Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a validated target for antimicrobial drug design | Aspergillus fumigatus |
Cloned (Comment) | Organism |
---|---|
gene AFUA_3G06830, codon-optimized, recombinant expression of C-terminally His-tagged enzyme ASADH in Escherichia coli strain BL21(DE3) | Aspergillus fumigatus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant C-terminally His-tagged enzyme, hanging drop vapour diffusion method, crystallization screening, mixing of 0.001 ml protein solution plus 0.001 ml reservoir solution containing 1.9 M ammonium sulfate, 100 mM Bis-Tris, pH 6.5, at 20°C, crystals are either soaked or cocrystallized with 5 mM NADP+ and with several moderate inhibitors that are identified from fragment library screening, crystal cryoprotection with reservoir solution containing 20% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 2.8-3.2 A resolution, modeling by molecular replacement using CaASADH, PDB ID 3hsk, as the search model | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + phosphate + NADP+ | Aspergillus fumigatus | - |
L-4-aspartyl phosphate + NADPH + H+ | - |
r | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | Aspergillus fumigatus ATCC MYA-4609 | - |
L-4-aspartyl phosphate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4WWR8 | - |
- |
Aspergillus fumigatus ATCC MYA-4609 | Q4WWR8 | - |
- |
no activity in Homo sapiens | - |
and other mammals | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme ASADH from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and dialysis | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Aspergillus fumigatus | L-4-aspartyl phosphate + NADPH + H+ | - |
r | |
L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Aspergillus fumigatus ATCC MYA-4609 | L-4-aspartyl phosphate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | enzyme AfASADH displays a crystallographic dimer | Aspergillus fumigatus |
More | enzyme secondary structure, crystal structure analysis, detailed overview | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
AFUA_3G06830 | - |
Aspergillus fumigatus |
ASADH | - |
Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Aspergillus fumigatus | |
NADPH | - |
Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
metabolism | aspartate-semialdehyde dehydrogenase catalyzes the reductive dephosphorylation of the substrate beta-aspartyl phosphate into aspartate semialdehyde, a key intermediate in the aspartate biosynthetic pathway and functions at a critical junction in the aspartate biosynthetic pathway | Aspergillus fumigatus |
additional information | active site structure analysis and comparison, detailed overview | Aspergillus fumigatus |
physiological function | the enzyme catalyzes the NADPH-dependent reductive dephosphorylation of 4-aspartyl phosphate to produce the key intermediate aspartate semialdehyde | Aspergillus fumigatus |