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Information on EC 1.2.1.67 - vanillin dehydrogenase
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EC Tree
1.2.1.67 vanillin dehydrogenaseSpecify your search results
Word Map
- 1.2.1.67
-
vanillic
- virginia
-
vermont
- feruloyl-coa
- lentinan
-
25ohvd3
-
pseudonocardia
-
underserved
- autotrophica
-
lignin-derived
- synthesis
- nutrition
- biotechnology
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
vdh, vanillin dehydrogenase, aromatic aldehyde dehydrogenase, syringaldehyde dehydrogenase, slg_28320, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CD36-03230
Cd36-03230p
LigV
vanillin dehydrogenase
VDH
VDH1
YfmT
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
vanillin + NAD+ + H2O = vanillate + NADH + H+
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
vanillin:NAD+ oxidoreductase
-
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CAS REGISTRY NUMBER
COMMENTARY
189767-93-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-ethoxy-4-hydroxybenzaldehyde + NAD+ + H2O
3-ethoxy-4-hydroxybenzoate + NADH + 2 H+
-
-
-
-
?
3-hydroxy-4-methoxybenzaldehyde + NAD+ + H2O
3-hydroxy-4-methoxybenzoate + NADH + 2 H+
-
-
-
-
?
anisaldehyde + NAD+ + H2O
anisoate + NADH + 2 H+
-
15% activity compared to vanillin
-
-
?
coniferyl aldehyde + NAD+ + H2O
(2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + NADH + H+
coniferyl aldehyde + NAD+ + H2O
ferulic acid + NADH + 2 H+
-
-
-
?
isovanillin + NAD+ + H2O
isovanillate + NADH + 2 H+
-
49% activity compared to vanillin
-
-
?
m-anisaldehyde + NAD+ + H2O
m-anisate + NADH + 2 H+
-
-
-
?
syringaldehyde + NADP+ + H2O
syringate + NADPH + H+
high activity
-
-
?
vanillin + NAD+ + H2O
vanillate + NADH + H+
best substrate
-
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
3,4-dihydroxybenzoate + NADH + 2 H+
-
-
-
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
3,4-dihydroxybenzoate + NADH + 2 H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
3-hydroxybenzoate + NADH + 2 H+
-
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
3-hydroxybenzoate + NADH + 2 H+
-
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
3-hydroxybenzoate + NADH + 2 H+
-
-
-
?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
no reduction of vanillic acid to vanillin in crude cell extract observed, metabolism of ferulic acid to vanillic acid
-
ir
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
no reduction of vanillic acid to vanillin in crude cell extract observed, metabolism of ferulic acid to vanillic acid
-
ir
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
Streptomyces viridosporus T7A / ATCC 39115
-
-
-
r
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + 2 H+
-
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + 2 H+
-
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + 2 H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + 2 H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + 2 H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + 2 H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + 2 H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + 2 H+
-
56% activity compared to vanillin
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + 2 H+
-
70% relative activity compared to vanillin
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + 2 H+
-
70% relative activity compared to vanillin
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + 2 H+
-
77% relative activity compared to vanillin
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + 2 H+
-
77% relative activity compared to vanillin
-
-
?
5-hydroxymethylfurfural + NAD+ + H2O
5-hydroxymethyl-2-furancarboxylic acid + NADH + 2 H+
-
the tolerance of the recombinant enzyme toward 5-hydroxymethylfurfural is up to 200 mM
-
-
?
5-hydroxymethylfurfural + NAD+ + H2O
5-hydroxymethyl-2-furancarboxylic acid + NADH + 2 H+
Comamonas testosteroni SC1588
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the tolerance of the recombinant enzyme toward 5-hydroxymethylfurfural is up to 200 mM
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + 2 H+
66% activity in comparison to vanillin
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + 2 H+
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + 2 H+
-
79% activity compared to vanillin
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
-
-
-
?
cinnamaldehyde + NAD+ + H2O
cinnamate + NADH + 2 H+
-
-
-
?
cinnamaldehyde + NAD+ + H2O
cinnamate + NADH + 2 H+
-
-
-
?
coniferyl aldehyde + NAD+ + H2O
(2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + NADH + H+
activity toward coniferyl aldehyde is less than 5% of that toward vanillin
-
-
?
coniferyl aldehyde + NAD+ + H2O
(2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + NADH + H+
activity toward coniferyl aldehyde is less than 5% of that toward vanillin
-
-
?
cyclohexanecarboxyaldehyde + NAD+ + H2O
benzoate + NADH + 2 H+
-
-
-
?
cyclohexanecarboxyaldehyde + NAD+ + H2O
benzoate + NADH + 2 H+
-
-
-
?
isovanillin + NADP+ + H2O
isovanillate + NADPH + 2 H+
-
48% relative activity compared to vanillin
-
-
?
