Literature summary for 1.2.1.16 extracted from

Zheng, H.; Beliavsky, A.; Tchigvintsev, A.; Brunzelle, J.; Brown, G.; Flick, R.; Evdokimova, E.; Wawrzak, Z.; Mahadevan, R.; Anderson, W.; Savchenko, A.; Yakunin, A.
Structure and activity of the NAD(P)+-dependent succinate semialdehyde dehydrogenase YneI from Salmonella typhimurium (2013), Proteins Struct. Funct. Bioinform., 81, 1031-1041.

Search for more literature for 1.2.1.16

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of apoenzyme and in complex with NAD+, to 1.85 and 1.9 A resolution. Two domain protein with the active site located in the interdomain interface. The NAD+ molecule is bound in the long channel with its nicotinamide ring positioned close to the side chain of the catalytic Cys268	Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants	Comment	Organism
D426A	strong decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium
E442A	strong decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium
F138A	decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium
K160A	Lys160 contributes to the enzyme preference to NAD+, strong decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium
K160E	Lys160 contributes to the enzyme preference to NAD+, strong decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium
K92A	strong decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium
N193A	strong decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium
R145A	strong decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium
R428A	activity similar to wild-type	Salmonella enterica subsp. enterica serovar Typhimurium
S425A	decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium
W141A	strong decrease in activity	Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors	Comment	Organism	Structure
Succinic semialdehyde	substrate inhibition above 0.1 mM	Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.022	-	succinate semialdehyde	mutant R428A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.027	-	succinate semialdehyde	wild-type, cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.043	-	NAD+	wild-type, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.047	-	succinate semialdehyde	wild-type, cofactor NADP+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.12	-	succinate semialdehyde	mutant F138A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.14	-	succinate semialdehyde	mutant E442A, cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.15	-	NAD+	mutant N193A, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.22	-	succinate semialdehyde	mutant S425A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.23	-	succinate semialdehyde	mutant D426A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.48	-	NAD+	wild-type, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.77	-	succinate semialdehyde	mutant W141A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.4	-	NADP+	mutant N193A, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.49	-	succinate semialdehyde	mutant R145A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.6	-	succinate semialdehyde	mutant K92A, cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
10	-	NAD+	mutant K160A , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
11	-	NAD+	mutant K160E , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
26	-	NADP+	mutant K160A , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
27	-	NADP+	mutant K160E , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	Q8ZPI3	-	-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720	Q8ZPI3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinate semialdehyde + NAD+ + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium	succinate + NADH + 2 H+	-	?
succinate semialdehyde + NAD+ + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720	succinate + NADH + 2 H+	-	?
succinate semialdehyde + NADP+ + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium	succinate + NADPH + 2 H+	-	?
succinate semialdehyde + NADP+ + H2O	-	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720	succinate + NADPH + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
YneI	-	Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.3	-	succinate semialdehyde	mutant D426A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2	8	succinate semialdehyde	mutant R428A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.1	-	NADP+	mutant K160E , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.6	-	NADP+	mutant K160A , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
14	-	NAD+	mutant N193A, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
21	-	NADP+	mutant N193A, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
26	-	succinate semialdehyde	mutant F138A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
38	-	NAD+	wild-type, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
39	-	NAD+	mutant K160A , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
45	-	succinate semialdehyde	mutant R145A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
46	-	NAD+	wild-type, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
55	-	succinate semialdehyde	wild-type, cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
66	-	succinate semialdehyde	mutant W141A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
68	-	succinate semialdehyde	mutant S425A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
73	-	succinate semialdehyde	mutant K92A, cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
80	-	NAD+	mutant K160E , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
83	-	succinate semialdehyde	wild-type, cofactor NADP+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
164	-	succinate semialdehyde	mutant E442A, cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	affinity to NAD+ is 10 times higher than to NADP+	Salmonella enterica subsp. enterica serovar Typhimurium
NADP+	affinity to NAD+ is 10 times higher than to NADP+	Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.1	-	NADP+	mutant K160A , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
0.77	-	NADP+	mutant K160E , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
1.3	-	succinate semialdehyde	mutant D426A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
3.8	-	NAD+	mutant K160A , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
7.3	-	NAD+	mutant K160E , pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
8.6	-	NADP+	mutant N193A, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
18	-	succinate semialdehyde	mutant R145A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
28	-	succinate semialdehyde	mutant K92A, cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
86	-	succinate semialdehyde	mutant W141A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
95	-	NAD+	wild-type, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
97	-	NAD+	mutant N193A, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
210	-	succinate semialdehyde	mutant F138A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
310	-	succinate semialdehyde	mutant S425A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
880	-	NAD+	wild-type, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
1100	-	succinate semialdehyde	mutant E442A, cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
1300	-	succinate semialdehyde	mutant R428A , cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
1800	-	succinate semialdehyde	wild-type, cofactor NADP+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2000	-	succinate semialdehyde	wild-type, cofactor NAD+, pH 8.0, 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)