Literature summary for 1.2.1.16 extracted from

Sanchez, M.; Alvarez, M.A.; Balana, R.; Garrido-Pertierra, A.
Properties and functions of two succinic-semialdehyde dehydrogenases from Pseudomonas putida (1988), Biochim. Biophys. Acta, 953, 249-257.

Search for more literature for 1.2.1.16

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	-	Pseudomonas putida
dithiothreitol	-	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: succinate, pyruvate	Pseudomonas putida
NADPH	-	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0073	-	succinate semialdehyde	pH 8.0, 30°C	Pseudomonas putida
0.107	-	NAD+	pH 8.0, 30°C	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53000	-	2 * 53000, SDS-PAGE	Pseudomonas putida
100000	-	gel filtration	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
succinate semialdehyde + NAD(P)+ + H2O = succinate + NAD(P)H + H+	mechanism is sequential, consistent with rapid-equilibrium ordered bi-bi system and a steady-state ordered bi-bi system	Pseudomonas putida	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
40.7	-	pH 8.0, 30°C	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinate semialdehyde + NAD+ + H2O	-	Pseudomonas putida	succinate + NADH + H+	-	?
succinate semialdehyde + NADP+ + H2O	at 9% of the rate with NAD+	Pseudomonas putida	succinate + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 53000, SDS-PAGE	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pseudomonas putida
NADP+	at 9% of the rate with NAD+	Pseudomonas putida

Expression

Organism	Comment	Expression
Pseudomonas putida	induction by growth on 4-hydroxyphenylacetate	additional information

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Release 2023.1 (January 2023)