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Literature summary for 1.2.1.9 extracted from
- Purification of recombinant non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Streptococcus pyogenes expressed in E. coli (2003), Mol. Cell. Biochem., 247, 195-203.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression by functional complementation of the Escherichia coli gap mutant W3CG | Streptococcus pyogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 1 mM, 85% inhibition | Streptococcus pyogenes |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.385 | - |
NADP+ | pH 8.5, 25°C | Streptococcus pyogenes | |
| 0.666 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C | Streptococcus pyogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
4 * 55000, SDS-PAGE | Streptococcus pyogenes |
| 224000 | - |
non-denaturing PAGE | Streptococcus pyogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pyogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Streptococcus pyogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 143.2 | - |
- |
Streptococcus pyogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde-3-phosphate + NADP+ | - |
Streptococcus pyogenes | D-3-phosphoglycerate + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 55000, SDS-PAGE | Streptococcus pyogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase | - |
Streptococcus pyogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Streptococcus pyogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
10 min, inactivation above | Streptococcus pyogenes |
| 75 | - |
10 min, complete inactivation | Streptococcus pyogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Streptococcus pyogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 12 | pH 7.5: about 35% of maximal activity, pH 12.0: about 60% of maximal activity | Streptococcus pyogenes |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptococcus pyogenes | chromatofocusing | - |
4.9 |
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Release 2023.1 (January 2023)
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