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Literature summary for 1.2.1.67 extracted from
- Molecular cloning and characterization of vanillin dehydrogenase from Streptomyces sp. NL15-2K (2018), BMC Microbiol., 18, 154 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces sp. NL15-2K |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 1 mM | Streptomyces sp. NL15-2K |  |
| Cu2+ | 79% inhibition at 1 mM | Streptomyces sp. NL15-2K | |
| additional information | not affected by Co2+, Fe2+, Mg2+, Mn2+, and Fe3+ | Streptomyces sp. NL15-2K | |
| Zn2+ | 15% inhibition at 1 mM | Streptomyces sp. NL15-2K | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00847 | - |
Vanillin | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
| 0.566 | - |
Salicylaldehyde | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
| 0.647 | - |
NAD+ | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 214000 | - |
gel filtration | Streptomyces sp. NL15-2K |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. NL15-2K | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography, Resource Phe column chromatography, and Mono Q column chromatography | Streptomyces sp. NL15-2K |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxybenzaldehyde + NAD+ + H2O | 56% activity compared to vanillin | Streptomyces sp. NL15-2K | 4-hydroxybenzoate + NADH + 2 H+ | - |
? | |
| anisaldehyde + NAD+ + H2O | 15% activity compared to vanillin | Streptomyces sp. NL15-2K | anisoate + NADH + 2 H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | 79% activity compared to vanillin | Streptomyces sp. NL15-2K | benzoate + NADH + 2 H+ | - |
? | |
| isovanillin + NAD+ + H2O | 49% activity compared to vanillin | Streptomyces sp. NL15-2K | isovanillate + NADH + 2 H+ | - |
? | |
| additional information | no activity with NADP+ or syringaldehyde | Streptomyces sp. NL15-2K | ? | - |
- |
|
| protocatechualdehyde + NAD+ + H2O | 91% activity compared to vanillin | Streptomyces sp. NL15-2K | protocatechuate + NADH + 2 H+ | - |
? | |
| salicylaldehyde + NAD+ + H2O | 48% activity compared to vanillin | Streptomyces sp. NL15-2K | salicylate + NADH + 2 H+ | - |
? | |
| vanillin + NAD+ + H2O | 100% activity | Streptomyces sp. NL15-2K | vanillate + NADH + 2 H+ | - |
? | |
| veratraldehyde + NAD+ + H2O | 12% activity compared to vanillin | Streptomyces sp. NL15-2K | veratrate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 56000, SDS-PAGE | Streptomyces sp. NL15-2K |
| homotetramer | 4 * 51705, calculated from amino acid sequence | Streptomyces sp. NL15-2K |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| VDH | - |
Streptomyces sp. NL15-2K |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Streptomyces sp. NL15-2K |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | after 10 min at 20-70°C, the enzyme activity is retained up to 40°C but declines sharply above 45°C, and the enzyme solution incubated at 50°C becomes slightly clouded | Streptomyces sp. NL15-2K |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.46 | - |
Vanillin | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
| 14.91 | - |
Salicylaldehyde | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
| 23.45 | - |
NAD+ | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Streptomyces sp. NL15-2K |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | at all pH ranges tested, the enzyme loses more than 40% of its initial activity after storage at 4°C for 15 h, and the enzyme solution becomes clouded after storage at pH 5.0 or less | Streptomyces sp. NL15-2K |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Streptomyces sp. NL15-2K | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces sp. NL15-2K | calculated from amino acid sequence | - |
5 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
Salicylaldehyde | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
| 36 | - |
NAD+ | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
| 1110 | - |
Vanillin | at pH 9.5 and 45°C | Streptomyces sp. NL15-2K | |
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