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Literature summary for 1.2.1.16 extracted from
- Archaeal aldehyde dehydrogenase ST0064 from Sulfolobus tokodaii, a paralog of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, is a succinate semialdehyde dehydrogenase (2013), Biosci. Biotechnol. Biochem., 77, 1344-1348.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sulfurisphaera tokodaii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S434A | kinetic data show no significant difference from wild-type enzyme | Sulfurisphaera tokodaii |
| S434D | kinetic data show no significant difference from wild-type enzyme | Sulfurisphaera tokodaii |
| S434E | kinetic data show no significant difference from wild-type enzyme | Sulfurisphaera tokodaii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme A | 1 mM, 15% inhibition | Sulfurisphaera tokodaii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
succinate semialdehyde | pH 8.0, 60°C, cosubstrate: NAD+ | Sulfurisphaera tokodaii | |
| 0.0029 | - |
succinate semialdehyde | pH 8.0, 60°C, cosubstrate: NADP+ | Sulfurisphaera tokodaii | |
| 0.043 | - |
NADP+ | pH 8.0, 60°C | Sulfurisphaera tokodaii | |
| 3.3 | - |
NAD+ | pH 8.0, 60°C | Sulfurisphaera tokodaii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
4 * 52000, SDS-PAGE | Sulfurisphaera tokodaii |
| 180000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | Q976X5 | - |
- |
| Sulfurisphaera tokodaii 7 | Q976X5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | neither propionaldehyde nor phthalaldehyde seves as substrate | Sulfurisphaera tokodaii | ? | - |
? | |
| additional information | neither propionaldehyde nor phthalaldehyde seves as substrate | Sulfurisphaera tokodaii 7 | ? | - |
? | |
| succinate semialdehyde + NAD+ + H2O | - |
Sulfurisphaera tokodaii | succinate + NADH + 2 H+ | - |
? | |
| succinate semialdehyde + NAD+ + H2O | - |
Sulfurisphaera tokodaii 7 | succinate + NADH + 2 H+ | - |
? | |
| succinate semialdehyde + NADP+ + H2O | - |
Sulfurisphaera tokodaii | succinate + NADPH + 2 H+ | - |
? | |
| succinate semialdehyde + NADP+ + H2O | - |
Sulfurisphaera tokodaii 7 | succinate + NADPH + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 52000, SDS-PAGE | Sulfurisphaera tokodaii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ST0064 | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Sulfurisphaera tokodaii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27.6 | - |
NADP+ | pH 8.0, 60°C | Sulfurisphaera tokodaii | |
| 29.4 | - |
succinate semialdehyde | pH 8.0, 40°C, cosubstrate: NAD+ | Sulfurisphaera tokodaii | |
| 30 | - |
succinate semialdehyde | pH 8.0, 40°C, cosubstrate: NADP+ | Sulfurisphaera tokodaii | |
| 51.2 | - |
NAD+ | pH 8.0, 60°C | Sulfurisphaera tokodaii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Sulfurisphaera tokodaii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | kcat/Km-value is comparable for succinate semialdehyde with NADP+ and for succinate semialdehyde with NAD+ | Sulfurisphaera tokodaii | |
| NADP+ | kcat/Km-value is comparable for succinate semialdehyde with NADP+ and for succinate semialdehyde with NAD+ | Sulfurisphaera tokodaii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15.5 | - |
NAD+ | pH 8.0, 60°C | Sulfurisphaera tokodaii | |
| 643 | - |
NADP+ | pH 8.0, 60°C | Sulfurisphaera tokodaii | |
| 10300 | - |
succinate semialdehyde | pH 8.0, 60°C, cosubstrate: NADP+ | Sulfurisphaera tokodaii | |
| 14700 | - |
succinate semialdehyde | pH 8.0, 60°C, cosubstrate: NAD+ | Sulfurisphaera tokodaii | |
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