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Literature summary for 1.2.1.9 extracted from

  • Iddar, A.; Valverde, F.; Serrano, A.; Soukri, A.
    Expression, purification, and characterization of recombinant nonphosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Clostridium acetobutylicum (2002), Protein Expr. Purif., 25, 519-526.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL-21 Clostridium acetobutylicum

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 1 mM, 76% inhibition Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
NADP+ pH 8.5, 25┬░C Clostridium acetobutylicum
0.545
-
D-Glyceraldehyde-3-phosphate pH 8.5, 25┬░C Clostridium acetobutylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
4 * 50000, SDS-PAGE Clostridium acetobutylicum
204000
-
non-denaturing PAGE Clostridium acetobutylicum

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
ATCC 824
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
75
-
-
Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde-3-phosphate + NADP+
-
Clostridium acetobutylicum D-3-phosphoglycerate + NADPH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Clostridium acetobutylicum

Synonyms

Synonyms Comment Organism
nonphosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
-
Clostridium acetobutylicum

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
65
-
-
Clostridium acetobutylicum

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
70
-
10 min, inactivation above Clostridium acetobutylicum
95
-
10 min, complete inactivation Clostridium acetobutylicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Clostridium acetobutylicum

Cofactor

Cofactor Comment Organism Structure
NADP+ absolute specificity Clostridium acetobutylicum

pI Value

Organism Comment pI Value Maximum pI Value
Clostridium acetobutylicum chromatofocusing
-
4.2