Cloned (Comment) | Organism |
---|---|
into the cloning vector pGEM-T Easy and subcloned into the expression vector pET14b | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
F471L | mutant | Mus musculus |
T1411C | mutant | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3',5-tri-iodothyronine | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
all-trans-retinal | wild-type | Mus musculus | |
0.0002 | - |
all-trans-retinal | F471L mutant | Mus musculus | |
0.0007 | - |
octanal | wild-type | Mus musculus | |
0.0009 | - |
octanal | F471L mutant | Mus musculus | |
0.0065 | - |
decanal | wild-type | Mus musculus | |
0.0221 | - |
hexanal | wild-type | Mus musculus | |
0.0431 | - |
NAD+ | F471L mutant | Mus musculus | |
0.0528 | - |
NAD+ | wild-type | Mus musculus | |
0.103 | - |
benzaldehyde | wild-type | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
retinal + NAD+ + H2O | Mus musculus | - |
retinoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9JHW9 | - |
- |
Purification (Comment) | Organism |
---|---|
using a nickel-chelating Sepharose and a S300 HR gel-filtration column | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | - |
Mus musculus | benzoate + NADH + H+ | - |
? | |
decanal + NAD+ + H2O | - |
Mus musculus | decanoate + NADH + H+ | - |
? | |
hexanal + NAD+ + H2O | - |
Mus musculus | hexanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | - |
Mus musculus | octanoate + NADH + H+ | - |
? | |
retinal + NAD+ + H2O | - |
Mus musculus | retinoate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RALDH3 | - |
Mus musculus |
Retinal dehydrogenase | - |
Mus musculus |
retinaldehyde dehydrogenase 3 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
benzaldehyde | wild-type | Mus musculus | |
0.3 | - |
hexanal | wild-type | Mus musculus | |
0.4 | - |
decanal | wild-type | Mus musculus | |
3.1 | - |
NAD+ | F471L mutant | Mus musculus | |
3.3 | - |
NAD+ | wild-type | Mus musculus | |
3.9 | - |
retinal | all-trans-retinal, wild-type | Mus musculus | |
4.8 | - |
octanal | wild-type | Mus musculus | |
5.4 | - |
octanal | F471L mutant | Mus musculus | |
13 | - |
retinal | all-trans-retinal, F471L mutant | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
activity assay | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0377 | - |
3,3',5-tri-iodothyronine | - |
Mus musculus |