Information on EC 1.2.1.68 - coniferyl-aldehyde dehydrogenase

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The expected taxonomic range for this enzyme is: Pseudomonas

EC NUMBER
COMMENTARY hide
1.2.1.68
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RECOMMENDED NAME
GeneOntology No.
coniferyl-aldehyde dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
coniferyl aldehyde + H2O + NAD(P)+ = ferulate + NAD(P)H + 2 H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ferulate and sinapate biosynthesis
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Phenylpropanoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
coniferyl aldehyde:NAD(P)+ oxidoreductase
Also oxidizes other aromatic aldehydes, but not aliphatic aldehydes.
CAS REGISTRY NUMBER
COMMENTARY hide
208540-41-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Pseudomonas sp. HR199 DSM 7063
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
coniferaldehyde + CoA + NADP+
feruloyl-CoA + NADPH + H+
show the reaction diagram
coniferyl aldehyde + H2O + NAD+
ferulate + NADH
show the reaction diagram
coniferyl aldehyde + H2O + NADP+
ferulate + NADPH
show the reaction diagram
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4.3% of the rate compared to NAD+ as electron acceptor
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
coniferaldehyde + CoA + NADP+
feruloyl-CoA + NADPH + H+
show the reaction diagram
coniferyl aldehyde + H2O + NAD+
ferulate + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007 - 0.012
coniferyl aldehyde
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0.334
NAD+
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
induction of enzyme synthesis by growth in eugenol-containing media
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49500
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alpha2, 2 * 49500, SDS-PAGE
86000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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alpha2, 2 * 49500, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.8
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very unstable
288325
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26
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rapid decrease of activity at higher temperatures
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, chromatography techniques
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant expression of the enzyme in Saccharomyces cerevisiae strain CEN.PK102-3A, deficient for acetaldehyde dehydrogenase Ald5 via deletion of ALD5, CALDH enzyme expression leads to increased coniferyl aldehyde conversion in the recombinant yeast strain. Effect of the conversion of coniferyl aldehyde on the lag phase of recombinant strains, overview
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
nutrition
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production of vanillin
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