Cloned (Comment) | Organism |
---|---|
gene bkdA, the BKDH complex from Streptomyces avermitilis cannot readily be stably expressed in Escherichia coli. This is mostly due to low yields of active, properly folded enzyme in a complex expression system. Codon optimization, recombinant expression of the enzyme complex in Escherichia coli strain BW25113 by selectively regulating individual subunit gene expression | Streptomyces avermitilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | improvement of the production of short branched-chain acyl-CoAs for avermectin biosynthesis by functional expression of the branched chain alpha-keto acid dehydrogenase complex (BKDH) from Streptomyces avermitilis, systematic optimization by selectively regulating individual subunit expression in Escherichia coli, codon optimization, method, overview. Synergic synthesis pathway of avermectin precursors. Codon optimization could improve the expression of BkdH and LpdA1 from pRB1a, we further coexpressed the bkdF and bkdG with wild-type or codon optimized bkdH and lpdA1 by constructing vectors pRB01 (wild-type) and pRB02 (optimized). The bkdF-bkdG and bkdH-lpdA1 gene clusters were placed under the control of two independent araBAD promoters in pRB1a. Genes bkdF and bkdG are respectively inserted close to the araBAD promoters so as to enhance their expression | Streptomyces avermitilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate + CoA + NAD+ | Streptomyces avermitilis | - |
2-methylpropanoyl-CoA + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | Q53592 | BKDH complex component E1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate + CoA + NAD+ | - |
Streptomyces avermitilis | 2-methylpropanoyl-CoA + CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bkdA | - |
Streptomyces avermitilis |
BKDH | - |
Streptomyces avermitilis |
branched chain alpha-keto acid dehydrogenase complex | - |
Streptomyces avermitilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Streptomyces avermitilis | |
NAD+ | - |
Streptomyces avermitilis | |
thiamine diphosphate | - |
Streptomyces avermitilis |
General Information | Comment | Organism |
---|---|---|
physiological function | the BKDH complex is a multienzyme complex composed of three catalytic components: a thiamine diphosphate-dependent decarboxylase/dehydrogenase (E1), a lipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3) | Streptomyces avermitilis |