Any feedback?
Literature summary for 1.2.1.16 extracted from
- Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z (1976), Biochim. Biophys. Acta, 429, 55-62.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl reagents | beta-mercaptoethanol and dithiotreitol activate | Euglena gracilis |  |
General Stability
| General Stability | Organism |
|---|---|
| ethyleneglycol, 30%, stabilizes | Euglena gracilis |
| glycerol, 30%, stabilizes | Euglena gracilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Euglena gracilis | |
| glyoxylate | - |
Euglena gracilis | |
| N-ethylmaleimide | - |
Euglena gracilis | |
| p-chloromercuribenzoate | - |
Euglena gracilis | |
| potassium phosphate buffer | - |
Euglena gracilis | |
| Zn2+ | - |
Euglena gracilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
NADP+ | - |
Euglena gracilis | |
| 1.06 | - |
succinate semialdehyde | - |
Euglena gracilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | slight activation | Euglena gracilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Euglena gracilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation and DEAE-cellulose | Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.94 | - |
- |
Euglena gracilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, complete inactivation after 2 days | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malonic semialdehyde + NADP+ + H2O | hydrolyzed at 8.7% the rate of succinate semialdehyde | Euglena gracilis | malonate + NADPH + H+ | - |
? | |
| succinate semialdehyde + NADP+ + H2O | - |
Euglena gracilis | succinate + NADPH + H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | - |
Euglena gracilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
up to, in presence of 30% glycerol | Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Euglena gracilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | - |
Euglena gracilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | specific for | Euglena gracilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
