Literature summary for 1.2.1.30 extracted from

  • He, A.; Li, T.; Daniels, L.; Fotheringham, I.; Rosazza, J.P.N.
    Nocardia sp. carboxylic acid reductase: cloning, expression, and characterization of a new aldehyde oxidoreductase family (2004), Appl. Environ. Microbiol., 70, 1874-1881.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Nocardia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
128300
-
x * 128300, deduced from gene sequence, x * 132400, SDS-PAGE, recombinant enzyme carrying His-tag Nocardia sp.
132400
-
x * 128300, deduced from gene sequence, x * 132400, SDS-PAGE, recombinant enzyme carrying His-tag Nocardia sp.

Organism

Organism UniProt Comment Textmining
Nocardia sp. Q6RKB1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme with his-tag Nocardia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
pH 7.5, 25°C, recombinant enzyme with His-tag Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoate + NADPH + ATP best substrate Nocardia sp. benzaldehyde + NADP+ + AMP + phosphate
-
?
coniferic acid + NADPH + ATP
-
Nocardia sp. coniferyl aldehyde + NADP+ + AMP + phosphate
-
?
vanillic acid + NADPH + H+ + ATP
-
Nocardia sp. vanillin + NADP+ + AMP + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 128300, deduced from gene sequence, x * 132400, SDS-PAGE, recombinant enzyme carrying His-tag Nocardia sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Nocardia sp.
additional information no cofactor: NADH Nocardia sp.

pI Value

Organism Comment pI Value Maximum pI Value
Nocardia sp. calculated from amino acid sequence
-
4.9