General Stability | Organism |
---|---|
salt-tolerant enzyme retains 50% of its initial activity at 1.2 M K+ | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetaldehyde | 100 mM, 83% inhibition | Escherichia coli | |
benzaldehyde | 1 mM, 51% inhibition | Escherichia coli | |
Betaine aldehyde | above 0.5 mM | Escherichia coli | |
Butyrylcholine | 100 mM, 73% inhibition | Escherichia coli | |
choline | 100 mM, 78% inhibition | Escherichia coli | |
ethanolamine | 100 mM, 35% inhibition | Escherichia coli | |
glyceraldehyde | 100 mM, 83% inhibition | Escherichia coli | |
Hg2+ | 0.0001-0.0005 mM HgCl2 | Escherichia coli | |
iodoacetamide | 1 mM | Escherichia coli | |
iodoacetate | 1 mM | Escherichia coli | |
isopentanal | 1 mM, 84% inhibition | Escherichia coli | |
additional information | no substrate inhibition with NAD+ | Escherichia coli | |
N,N-dimethylethanolamine | 100 mM, 57% inhibition | Escherichia coli | |
N,N-dimethylglycine | 1 mM, 24% inhibition | Escherichia coli | |
N-Methylethanolamine | 100 mM, 50% inhibition | Escherichia coli | |
N-methylglycine | 1 mM, 24% inhibition | Escherichia coli | |
NADP+ | substrate inhibition above 10 mM | Escherichia coli | |
PCMB | 0.0001-0.0005 mM | Escherichia coli | |
phenylacetaldehyde | 1 mM, 54% inhibition | Escherichia coli | |
tetramethylammonium hydroxide | 100 mM, 57% inhibition | Escherichia coli | |
trimethylacetaldehyde | 100 mM, 89% inhibition | Escherichia coli | |
ZnCl2 | 0.0001-0.0005 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.099 | - |
NAD+ | - |
Escherichia coli | |
0.16 | - |
Betaine aldehyde | - |
Escherichia coli | |
0.4 | - |
NADP+ | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activation up to 0.15 M, decreases activity at higher concentrations | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Escherichia coli |
232000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | Escherichia coli | the salt tolerance is an essential property for the enzyme participating in the cellular synthesis of an osmoprotectant | betaine + NADH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
over-producing strain carrying the structural gene for the enzyme on the plasmid pBR322 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C, frozen in presence of 10 mM dithiothreitol, stable for several months without appreciable loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | highly specific for betaine aldehyde | Escherichia coli | betaine + NADH | - |
ir | |
betaine aldehyde + NAD+ + H2O | the salt tolerance is an essential property for the enzyme participating in the cellular synthesis of an osmoprotectant | Escherichia coli | betaine + NADH | - |
ir | |
betaine aldehyde + NADP+ + H2O | NAD+ is preferred over NADP+ | Escherichia coli | betaine + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ is preferred over NADP+ | Escherichia coli | |
NADP+ | NAD+ is preferred over NADP+ | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
isopentanal | - |
Escherichia coli | |
0.53 | - |
phenylacetaldehyde | - |
Escherichia coli | |
0.7 | - |
benzaladehyde | - |
Escherichia coli | |
4.5 | - |
trimethylacetaldehyde | - |
Escherichia coli | |
5.7 | - |
acetaldehyde | - |
Escherichia coli | |
7.4 | - |
glyceraldehyde | - |
Escherichia coli | |
11 | - |
choline | - |
Escherichia coli | |
14 | - |
Butyrylcholine | - |
Escherichia coli | |
26 | - |
tetramethylammonium hydroxide | - |
Escherichia coli | |
27 | - |
N,N-dimethylethanolamine | - |
Escherichia coli | |
40 | - |
N-Methylethanolamine | - |
Escherichia coli | |
78 | - |
ethanolamine | - |
Escherichia coli | |
130 | - |
N,N-dimethylglycine | - |
Escherichia coli | |
130 | - |
N-methylglycine | - |
Escherichia coli |