BRENDA - Enzyme Database
show all sequences of 1.2.5.2

Aldehyde dehydrogenase from acetic acid bacteria, membrane-bound

Ameyama, M.; Adachi, O.; Methods Enzymol. 89, 491-497 (1982)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.9
-
acetaldehyde
-
Acetobacter aceti
3.3
-
acetaldehyde
-
Gluconobacter oxydans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
137000
-
3 components, MW 78000 dehydrogenase protein, MW 45000 cytochrome component, MW 14000 component with unknown function, SDS-PAGE
Acetobacter aceti
140000
-
2 components, MW 86000 dehydrogenase protein, MW 55000 cytochrome c, SDS-PAGE
Gluconobacter oxydans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetaldehyde + a quinone + H2O
Gluconobacter oxydans
-
acetate + a quinol
-
Gluconobacter oxydans
?
acetaldehyde + a quinone + H2O
Acetobacter aceti
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
acetate + a quinol
-
Acetobacter aceti
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetobacter aceti
-
-
-
Gluconobacter oxydans
-
-
-
Purification (Commentary)
Commentary
Organism
solubilization with Triton X-100, DEAE-cellulose, CM-cellulose
Gluconobacter oxydans
solubilization with Triton X-100/cetylpyridinium chloride, PEG precipitation, DEAE-cellulose, hydroxyapatite
Acetobacter aceti
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
430
-
-
Gluconobacter oxydans
654
-
-
Acetobacter aceti
Storage Stability
Storage Stability
Organism
4°C, 1% Triton X-100, 10 mM benzaldehyde, pH 4.0, several weeks, no loss of activity
Acetobacter aceti
4°C, 1% Triton X-100, 10 mM benzaldehyde, pH 4.0, several weeks, no loss of activity
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + a quinone + H2O
-
288471
Gluconobacter oxydans
acetate + a quinol
-
288471
Gluconobacter oxydans
?
acetaldehyde + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
acetate + a quinol
-
288471
Acetobacter aceti
?
benzaldehyde + a quinone + H2O
-
288471
Gluconobacter oxydans
benzoate + a quinol
-
288471
Gluconobacter oxydans
?
benzaldehyde + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors, 5% activity of acetaldehyde oxidation
288471
Acetobacter aceti
benzoate + a quinol
-
288471
Acetobacter aceti
?
butanal + a quinone + H2O
-
288471
Gluconobacter oxydans
butanoate + a quinone
-
288471
Gluconobacter oxydans
?
butanal + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
butanoate + a quinone
-
288471
Acetobacter aceti
?
hexanal + a quinone + H2O
-
288471
Gluconobacter oxydans
hexanoate + a quinol
-
288471
Gluconobacter oxydans
?
hexanal + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
hexanoate + a quinol
-
288471
Acetobacter aceti
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
-
Gluconobacter oxydans
5
-
-
Acetobacter aceti
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Acetobacter aceti
pyrroloquinoline quinone
-
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
-
Acetobacter aceti
pyrroloquinoline quinone
-
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.9
-
acetaldehyde
-
Acetobacter aceti
3.3
-
acetaldehyde
-
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
137000
-
3 components, MW 78000 dehydrogenase protein, MW 45000 cytochrome component, MW 14000 component with unknown function, SDS-PAGE
Acetobacter aceti
140000
-
2 components, MW 86000 dehydrogenase protein, MW 55000 cytochrome c, SDS-PAGE
Gluconobacter oxydans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetaldehyde + a quinone + H2O
Gluconobacter oxydans
-
acetate + a quinol
-
Gluconobacter oxydans
?
acetaldehyde + a quinone + H2O
Acetobacter aceti
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
acetate + a quinol
-
Acetobacter aceti
?
Purification (Commentary) (protein specific)
Commentary
Organism
solubilization with Triton X-100, DEAE-cellulose, CM-cellulose
Gluconobacter oxydans
solubilization with Triton X-100/cetylpyridinium chloride, PEG precipitation, DEAE-cellulose, hydroxyapatite
Acetobacter aceti
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
430
-
-
Gluconobacter oxydans
654
-
-
Acetobacter aceti
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 1% Triton X-100, 10 mM benzaldehyde, pH 4.0, several weeks, no loss of activity
Acetobacter aceti
4°C, 1% Triton X-100, 10 mM benzaldehyde, pH 4.0, several weeks, no loss of activity
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + a quinone + H2O
-
288471
Gluconobacter oxydans
acetate + a quinol
-
288471
Gluconobacter oxydans
?
acetaldehyde + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
acetate + a quinol
-
288471
Acetobacter aceti
?
benzaldehyde + a quinone + H2O
-
288471
Gluconobacter oxydans
benzoate + a quinol
-
288471
Gluconobacter oxydans
?
benzaldehyde + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors, 5% activity of acetaldehyde oxidation
288471
Acetobacter aceti
benzoate + a quinol
-
288471
Acetobacter aceti
?
butanal + a quinone + H2O
-
288471
Gluconobacter oxydans
butanoate + a quinone
-
288471
Gluconobacter oxydans
?
butanal + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
butanoate + a quinone
-
288471
Acetobacter aceti
?
hexanal + a quinone + H2O
-
288471
Gluconobacter oxydans
hexanoate + a quinol
-
288471
Gluconobacter oxydans
?
hexanal + a quinone + H2O
potassium ferricyanide, phenazine methosulfate, nitro blue tetrazolium or dichlorophenol-indophenol as artificial electron acceptors
288471
Acetobacter aceti
hexanoate + a quinol
-
288471
Acetobacter aceti
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
-
Gluconobacter oxydans
5
-
-
Acetobacter aceti
Other publictions for EC 1.2.5.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731507
Aquino Neto
High current density PQQ-depen ...
