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Literature summary for 1.2.1.59 extracted from

  • Aziz, I.; Rashid, N.; Ashraf, R.; Siddiqui, M.; Imanaka, T.; Akhtar, M.
    Pcal_0632, a phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Pyrobaculum calidifontis (2018), Extremophiles, 22, 121-129 .
    View publication on PubMed
Search for more literature for 1.2.1.59

Cloned(Commentary)

Cloned (Comment) Organism
gene gap, phylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21-CodonPlus (DE3)-RIL Pyrobaculum calidifontis

General Stability

General Stability Organism
the purified recombinant enzyme retains more than 60% of its activity even after an incubation of 72 h at room temperature in the presence of 6 M urea Pyrobaculum calidifontis

Inhibitors

Inhibitors Comment Organism Structure
GuHCl the enzyme retains more than 50% activity after 72 h of incubation in the presence of 4 M GuHCl, and 15% at 6 M GuHCl Pyrobaculum calidifontis
Urea the enzyme retains more than 50% activity after 72 h of incubation in the presence of 6 M urea, and 40% at 8 M urea Pyrobaculum calidifontis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Pyrobaculum calidifontis
0.125
-
 D-glyceraldehyde 3-phosphate recombinant enzyme, pH 7.0, 80°C, with NAD+ Pyrobaculum calidifontis
0.135
-
 NADP+ recombinant enzyme, pH 7.0, 80°C Pyrobaculum calidifontis
0.212
-
 NAD+ recombinant enzyme, pH 7.0, 80°C Pyrobaculum calidifontis
0.89
-
 D-glyceraldehyde 3-phosphate recombinant enzyme, pH 7.0, 80°C, with NADP+ Pyrobaculum calidifontis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pyrobaculum calidifontis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Pyrobaculum calidifontis
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Pyrobaculum calidifontis JCM 11548
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Pyrobaculum calidifontis VA1
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Pyrobaculum calidifontis
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Pyrobaculum calidifontis JCM 11548
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Pyrobaculum calidifontis VA1
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrobaculum calidifontis A3MTU1
-
-
Pyrobaculum calidifontis JCM 11548 A3MTU1
-
-
Pyrobaculum calidifontis VA1 A3MTU1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL Pyrobaculum calidifontis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pyrobaculum calidifontis 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pyrobaculum calidifontis JCM 11548 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pyrobaculum calidifontis VA1 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Pyrobaculum calidifontis 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Pyrobaculum calidifontis JCM 11548 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Pyrobaculum calidifontis VA1 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?
additional information highest activity in Bis-Tris buffer, lowest in imidazole-HCL, overview Pyrobaculum calidifontis ?
-
-
additional information highest activity in Bis-Tris buffer, lowest in imidazole-HCL, overview Pyrobaculum calidifontis JCM 11548 ?
-
-
additional information highest activity in Bis-Tris buffer, lowest in imidazole-HCL, overview Pyrobaculum calidifontis VA1 ?
-
-

Synonyms

Synonyms Comment Organism
GAP
-
 Pyrobaculum calidifontis
pcal_0632
-
 Pyrobaculum calidifontis
pcal_0632 locus name Pyrobaculum calidifontis
phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
 Pyrobaculum calidifontis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 recombinant enzyme Pyrobaculum calidifontis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 100 activity range, profile overview Pyrobaculum calidifontis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 the purified recombinant enzyme retains more than 60% of its activity even after an incubation of 72 h at room temperature in the presence of 6 M urea Pyrobaculum calidifontis
100
-
 half-life of the purified recombinant enzyme is 60 min Pyrobaculum calidifontis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 recombinant enzyme Pyrobaculum calidifontis

pH Range

pH Minimum pH Maximum Comment Organism
5 9 30% of maximal activity at pH 5.0, 40% at pH 9.0 Pyrobaculum calidifontis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pyrobaculum calidifontis
NADP+
-
 Pyrobaculum calidifontis