isovanillin + NADP+ + H2O
isovanillate + NADPH + 2 H+
-
48% relative activity compared to vanillin
-
-
?
isovanillin + NADP+ + H2O
isovanillate + NADPH + 2 H+
-
285% relative activity compared to vanillin
-
-
?
isovanillin + NADP+ + H2O
isovanillate + NADPH + 2 H+
-
285% relative activity compared to vanillin
-
-
?
m-anisaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH + H+
-
-
-
?
m-anisaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH + H+
-
-
-
?
p-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
-
-
-
?
p-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
-
-
-
?
protocatechualdehyde + NAD+ + H2O
protocatechuate + NADH + 2 H+
-
-
-
?
protocatechualdehyde + NAD+ + H2O
protocatechuate + NADH + 2 H+
-
91% activity compared to vanillin
-
-
?
protocatechualdehyde + NAD+ + H2O
protocatechuate + NADH + H+
-
-
-
?
protocatechualdehyde + NAD+ + H2O
protocatechuate + NADH + H+
-
-
-
?
protocatechualdehyde + NADP+ + H2O
protocatechuate + NADPH + 2 H+
-
94% relative activity compared to vanillin
-
-
?
protocatechualdehyde + NADP+ + H2O
protocatechuate + NADPH + 2 H+
-
94% relative activity compared to vanillin
-
-
?
protocatechualdehyde + NADP+ + H2O
protocatechuate + NADPH + 2 H+
-
146% relative activity compared to vanillin
-
-
?
protocatechualdehyde + NADP+ + H2O
protocatechuate + NADPH + 2 H+
-
146% relative activity compared to vanillin
-
-
?
salicylaldehyde + NAD+ + H2O
salicylate + NADH + 2 H+
-
-
-
?
salicylaldehyde + NAD+ + H2O
salicylate + NADH + 2 H+
-
48% activity compared to vanillin
-
-
?
syringaldehyde + NAD+ + H2O
syringate + NADH + H+
-
-
-
?
syringaldehyde + NAD+ + H2O
syringate + NADH + H+
very low activity
-
-
?
syringaldehyde + NAD+ + H2O
syringate + NADH + H+
high activity
-
-
?
syringaldehyde + NAD+ + H2O
syringate + NADH + H+
from syringyl lignin degradation of hardwood
-
-
?
syringaldehyde + NAD+ + H2O
syringate + NADH + H+
very low activity, from syringyl lignin degradation of hardwood
-
-
?
syringaldehyde + NAD+ + H2O
syringate + NADH + H+
activity toward syringaldehyde is less than 5% of that toward vanillin
-
-
?
syringaldehyde + NADP+ + H2O
syringate + NADPH + 2 H+
-
29% relative activity compared to vanillin
-
-
?
syringaldehyde + NADP+ + H2O
syringate + NADPH + 2 H+
-
29% relative activity compared to vanillin
-
-
?
syringaldehyde + NADP+ + H2O
syringate + NADPH + 2 H+
-
23% relative activity compared to vanillin
-
-
?
syringaldehyde + NADP+ + H2O
syringate + NADPH + 2 H+
-
23% relative activity compared to vanillin
-
-
?
vanillin + NAD+ + H2O
vanillate + NADH + 2 H+
no enzyme activity observed in the absence of NAD+, no oxidation of vanillin detectable when NADP+ used as cofactor
-
-
?
vanillin + NAD+ + H2O
vanillate + NADH + 2 H+
-
higher activity with NAD+ than with NADP+, relative activity with NADP1 is approximately 10%
-
-
?
vanillin + NAD+ + H2O
vanillate + NADH + 2 H+
-
higher activity with NAD+ than with NADP+, relative activity with NADP1 is approximately 10%
-
-
?
vanillin + NAD+ + H2O
vanillate + NADH + 2 H+
vdh mutant Rhodococcus jostii RHA045 is unable to grow on 1 mM vanillin as the sole organic substrate but grows on 1 mM vanillate with kinetics similar to that of RHA1, mutant rescue by vdh harbouring pTipvdh plasmid
-
-
?
vanillin + NAD+ + H2O
vanillate + NADH + 2 H+
best substrate
-
-
?
vanillin + NAD+ + H2O
vanillate + NADH + 2 H+
from guaiacol lignin degradation of softwood
-
-
?
vanillin + NADP+ + H2O
vanillate + NADPH + 2 H+
-
no activity with NAD+, strain TA1 exhibits oxidation activity specifically under alkaline conditions
-
-
?
vanillin + NADP+ + H2O
vanillate + NADPH + 2 H+
-
no activity with NAD+, strain TA1 exhibits oxidation activity specifically under alkaline conditions
-
-
?
veratraldehyde + NAD+ + H2O
veratrate + NADH + H+
-
-
-
?
veratraldehyde + NAD+ + H2O
veratrate + NADH + H+
activity toward veratraldehyde is less than 5% of that toward vanillin
-
-
?
veratraldehyde + NAD+ + H2O
veratrate + NADH + H+
-
12% activity compared to vanillin
-
-
?
additional information
?
