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Literature summary for 1.2.1.16 extracted from
- Enzymatic utilization of gamma-hydroxybutyric acid (1960), J. Biol. Chem., 235, 954-960.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| potassium arsenite | 75% inhibition, concentration 2 mM | Pseudomonas sp. |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
constitutive | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose chromatography | Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 3°C or -15°C, overnight, 100% loss of activity, without mercaptoethanol | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate semialdehyde + NADP+ + H2O | - |
Pseudomonas sp. | succinate + NADPH + H+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.5 | - |
Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas sp. | |
| NADP+ | reduces NAD+ at one-twelfth the rate of NADP+ | Pseudomonas sp. | |
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Release 2023.1 (January 2023)
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