Gluconobacter oxydans, Gluconobacter oxydans DSM 3504
Biosens. Bioelectron.
72
247-254
2015
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
731204
Zeiser
Characterization of a periplas ...
Sphingomonas wittichii, Sphingomonas wittichii DSM 6014
Appl. Microbiol. Biotechnol.
98
2067-2079
2014
-
-
1
-
-
-
4
1
1
-
5
-
-
7
-
-
-
-
-
-
9
-
15
2
1
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
4
-
1
1
-
5
-
-
-
-
-
-
-
9
-
15
2
1
-
-
-
1
-
1
-
-
1
1
-
1
1
724837
Aquino Neto
-
Direct electron transfer-based ...
Gluconobacter sp., Gluconobacter sp. 33 (DSM Z 3504)
Electrochim. Acta
87
323-329
2013
-
-
-
-
-
-
-
1
-
-
3
-
-
2
-
-
1
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1
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2
6
1
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1
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1
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2
6
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
731035
Xu
-
Investigating the impact of mu ...
Gluconobacter oxydans, Gluconobacter oxydans DSM 3504
ACS Catal.
3
1756-1763
2013
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
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1
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731999
Gomez-Manzo
Molecular and catalytic proper ...
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus ATCC 49037
J. Bacteriol.
192
5718-5724
2010
-
-
-
-
-
-
2
1
1
-
3
-
-
2
-
-
1
-
-
-
-
4
14
1
-
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-
1
2
-
-
3
-
1
-
-
-
-
3
-
-
-
-
2
-
1
1
-
3
-
-
-
-
1
-
-
-
4
14
1
-
-
-
1
2
-
-
1
-
-
-
-
1
1
687166
Gomez-Manzo
The PQQ-alcohol dehydrogenase ...
Gluconacetobacter diazotrophicus
Int. J. Food Microbiol.
125
71-78
2008
-
-
-
-
-
-
-
-
1
-
3
1
-
1
-
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1
-
-
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1
-
5
1
-
-
1
-
1
1
-
2
-
1
-
-
-
-
2
-
-
-
-
-
-
-
1
-
3
1
-
-
-
1
-
-
1
-
5
1
-
-
1
-
1
1
-
1
-
-
-
-
-
-
685650
Shinagawa
A novel type of formaldehyde-o ...
Acetobacter sp., Acetobacter sp. SKU 14
Biosci. Biotechnol. Biochem.
70
850-857
2006
-
-
-
-
-
-
-
1
2
-
3
-
-
3
-
-
1
-
-
1
1
-
15
2
1
1
3
-
1
1
6
2
-
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-
2
-
-
-
-
-
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1
2
-
3
-
-
-
-
1
-
1
1
-
15
2
1
1
3
-
1
1
6
-
-
-
-
-
-
-
687366
Vangnai
Two distinct alcohol dehydroge ...
Thauera butanivorans
J. Bacteriol.
184
1916-1924
2002
1
-
1
-
1
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1
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1
1
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1
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3
1
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2
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1
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1
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3
1
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-
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389961
Zarnt
Catalytic and molecular proper ...
Cupriavidus necator, Cupriavidus necator Bo
J. Bacteriol.
183
1954-1960
2001
-
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1
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3
5
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1
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4
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10
1
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1
3
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1
1
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3
3
5
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1
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10
1
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688853
Vangnai
An inducible 1-butanol dehydro ...
Thauera butanivorans
Microbiology
147
745-756
2001
2
-
-
-
-
-
-
1
1
1
2
2
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1
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1
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1
4
4
1
10
1
1
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2
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1
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2
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1
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2
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1
1
4
4
1
10
1
1
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2
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1
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684539
Zarnt
Degradation of tetrahydrofurfu ...
Cupriavidus necator
Appl. Environ. Microbiol.
63
4891-4898
1997
1
-
-
-
-
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1
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1
2
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1
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6
1
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1
6
1
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1
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687362
Toyama
Three distinct quinoprotein al ...
Pseudomonas putida, Pseudomonas putida HK5
J. Bacteriol.
177
2442-2450
1995
3
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1
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1
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6
6
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9
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1
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3
3
-
28
2
-
-
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2
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3
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3
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3
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6
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1
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3
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6
6
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3
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3
4
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28
3
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3
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3
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-
-
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389935
Görisch
Quinoprotein ethanol dehydroge ...
Pseudomonas aeruginosa, Pseudomonas putida
Antonie van Leeuwenhoek
56
35-45
1989
-
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2
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2
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2
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2
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7
2
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2
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2
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7
2
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288470
Adachi
-
Preparation of cells and cytop ...
Acetobacter aceti
Agric. Biol. Chem.
52
2083-2084
1988
-
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1
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1
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1
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661296
Rupp
Purification, crystallisation ...
Pseudomonas aeruginosa
Biol. Chem. Hoppe-Seyler
369
431-439
1988
-
-
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1
-
-
-
1
-
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3
-
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1
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1
-
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4
1
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1
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1
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1
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3
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1
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4
1
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1
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389953
Groen
Quinohaemoprotein alcohol dehy ...
Comamonas testosteroni
Biochem. J.
234
611-615
1986
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1
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5
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288472
Hommel
-
A pyridine nucleotide-independ ...
Acetobacter pasteurianus
FEMS Microbiol. Lett.
22
139-142
1984
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1
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1
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5
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9
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1
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1
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3
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2
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2
2
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2
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2
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2
2
8
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2
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2
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2
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2
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2
2
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2
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2
2
8
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2
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1
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1
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1
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1
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1
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1
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1
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1
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