-
vdh deletion mutant loses its ability to grow on vanillin and does not show vanillin dehydrogenase activity, 2.3fold higher vanillin concentration in vdh mutants observed when ferulic acid is provided for biotransformation in a cultivation experiment
-
-
?
additional information
?
-
-
vdh deletion mutant loses its ability to grow on vanillin and does not show vanillin dehydrogenase activity, 2.3fold higher vanillin concentration in vdh mutants observed when ferulic acid is provided for biotransformation in a cultivation experiment
-
-
?
additional information
?
-
-
4-hydroxy-3-methoxycinnamaldehyde and cinnamaldehyde are poor substrates
-
-
?
additional information
?
-
-
4-hydroxy-3-methoxycinnamaldehyde and cinnamaldehyde are poor substrates
-
-
?
additional information
?
-
the enzyme has no detectable activity with vanillin suggesting that the annotation is incorrect. Molecular modeling of CD36-03230p demonstrates that it has an isoleucine residue (Ile156) at key position in the active site, instead of e.g. Met, further strengthening this hypothesis
-
-
?
additional information
?
-
the enzyme has no detectable activity with vanillin suggesting that the annotation is incorrect. Molecular modeling of CD36-03230p demonstrates that it has an isoleucine residue (Ile156) at key position in the active site, instead of e.g. Met, further strengthening this hypothesis
-
-
?
additional information
?
-
product dentification by mass spectroscopic analysis
-
-
?
additional information
?
-
-
product dentification by mass spectroscopic analysis
-
-
?
additional information
?
-
product dentification by mass spectroscopic analysis
-
-
?
additional information
?
-
-
the enzyme is involved in catabolism of ferulic acid
-
-
?
additional information
?
-
-
the enzyme is involved in catabolism of ferulic acid
-
-
?
additional information
?
-
enzyme DesV shows a broad range of activity against benzaldehyde derivatives, substrate specificity, overview
-
-
?
additional information
?
-
enzyme DesV shows a broad range of activity against benzaldehyde derivatives, substrate specificity, overview
-
-
?
additional information
?
-
enzyme LigV shows a broad range of activity against benzaldehyde derivatives, substrate specificity, overview
-
-
?
additional information
?
-
enzyme LigV shows a broad range of activity against benzaldehyde derivatives, substrate specificity, overview
-
-
?
additional information
?
-
-
no activity with NADP+ or syringaldehyde
-
-
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
no reduction of vanillic acid to vanillin in crude cell extract observed, metabolism of ferulic acid to vanillic acid
-
ir
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
no reduction of vanillic acid to vanillin in crude cell extract observed, metabolism of ferulic acid to vanillic acid
-
ir
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
-
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
Streptomyces viridosporus T7A / ATCC 39115
-
-
-
r
syringaldehyde + NAD+ + H2O
syringate + NADH + H+
from syringyl lignin degradation of hardwood
-
-
?
syringaldehyde + NAD+ + H2O
syringate + NADH + H+
very low activity, from syringyl lignin degradation of hardwood
-
-
?
vanillin + NAD+ + H2O
vanillate + NADH + 2 H+
from guaiacol lignin degradation of softwood
-
-
?
additional information
?
-
-
the enzyme is involved in catabolism of ferulic acid
-
-
?
additional information
?
-
-
the enzyme is involved in catabolism of ferulic acid
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
DesV can oxidize SN in the presence of either NAD+ or NADP+;, but the specific activity is 7.3fold higher with NAD+ compared to NADP+
-
additional information
DesV can oxidize SN in the presence of either NAD+ or NADP+;, but the specific activity is 7.3fold higher with NAD+ compared to NADP+
-
additional information
LigV specifically requires NAD+, no activity with NADP+
-
additional information
LigV specifically requires NAD+, no activity with NADP+
-
additional information
structure-based multiple sequence alignment shows conserved residues at both NAD(P)+ binding and catalytic domains
-
additional information
the enzyme can utilize NAD+ and NADP+ as coenzymes with similar efficiency and shows no obvious difference toward NAD+ and NADP+
-
additional information
-
the enzyme can utilize NAD+ and NADP+ as coenzymes with similar efficiency and shows no obvious difference toward NAD+ and NADP+
-
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
no effect of EDTA on enzyme activity; no inhibition with 1 mM Cu2+, Fe3+, Fe2+, Zn2+, Mn2+, and Co2+
-
additional information
-
no inhibition with 1 mM Cu2+, Fe3+, Fe2+, Zn2+, Mn2+, and Co2+
-
additional information
-
not affected by Co2+, Fe2+, Mg2+, Mn2+, and Fe3+
-