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show all sequences of 1.2.1.68

Purification and characterization of the coniferyl aldehyde dehydrogenase from Pseudomonas sp. strain HR199 and molecular characterization of the gene

Achterholt, S.; Priefert, H.; Steinbuchel, A.; J. Bacteriol. 180, 4387-4391 (1998)

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
production of vanillin
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
0.012
coniferyl aldehyde
-
Pseudomonas sp.
0.334
-
NAD+
-
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49500
-
alpha2, 2 * 49500, SDS-PAGE
Pseudomonas sp.
86000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
coniferyl aldehyde + H2O + NAD+
Pseudomonas sp.
involved in the eugenol catabolism
ferulate + NADH
-
Pseudomonas sp.
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
induction of enzyme synthesis by growth in eugenol-containing media
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
36.54
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
coniferyl aldehyde + H2O + NAD+
involved in the eugenol catabolism
288325
Pseudomonas sp.
ferulate + NADH
-
288325
Pseudomonas sp.
?
coniferyl aldehyde + H2O + NADP+
4.3% of the rate compared to NAD+ as electron acceptor
288325
Pseudomonas sp.
ferulate + NADPH
-
288325
Pseudomonas sp.
?
Subunits
Subunits
Commentary
Organism
dimer
alpha2, 2 * 49500, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
26
-
-
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
26
-
rapid decrease of activity at higher temperatures
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
-
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8.8
-
very unstable
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
Application (protein specific)
Application
Commentary
Organism
nutrition
production of vanillin
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
0.012
coniferyl aldehyde
-
Pseudomonas sp.
0.334
-
NAD+
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49500
-
alpha2, 2 * 49500, SDS-PAGE
Pseudomonas sp.
86000
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
coniferyl aldehyde + H2O + NAD+
Pseudomonas sp.
involved in the eugenol catabolism
ferulate + NADH
-
Pseudomonas sp.
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
induction of enzyme synthesis by growth in eugenol-containing media
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
36.54
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
coniferyl aldehyde + H2O + NAD+
involved in the eugenol catabolism
288325
Pseudomonas sp.
ferulate + NADH
-
288325
Pseudomonas sp.
?
coniferyl aldehyde + H2O + NADP+
4.3% of the rate compared to NAD+ as electron acceptor
288325
Pseudomonas sp.
ferulate + NADPH
-
288325
Pseudomonas sp.
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
alpha2, 2 * 49500, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
26
-
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
26
-
rapid decrease of activity at higher temperatures
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
-
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8.8
-
very unstable
Pseudomonas sp.
Other publictions for EC 1.2.1.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742102
Adeboye
A coniferyl aldehyde dehydrog ...
Pseudomonas sp. HR199, Pseudomonas sp. HR199 DSM 7063
Biores. Technol.
212
11-19
2016
-
-
1
-
1
-
-
-
-
-
-
2
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654275
Overhage
Highly efficient biotransforma ...
Pseudomonas sp.
Appl. Environ. Microbiol.
69
6569-6576
2003
-
1
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654264
Overhage
Biotransformation of eugenol t ...
Pseudomonas sp.
Appl. Environ. Microbiol.
68
4315-4321
2002
-
1
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288325
Achterholt
Purification and characterizat ...
Pseudomonas sp.
J. Bacteriol.
180
4387-4391
1998
-
1
-
-
-
-
-
2
-
-
2
1
-
2
-
-
1
-
-
1
1
-
2
1
1
-
1
-
1
-
1
1
-
-
-
-
1
-
1
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
1
1
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-