bos taurus | 3-iodo-L-tyrosine + NADP+ + I- | - |
? | |
| 3-iodo-L-tyrosine + NADPH + H+ | - |
Bos taurus | L-tyrosine + NADP+ + I- | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 22300, SDS-PAGE | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
3,5-diiodo-L-tyrosine | pH 7.4, 38°C | Bos taurus | |
| 18 | - |
3-iodo-L-tyrosine | pH 7.4, 38°C | Bos taurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | 1 mol of FMN per mol of protein | Bos taurus | |
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Release 2023.1 (January 2023)
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