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Literature summary for 1.2.1.16 extracted from
- Succinate semialdehyde dehydrogenase of Rhodopseudomonas spheroides which is active with both NADP+ and NAD+ (1971), J. Biochem., 70, 243-247.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl reagents | - |
Cereibacter sphaeroides |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
NADP+ | - |
Cereibacter sphaeroides | |
| 0.16 | 0.18 | succinate semialdehyde | either with NAD+ or with NADP+ | Cereibacter sphaeroides | |
| 0.8 | - |
NAD+ | - |
Cereibacter sphaeroides | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| column chromatography on DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex | Cereibacter sphaeroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate semialdehyde + NAD+ + H2O | - |
Cereibacter sphaeroides | succinate + NADH + H+ | - |
r | |
| succinate semialdehyde + NADP+ + H2O | - |
Cereibacter sphaeroides | succinate + NADPH + H+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.7 | - |
NAD+ | Cereibacter sphaeroides |
| 9.9 | - |
NADP+ | Cereibacter sphaeroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | 10.6 | with NAD+, at pH 8.7: 45% of activity maximum, at pH 10.6: about 50% of activity maximum | Cereibacter sphaeroides |
| 8.7 | 10.9 | with NADP+, at pH 8.7: about 30% of activity maximum, at pH 10.9: about 65% of activity maximum | Cereibacter sphaeroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | enzyme is active both with NADP+ and NAD+, Vmax with NAD+ is considerably larger than that obtained with NADP+ | Cereibacter sphaeroides | |
| NADP+ | enzyme is active both with NADP+ and NAD+, Vmax with NAD+ is considerably larger than that obtained with NADP+ | Cereibacter sphaeroides | |
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Release 2023.1 (January 2023)
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