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Literature summary for 1.2.1.16 extracted from

  • Saehuan, C.; Rojanarata, T.; Wiyakrutta, S.; McLeish, M.J.; Meevootisom, V.
    Isolation and characterization of a benzoylformate decarboxylase and a NAD+/NADP+-dependent benzaldehyde dehydrogenase involved in D-phenylglycine metabolism in Pseudomonas stutzeri ST-201 (2007), Biochim. Biophys. Acta, 1770, 1585-1592.
    View publication on PubMed
Search for more literature for 1.2.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0069
-
 benzaldehyde in the presence of NADP+, at 25°C and pH 8.5 Pseudomonas stutzeri
0.0071
-
 benzaldehyde in the presence of NAD+, at 25°C and pH 8.5 Pseudomonas stutzeri
0.997
-
 NAD+ in the presence of benzaldehyde, at 25°C and pH 8.5 Pseudomonas stutzeri
6.14
-
 NADP+ in the presence of benzaldehyde, at 25°C and pH 8.5 Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzaldehyde + NAD+ + H2O Pseudomonas stutzeri
-
 benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O Pseudomonas stutzeri ST-201
-
 benzoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
-
-
Pseudomonas stutzeri ST-201
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + NAD+ + H2O
-
 Pseudomonas stutzeri benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Pseudomonas stutzeri ST-201 benzoate + NADH + H+
-
 ?
benzaldehyde + NADP+ + H2O weaker reaction compared to NAD+ Pseudomonas stutzeri benzoate + NADPH + H+
-
 ?
benzaldehyde + NADP+ + H2O weaker reaction compared to NAD+ Pseudomonas stutzeri ST-201 benzoate + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
NAD+/NADP+-dependent benzaldehyde dehydrogenase
-
 Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4
-
 benzaldehyde in the presence of NADP+, at 25°C and pH 8.5 Pseudomonas stutzeri
40
-
 NADP+ in the presence of benzaldehyde, at 25°C and pH 8.5 Pseudomonas stutzeri
57
-
 benzaldehyde in the presence of NAD+, at 25°C and pH 8.5 Pseudomonas stutzeri
176
-
 NAD+ in the presence of benzaldehyde, at 25°C and pH 8.5 Pseudomonas stutzeri

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred cofactor Pseudomonas stutzeri
NADP+ NAD+ is preferred over NADP+ Pseudomonas stutzeri